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  • 2000-2004  (14)
  • 1985-1989
  • 2001  (14)
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  • 2000-2004  (14)
  • 1985-1989
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  • 11
    Electronic Resource
    Electronic Resource
    Increased nitric oxide production in nasal epithelial cells from allergic patients – RT-PCR analysis and direct imaging by a fluorescence indicator: DAF-2 DA* (2001)
    Oxford, UK : Blackwell Science, Ltd
    Clinical & experimental allergy 31 (2001), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Nitric oxide (NO) is believed to participate in the regulation of airway clearance and non-specific cellular immunity. Recent studies have suggested that airway epithelial cells of allergic and non-allergic individuals may differ in their ability to produce this molecule.Objective The aim of this study was to detect the difference in NO production in human nasal epithelial cells between normal subjects and patients with perennial allergic rhinitis (AR), and to assess the relationship between the expression of nitric oxide synthase (NOS) isoforms and the severity of the disease.Methods Nasal epithelial cells were obtained from the inferior turbinate. The expression of mRNAs encoding constitutive endothelial NOS (eNOS) and inducible NOS (iNOS) was studied by reverse transcription-polymerase chain reaction (RT-PCR). Direct NO production in living cells was visualized and quantified by a fluorescent indicator, DAF-2 DA.Results RT-PCR analysis demonstrated that AR patients with a RAST score of 5 or 6 showed significant increases in the levels of iNOS mRNA and slight reductions in those of eNOS mRNA. Patients with a RAST score of 2–4 also revealed the same tendency however, the difference was not significant. DAF-2 DA imaging demonstrated that epithelial cells, especially the ciliated cells, produced a larger amount of NO than non-epithelial inflammatory cells. Preincubation with L-NAME resulted in an approximate 40% decrease in both groups.Conclusion These results directly indicate that nasal epithelial cells of AR patients overall produce higher levels of NO through the concomitant expression of different NOS isoforms. Continuous NO production by the epithelial cells in normal subjects further support the hypothesis that NO derived from epithelium may play dual roles in the regulation of nasal airway clearance and in the host defense. In addition, the use of DAF-2 DA provides a reliable method to visualize and quantify the direct NO production of living cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2001.01093.x
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    Paper (German National Licenses)
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  • 12
    Electronic Resource
    Electronic Resource
    A statistical model for cleavage fracture in notched specimens of C–Mn steel (2001)
    Oxford, UK : Blackwell Science Ltd
    Fatigue & fracture of engineering materials & structures 24 (2001), S. 0 
    Details
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A statistical model for cleavage fracture in notched specimens of C-Mn steel has been proposed. This model is based on a recently suggested physical model. This statistical model satisfactorily describes the distributions of the cumulative failure probability and failure probability density of 36 notched specimens fractured at various loads at test temperature of −196 °C. The minimum notch toughness has also been discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-2695.2001.00382.x
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    Paper (German National Licenses)
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  • 13
    Electronic Resource
    Electronic Resource
    Application of decohesive model with mixed damage scale in fracture analysis of composite materials (2001)
    Oxford, UK : Blackwell Science Ltd
    Fatigue & fracture of engineering materials & structures 24 (2001), S. 0 
    Details
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A decohesive model using a mixed damage scale and using the total fracture energy to simulate the fracture process of composite materials has been developed in this article. The model assumes a bilinear interfacial decohesion function and is incorporated into an interface finite element developed as a user subroutine in the commercial FEA package ABAQUS. In comparison with traditional numerical methods in fracture mechanics, this approach can automatically predict the failure load, crack path and the residual stiffness of bodies undergoing the fracture process. Applications given in this paper are simulation of a typical fracture test with a double cantilever beam (DCB) specimen; modelling a stiffened composite laminated panel under four-point bending and modelling a repaired composite sandwich panel under four-point bending. Good correlation was seen between modelling predictions and experimental results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-2695.2001.00441.x
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    Paper (German National Licenses)
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  • 14
    Electronic Resource
    Electronic Resource
    Candida albicans colonization on thermal cycled maxillofacial polymeric materials in vitro (2001)
    Oxford UK : Blackwell Science Ltd
    Journal of oral rehabilitation 28 (2001), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In the present study, the colonization of a single isolate of Canadida albicans on saliva-, serum-coated or protein free (uncoated), thermocycled (4–70 °C for 1 min, respectively; 0, 1000 and 10 000 times) 15 commercial maxillofacial materials were investigated, by adenosine triphosphate (ATP) analysis. In the case of control specimens (not thermocycled and uncoated), the fungal colonization appeared to depend upon the type of commercial products used. Thus, the lowest colonization was observed with additional silicone materials, soft acrylic liners with visible light curing, except for one product, whereas visible light curing liners comprising of single paste or single gel exhibited the highest colonization capacity, and cold cured acrylic liners exhibited the intermediate. However, the fungal colonization on the materials was significantly promoted both by thermal cycling (ANOVA; P 〈 0·01) and a layer of protein coating (saliva, P 〈 0·01; serum, P 〈 0·01). When the inter-relation between the fungal colonization and the surface hydrophobicity of the materials were analysed, fungal colonization on 1000- and 10 000-thermocycled materials correlated well with the contact angles of the materials (Student’s t-test, P 〈 0·01), being consistent with the thermodynamic theory. These results, taken together, suggest that the ageing of the materials and the biological fluids of the host promote yeast colonization on maxillofacial materials.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2842.2001.00685.x
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    Paper (German National Licenses)
    Fulltext
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