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  • 2000-2004  (10)
  • 1975-1979
  • 1970-1974
  • 2004  (10)
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  • 2000-2004  (10)
  • 1975-1979
  • 1970-1974
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  • 1
    Electronic Resource
    Electronic Resource
    An Analytical Method for Soy Saponins by HPLC/ELSD (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 69 (2004), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : A high-performance liquid chromatography/evaporative light scattering detection (HPLC/ELSD) method was developed to determine natural forms of soyasaponin B with (αg, βg, βa, γg, γya) and without (V, I, II, III, IV) 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP) in soybean. The coefficient of variance of intra-day assays was 〈 9.51% and that of inter-day assays was 〈 10.91%. The soyasaponin I was used as external standard for quantification of all soyasaponins because it was established that their ELSD signal responses were essentially the same under the specific operating conditions. The extraction conditions were optimized and the extract storage conditions were established for analysis of soyasaponin Bs in their natural state. The energy of activation of soyasaponin Pg was 40.76 kJ/mol. The recovery rates for soyasaponin I and II were 98.3% and 93.1%, respectively. The average of total soyasaponins content from 5 soybean samples was 3.37 μmol/g with ranges from 2.78 to 4.03 μmol/g. This method is simple and easy to use, and the sample preparation takes less time than existing methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2004.tb10988.x
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  • 2
    Electronic Resource
    Electronic Resource
    Decreased CD4+CD25+ T Cells in Peripheral Blood of Patients with Systemic Lupus Erythematosus (2004)
    Oxford, UK; Malden , USA : Blackwell Science Ltd
    Scandinavian journal of immunology 59 (2004), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Recent animal studies have shown that CD4+CD25+ T cells play a crucial role in the suppression of the immune response and that depletion of this subset of T cells might lead to development of autoimmune diseases. The aim of the present study was to investigate the levels of CD4+CD25+ T cells in the peripheral blood of patients with systemic lupus erythematosus (SLE). Ninety-four SLE patients, 52 patients with rheumatoid arthritis (RA) and 50 age- and gender-matched healthy individuals were enrolled in the study. A flowcytometric method was applied in the measurement of CD4+CD25+ T cells. The results showed that patients with SLE had statistically lower levels of CD4+CD25+ T cells than did normal controls, when expressed as either percentages of peripheral blood mononuclear cells (PBMCs) (mean ± SD, 8.49 ± 6.36 versus 11.11 ± 4.58%, P 〈 0.05) or absolute cell numbers (98.77 ± 97.52 versus 213.93 ± 104.52 cells/mm3, P 〈 0.05). In terms of CD25brightCD4+ T cells, defined as having a fluorescence intensity of CD25 expression exceeding 100, SLE patients still had significantly lower levels than did normal controls expressed as percentages of PBMCs (1.76 ± 1.32 versus 3.73 ± 1.30%, P 〈 0.05). No significant differences could be found between RA patients and normal controls. The overwhelming majority of CD4+CD25+ T cells belonged to CD45RO+ cells and most did not express the CD69 molecule. Although decreased CD4+CD25+ T cells were found in SLE patients, we failed to find a significant correlation between the levels of CD4+CD25+ T cells and disease activities of SLE. To the best of our knowledge, this is the first study to demonstrate that patients with SLE had decreased CD4+CD25+ T cells. However, the exact role of the decreased CD4+CD25+ T cells in the pathogenesis of SLE remains to be elucidated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0300-9475.2004.01370.x
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  • 3
    Electronic Resource
    Electronic Resource
    T and IgA B Lymphocytes of the Pharyngeal and Palatine Tonsils: Differential Expression of Adhesion Molecules and Chemokines (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd
    Scandinavian journal of immunology 60 (2004), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The pharyngeal (Ph) and palatine (Pa) tonsils, although located in different regions of the upper aero-digestive tract (UADT), are thought to protect the respiratory tract similarly against infections by inducing and disseminating T and surface IgA+ (sIgA+) B cells. We investigated the factors controlling the migratory properties of T and sIgA+ B lymphocytes in the UADT of pigs by comparing the expression of vascular addressins, homing receptors and chemokine transcripts in Ph/Pa tonsils, Peyer's patches (PP) and their draining lymph nodes (LN). The vascular addressin PNAd was detected on high endothelial venules in both tonsils, whereas mucosal addressin cell adhesion molecule-1, otherwise present in PP and mesenteric LN, was not detected. More importantly, the vascular cell adhesion molecule-1 (VCAM-1) addressin was present in Ph tonsil and LN but neither in Pa tonsil nor in PP vascular cells, whereas both T and sIgA+ B lymphocytes displayed similar levels of α4β1high integrin, the ligand of VCAM-1. Analysis of transcript levels for several lymphoid (CCL19, CXCL12 and CCL21) and epithelial chemokines also demonstrated opposite chemokine mRNA ratios for Ph tonsil (CCL28 〉 CCL25) and PP, with Pa tonsil expressing very low levels of CCL28. Collectively, these data indicate that the differential compartmentalization of sIgA+ lymphocytes between Pa and Ph tonsils may partly result from the differential expression of VCAM-1 and CCL28. They also suggest that tonsillar addressins and epithelial chemokines, rather than the cells intravasating it, control the regionalization of sIgA+ lymphocytes in the UADT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0300-9475.2004.01479.x
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  • 4
    Electronic Resource
    Electronic Resource
    Polymorphism of the immune-braking gene CTLA-4 (+49) involved in gender discrepancy of serum total IgE levels and allergic diseases (2004)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 34 (2004), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background A variety of genes are related to allergic disorders in different ethnic populations. The genetic basis for the gender discrepancy of allergic diseases remains to be determined.Objective This study was conducted to investigate whether IL-4 promoter (−590 C/T) and cytotoxic T lymphocyte antigen 4 (CTLA-4) (+49 A/G) polymorphisms were correlated with a gender discrepancy of total IgE levels and allergic diseases in a Chinese population.Methods A total of 1333 participants aged 19–49 years were enrolled in this study. Allergic diseases were recognized by the presence of asthma, rhinitis or atopic dermatitis in conjunction with detectable specific IgE in the blood. Polymorphisms of IL-4 promoter (−590) and CTLA-4 (+49) were determined by restriction fragment length polymorphism.Results Males or females with allergic diseases had higher total IgE levels than those without (P=0.000). Females with the A/A genotype in the CTLA-4 (+49) position had significantly higher total IgE levels than those with A/G, and those with the G/G genotype had the lowest IgE levels (154.9 vs. 107.1 vs. 79.8 KU/L; mean log values: 1.79 vs. 1.65 vs. 1.54, P〈 0.001). However, males with different genotypes in the CTLA-4 (+49) position exhibited no difference in the total IgE levels. Females with allergic rhinitis had a significantly higher frequency of the A/A genotype in the CTLA-4 (+49) polymorphism than those without atopic diseases (P=0.016). In contrast, males with and without allergic disorders exhibited no significant difference in the CTLA-4 (+49) polymorphisms (P〉0.05). The IL-4 promoter (–590) polymorphisms, however, had no correlation with the total IgE levels or allergic diseases in either females or males.Conclusion In females only, the CTLA-4 (+49), but not the IL-4 promoter (−590), polymorphism was significantly associated with elevation of total IgE levels and allergic rhinitis. Here, we have, for the first time, demonstrated a gender-linked genetic relationship with allergic disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.2004.01776.x
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  • 5
    Electronic Resource
    Electronic Resource
    The effect of retainer thickness on posterior resin-banded prostheses: a finite element study (2004)
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 31 (2004), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: summary  According to its design concept, a resin-bonded prosthesis, compared with the conventional fixed partial denture, is a weak and unstable structure. Therefore, a resin-bonded prosthesis induces a higher failure rate, especially in the posterior region. Recently, adhesion agents have been profoundly improved. However, the design guidelines of posterior resin-bonded prostheses (RBP) have seldom been evaluated from a biomechanical perspective. The objective of this study was to investigate the biomechanical effects of the retainer thickness on posterior RBP using the finite element method. A solid model of a posterior mandibular resin-bonded prosthesis, which employed the second molar and second premolar as the abutment teeth, was constructed and meshed with various retainer thickness (ranging from 0·2 to 1·0 mm). Horizontal and vertical loadings of 200 N were applied respectively at the central fossa of the pontic to examine the stress level at the interface between the retainer and abutment teeth. All exterior nodes in the root, below the cementoenamel junction were fixed as the boundary condition. The results showed that horizontal loading would induce higher interfacial stresses than the vertical loading which indicated that the horizontal component of the occlusal force plays a more important role in evaluating the debonding phenomenon. Further, the peak interfacial stresses increased as the retainer thickness decreased and, based on the fitted relation between retainer thickness and interfacial stresses, a 0·4 mm retainer thickness was suggested as the minimum required to prevent severe interfacial stresses increasing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2842.2004.01346.x
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  • 6
    Electronic Resource
    Electronic Resource
    Loss of Fhit protein in carcinoma of primary and secondary müllerian systems (2004)
    Oxford, UK : Blackwell Science Ltd
    Histopathology 44 (2004), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2559.2004.01747.x
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  • 7
    Electronic Resource
    Electronic Resource
    The use of bispectral index during a cardiopulmonary arrest: a potential predictor of cerebral perfusion (2004)
    Oxford, UK : Blackwell Science Ltd
    Anaesthesia 59 (2004), S. 0 
    Details
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A 62-year-old male underwent off-pump coronary artery grafting surgery while cerebral function was monitored with bispectral index (BIS). The BIS monitoring was continued into the immediate postoperative period, during which time the patient experienced a cardiopulmonary arrest. The changes in the BIS values helped the resuscitating team in assessing the cerebral response to the cardiopulmonary resuscitation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2044.2004.03736.x
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  • 8
    Electronic Resource
    Electronic Resource
    Study on Preprocessing Methods for Color 3D Point Cloud (2004)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 471-472 (Dec. 2004), p. 716-721 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper provides a preprocessing flow for color three-dimension (3D) point cloud according to the characteristics of laser scanning data. The preprocessing methods and their functions are introduced in detail. Automatic system decision and manual polygon selection methods are applied to eliminate unwanted and noise data successfully, which possibly make improper color models reconstructed. A data reduction method is presented based on Grid reduction method considering color-boundary preservation. It can effectively avoid shape and color distortion in modelreconstruction. Several experimental results are presented to indicate the feasibility and reliability of these methods, which can be broadly applied in various fields of products design, antiques and art wares digitization, etc
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/09/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.471-472.716.pdf
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  • 9
    Electronic Resource
    Electronic Resource
    The Superplastic Forming Technology of Ti-6Al-4V Titanium Alloy Bellows (2004)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 447-448 (Feb. 2004), p. 247-252 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/07/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.447-448.247.pdf
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  • 10
    Electronic Resource
    Electronic Resource
    Formation of silver particles and silver oxide plume nanocomposites upon pulsed-laser induced liquid-solid interface reaction (2004)
    Springer
    The European physical journal 41 (2004), S. 479-483 
    Details
    ISSN: 1434-6036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. Nanodendrites consisting of silver and silver oxide are grown upon a pulsed-laser induced reaction at the interface between the solid target and silver nitrate solution. By using a high-resolution transmission electron microscope (HRTEM) equipped with an energy dispersive X-ray spectrometer (EDS) and selected area diffraction (SAD), the fabricated nanopatterns are identified to be a composite structure that consisting of silver nanoparticles and silver oxide nanoplumes with polycrystalline structure. In detail, these silver nanocrystals are trunks of the nanodendrite, and their size is in the range of 30 to 50 nm. The silver oxide nanoplumes are branches of the nanodendrite, and their width and length are in the ranges of 20 to 50 nm and 30 to 100 nm, respectively. Additionally, we suggested a vapor-liquid mechanism for the formation of the nanopatterns using a pulsed-laser induced liquid-solid interface reaction, in which both silver clusters in vapor and silver ions in solution are simultaneously involved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1140/epjb/e2004-00341-4
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