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  • 2005-2009
  • 2000-2004  (2)
  • HL60 cell line  (1)
  • Key words:POU, Sox, Pax, ganglia, mollusk, lophotrochozoan.  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Marine biotechnology 2 (2000), S. 545-557 
    ISSN: 1436-2236
    Keywords: Key words:POU, Sox, Pax, ganglia, mollusk, lophotrochozoan.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract: In gastropod mollusks, neuroendocrine cells in the anterior ganglia have been shown to regulate growth and reproduction. As a first step toward understanding the molecular mechanisms underlying the regulation of these physiological processes in the tropical abalone Haliotis asinina, we have identified sets of POU, Sox, and Pax transcription factor genes that are expressed in these ganglia. Using highly degenerate oligonucleotide primers designed to anneal to conserved codons in each of these gene families, we have amplified by reverse transcriptase polymerase chain reaction 2 POU genes (HasPOU-III and HasPOU-IV), 2 Sox genes (HasSox-B and HasSox-C), and two Pax genes (HasPax-258 and HasPax-6). Analyses with gene-specific primers indicated that the 6 genes are expressed in the cerebral and pleuropedal ganglia of both reproductively active and spent adults, in a number of sensory structures, and in a subset of other adult tissues.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-4994
    Keywords: Fluorogenic probes ; microspectrofluorometry ; HL60 cell line ; oxidative stress ; thiol status
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Development of microspectrofluorometric methods using specific fluorogenic probes has provided precious help in studying in situ oxidative stress and cellular protective systems. The aim of this study was to determine ROS production concomitantly with a modification of the intracellular thiol pool after applying an oxidative stress to a nonadherent cell model represented by the HL60 cell line. The dichlorodihydrofluorescein diacetate (H2DCFDA) probe assessed the kinetic production of ROS by cells submitted to the chemical oxidant t-butylhydroperoxide with a high signal/noise ratio. The probe sensitivity permitted us to detect endogenous ROS production in HL60 cells and the protective effect of N-acetyl cysteine against ROS. The chloromethylfluorescein diacetate probe (CMFDA) permitted us to evaluate the thiol depleting effect of N-ethyl maleimide. Complete thiol depletion was associated with a moderate increase in ROS production. The cell viability was determined with calcein-AM, which gave results similar to those with the tetrazolium dye. This probe was not affected by intracellular pH and did not required an extraction step, unlike tetrazolium dye. In conclusion, cell-permeant fluorogenic probes are useful and sensitive tools to determine in situ ROS production concomitantly with consecutive change in the thiol system in a living and non-adherent cell model.
    Type of Medium: Electronic Resource
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