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  • 11
    Electronic Resource
    Electronic Resource
    Olive allergen-specific IgE responses in patients with Olea europaea pollinosis (2002)
    Oxford, UK : Blackwell Science Ltd
    Allergy 57 (2002), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Olive tree (Olea europaea) pollen is an important cause of pollinosis in the countries of the Mediterranean area.Objective This work aimed to study the IgE-binding frequency of Ole e 1, Ole e 2, Ole e 3, Ole e 6 and Ole e 7 from O. europaea pollen in a large population of olive pollen-allergic patients.Methods We studied: 119 consecutive patients with seasonal rhinitis and/or asthma and a positive skin prick test to O. europaea pollen extract; 10 atopic patients without history of pollinosis and a negative skin prick test to O. europaea; and 10 healthy controls. Allergens were purified from O. europaea pollen extract by reverse phase HPLC and characterized by N-terminal amino acid sequencing, MALDI analysis, and specific IgE immunodetection. Skin prick tests and ELISA titration against above mentioned purified olive pollen allergens were performed in all pollinic patients and controls.Results One-hundred and seven (90.7%) patients had a positive skin response to Ole e 1; 88 (74.6%) reacted to Ole e 2; 57 (47.9%) reacted to both Ole e 6 and Ole e 7; and 43 (37.8%) reacted to Ole e 3. The allergenic activity determined by ELISA to Ole e 1 was found in 84%; to Ole e 2 in 61.3%; to Ole e 3 in 31.9%; to Ole e 6 in 39.4%; and to Ole e 7 in 41.2% of patients. All patients had positive skin responses to at least one of the allergens tested, However, a combination of Ole e 1 and Ole e 2 together with a minor allergen Ole e 6 or Ole e 7, disclosed the same diagnostic value that was obtained with the use of crude olive pollen extract. The nonatopic and atopic control subjects did not react to any purified allergens on the skin prick test.Conclusions These results indicate that Ole e 1 and Ole e 2 are major allergens in patients with O. europaea pollinosis in our population. A combination of a few olive pollen allergens can substitute the crude extract for in vivo as well as in vitro diagnostic purposes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2002.057s71047.x
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  • 12
    Electronic Resource
    Electronic Resource
    Nanotubes Obtained from Mono and Bimetallic Carbides Produced by Mechanical Alloying (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 539-543 (Mar. 2007), p. 2731-2736 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The main purpose of this work was to produce nanotubes using a two step method:mechanical alloying and heat treatment. Mechanical Alloying (MA) was used to prepare themonometallic Co3C carnide and the bimetallic C-25%at Ni-25%at Mo carbide with Ti impuriritiesby milling of pure elemental powders of cobalt, molibdenum, titanium and carbon, in a high-energyrotatory mill under an Ar atmosphere. The nanocrystalline carbides were used to produce metalfilled nanotubes and nanoparticles, by means of precipitation after heating for 15 minutes at 800°C.Microstructural characterisation of the as-milled and heat-treated powders was performed usingTransmisssion Electron Microscopy (TEM) techniques. It was possible to obtain filled nanotubes,carbide nanorods, and observe a nucleation phenomena inside carbon cavities
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/15/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.539-543.2731.pdf
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  • 13
    Electronic Resource
    Electronic Resource
    The Specific Force of Single Intact Extensor Digitorum Longus and Soleus Mouse Muscle Fibers Declines with Aging (2000)
    Springer
    The journal of membrane biology 178 (2000), S. 175-183 
    Details
    ISSN: 1432-1424
    Keywords: Key words: Aging — Skeletal muscle — Single fiber —Soleus—Extensor digitorum longus—Flexor digitorum brevis— Excitation-contraction coupling — Sarcopenia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. In the present study we measured, for the first time, the isometric specific force (SF, force normalized to cross sectional area) generated by single intact fibers from fast- (extensor digitorum longus, EDL) and slow-twitch (soleus) muscles from young adult (2–6), middle-aged (12–14) and old (20–24 month-old) mice. SF has also been measured in single intact flexor digitorum brevis fibers from young mice. Muscle fibers have been classified into fast- or slow-twitch based on the contraction kinetics. Maximum SF recorded in EDL and soleus fibers from young and middle-aged mice did not differ significantly. A significant age-dependent decline in maximum SF was recorded in EDL and soleus fibers from young or middle-aged to old mice. The SF was 377 ± 18, 417 ± 20 and 279 ± 18 kPa for EDL fibers from young, middle-aged and old mice, respectively and 397 ± 17, 405 ± 24 and 320 ± 33 kPa for soleus fibers from age-matched mice, respectively. The frequency needed to elicit maximum force in EDL and soleus fibers from middle-aged to old mice did not differ significantly. In conclusion, the specific force developed by both fast and slow-twitch single intact muscle fibers declines with aging and more significantly in the former.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002320010025
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  • 14
    Electronic Resource
    Electronic Resource
    Transabdominal Surgical Approach in the Management of Renal Tumors Involving the Retrohepatic Inferior Vena Cava (2000)
    Springer
    Annals of vascular surgery 14 (2000), S. 436-443 
    Details
    ISSN: 1615-5947
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100169910083
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