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  • 2005-2009  (1)
  • 1995-1999  (3)
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  • 1
    Digitale Medien
    Digitale Medien
    Effects of temperature and continuous and interrupted wetness on the infection of pear leaves by conidia of Venturia nashicola (2005)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 54 (2005), S. 0 
    Details
    ISSN: 1365-3059
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Experiments were conducted to determine: (i) the effects of temperature and duration of continuous wet periods on the infection of pear seedlings by conidia of Venturia nashicola, the causal agent of pear scab; and (ii) the effects of the length and temperature of dry interrupting periods on the mortality of infecting conidia. Average number of scab lesions per leaf increased with increasing duration of wetness. Logistic models adequately described the change in the average number of scab lesions per leaf at 5, 10, 15, 20 and 25°C over the wetness duration. At 30°C, only a few lesions developed. Simple polynomial models satisfactorily described the relationship of the three logistic model parameters (maximum number of lesions, rate of appearance and the time to 50% of the maximum number of lesions) with temperature. The optimum temperature for infection was found to be approximately 20°C. The relationship between mortality and the length of a dry period interrupting an infection process can be satisfactorily described by an exponential model. The rate of mortality at 10, 16 and 22°C did not differ significantly, but was significantly less than that at 28°C.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-3059.2005.01207.x
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  • 2
    Digitale Medien
    Digitale Medien
    Benefit of transcription-coupled nucleotide excision repair for gene expression in u.v.-damaged Escherichia coli (1995)
    Osney Mead, Oxford OX2 0EL, UK : Blackwell Science Ltd
    Molecular microbiology 18 (1995), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Expression of the lactose operon upon induction by IPTG was studied with Escherichia coli B/r and K-12 strains as a function of exposure to ultraviolet light. Patterns of expression inactivation were compared in cells with wild-type UvrABC nucleotide excision repair, with transcription-coupled excision repair (TCR) specifically defective because of a defect at mfd, or with excision repair (ER) and TCR eliminated by defects at uvrA or uvrC. Sets of inactivation patterns were also determined for cells expressing the lactose operon via the ‘UV5’ promoter, an alternative to the wild-type promoter that eliminates dependence of expression on negative DNA supercoiling. The results demonstrated a major contribution by TCR to successful gene expression. Gene expression was more sensitive to u.v. inactivation when TCR was defective and similarly more sensitive when both ER and TCR were defective. Thus, TCR may be the only means of repairing transcription-blocking damage at active genes. Contrasting results with wild-type and UV5 promoters suggested that relaxed supercoiling might accompany repair and reduce expression even though a template lesion is removed. A test of mismatch repair defects on ultraviolet inactivation of gene expression found only limited interference with TCR as it benefits gene expression.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2958.1995.mmi_18040615.x
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  • 3
    Digitale Medien
    Digitale Medien
    Mutation frequency decline in Escherichia coli. I. Effects of defects in mismatch repair (1995)
    Springer
    Molecular genetics and genomics 249 (1995), S. 585-590 
    Details
    ISSN: 1617-4623
    Schlagwort(e): UV mutagenesis ; Excision repair ; Mismatch repair
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Mutation frequency decline (MFD) in Escherichia coli was examined for effects associated with genetic defects in mismatch repair. The kinetics of MFD are slower when the B/r strain WU3610 carries the mutation mutS201::Tn5 or mutL::Tn10, both of which affect mismatch repair. Similar slow kinetics are produced by mutH34 but not by mutH471::Tn5; the latter has no apparent effect. Strain WU3610-45 (mfd-1) produces the slower kinetics if transcription is inhibited during the post-UV incubation, although it produces no decline in normal circumstances. The slower kinetics are therefore attributed to bulk excision repair that remains when rapid transcription-coupled repair (TCR) is eliminated by certain defects in mismatch repair. A model is proposed wherein mismatch repair defects are thought to slow the activity of TCR but, unlike an mfd defect, not to impede dissociation of stalled transcription complexes at lesions in the transcribed DNA strand.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00418027
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  • 4
    Digitale Medien
    Digitale Medien
    Mutation frequency decline in Escherichia coli. II. Kinetics support the involvement of transcription-coupled excision repair (1995)
    Springer
    Molecular genetics and genomics 249 (1995), S. 591-599 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Targeted mutagenesis ; Excision repair ; Cyclobutane pyrimidine dimers ; Cytosine deamination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Mutation frequency decline (MFD) in Escherichia coli was examined to demonstrate repair of targeting photoproducts during the post-UV incubation required in this process. Repair of mutation-targeting cyclobutane pyrimidine dimers (T 〈〉 C) was demonstrated when a correlation was established between the mutation frequency normally associated with these lesions and the rate of mutation production at these lesions by spontaneous deamination of cytosines and photoreversal in ung-defective cells. An incubation producing a decline in mutation frequency, i.e., MFD, also produces lower rates of mutation increase via the deamination mechanism. Since the latter assay involves processes entirely within the post-UV incubation period, the lower rates are attributed to rapid transcription-coupled nucleotide excision repair (TCR) that reduces the number of relevant T 〈〉 C dimers during this period. Rediscovery of the neglected fact that MFD can be stimulated by post-UV incubation in buffer alone is part of the analysis. Results presented here and a variety of others are discussed to support a model of MFD as a particular example of TCR: effective repair of photoproducts in the transcribed DNA strand that target glutamine tRNA suppressor mutations occurs during the appropriate post-UV incubation and is responsible for MFD.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00418028
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