ISSN:
1432-203X
Keywords:
Embryogenic cultures
;
Cryopreservation
;
Picea glauca engelmanni complex
;
Somaclonal variation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Embryogenic cultures of interior spruce derived from 12 full-sib families were subjected to cryopreservation, with a 97 % success rate for 357 genotypes. Analyses suggested that cryotolerance was not related to family ranking (height increment), embryogenic potential or culture dispersability in suspension, and long-term storage in or above liquid nitrogen did not affect regenerative potential. By contrast, differences in cryotolerance among cell lines appeared to be prevalent in certain families. Analysis with a DNA fingerprinting probe used for clonal identification demonstrated no evidence of somaclonal variation as a result of cryopreservation. The results of this work indicate the applicability of cryopreservation as a long-term storage strategy for spruce embryogenic cultures from a wide genetic background.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00234514
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