ISSN:
1435-1463
Keywords:
Familial amyloidotic polyneuropathy
;
aorta
;
alpha1-adrenergic receptor
;
3H-bunazosin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary To assess the pathophysiology of the sympathetic nervous system in familial amyloidotic polyneuropathy (FAP), we used3H-bunazosin to identify and characterize the alpha1-adrenergic receptor in human aortic membranes. The binding of3H-bunazosin was rapid, readily reversible, stereospecific, and saturable. The Scatchard analysis described a single class of binding sites with a dissociation constant (KD) of 0.370±0.035nM and a maximal binding capacity (Bmax) of 11.8±1.30 fmol/mg protein in control patients. Competition analysis demonstrated the alpha1-adrenergic specificity of the3H-bunazosin binding sites in human aortic membranes. The KD and Bmax of3H-bunazosin binding in four FAP patients was 0.274±0.052 nM and 7.79±0.15 fmol/mg protein, respectively; these values did not differ significantly from those in 14 control patients. An increase in Bmax or affinity of alpha1-adrenergic receptors may not be the cause for denervation supersensitivity in FAP.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01244619
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