Bibliothek

Notizen: SYNOPSIS. A technic is described for the quantitative assay of paramylum content of euglenoid flagellates. The method relies on the alkaline solubility of paramylum followed by treatment with the anthrone reagent. The intensity of the color developed by paramylum is about 14% greater than that developed by an equivalent amount of glucose. The method is sensitive down to about 10 μg.

Notizen: SYNOPSIS. Light-dependent incorporation of acetate occurs in an obligate phototrophic strain of Euglena gracilis (strain L). Assimilation is into all major biochemical fractions. Acetate does not induce operation of the glyoxylate by-pass as it does in heterotrophic strains; neither does it stimulate oxygen consumption. Acetate will not replace CO2 in phototrophic growth. A number of carbon sources tested would not support growth in the dark, and glucose was not incorporated either in the light or the dark.

Notizen: A 32-year-old African American male presented with a five year history of multiple verrucous plaques in a Blaschkoid arrangement on the left leg. Biopsies demonstrated squamous cell carcinoma in-situ. The patient’s medical history did not reveal any heritable or exposure related risk factors. The patient was treated with radiotherapy. Four years later, he presented with a subcutaneous nodule within a prior site of radiation. MRI showed a 2.0 × 1.2 cm enhancing nodule in the subcutis. On biopsy, an ill-defined spindle cell proliferation was present in the dermis and subcutis. Marked nuclear pleomorphism and numerous multinucleated cells embedded in a myxoid stroma were noted. Spindle cells stained for CD68. Cytokeratin and S-100 were negative. A diagnosis of Malignant Fibrous Histiocytoma was rendered. Surgical excision was undertaken. A dominant lesion of MFH was identified, as well as multiple, clinically undetectable foci of early sarcoma. This is an unusual case of multifocal post-radiation sarcoma in an African American patient with squamous cell carcinoma in-situ. While sarcoma is a known risk factor following radiotherapy, the course of this patient would suggest caution in applying radiation in similar clinical settings, as well as long-term clinical follow-up for early detection and treatment of post-radiation sarcoma.

Notizen: Four intermediate-heading perennial ryegrass (Lolium perenne L.) varieties, which in previous studies had been associated with high- or low-intake characteristics when swards containing them had been continuously stocked with sheep, were sown as monocultures. They were rotationally grazed, using 1-d paddocks, with core groups of four yearling Simmental × Holstein beef heifers in 2002 and 2003 and ingestive and ruminative behaviour, and sward factors, were measured. There were two diploid (Belramo and Glen) and one tetraploid (Rosalin) perennial ryegrass varieties and one tetraploid hybrid (Lolium × boucheanum Kunth) (AberExcel) variety. Intake rate (IR) was significantly higher in August 2003 for heifers grazing Glen than those grazing Belramo [27·5 vs. 20·6 g dry matter (DM) min−1; P = 0·019], but there were no significant differences between varieties in two other measurement periods. This is in contrast to previous results with sheep when IR were significantly higher for Glen than Belramo and for AberExcel than Rosalin. Total jaw movement rates during grazing were significantly higher for heifers on the tetraploid swards than those on the diploid swards (87·7 vs. 83·6 jaw movements min−1; P = 0·023) in September 2002. Ruminating time was significantly lower for heifers on the tetraploid swards than those on the diploid swards (453 vs. 519 min 24 h−1; P = 0·012) in July 2002. Digestibility of grass snips was significantly higher on the tetraploid than the diploid swards [697 vs. 680 g digestible organic matter (DOM) kg−1 DM; P = 0·042] in September 2003 and, within diploids, was significantly higher for Glen than Belramo (696 vs. 663 g DOM kg−1 DM; P = 0·014). There were significant differences in sheath tube and leaf lengths and in the population density of tillers between and within ploidies, which might have been expected to have influenced intake characteristics, but this was not generally found under rotational grazing with cattle. In order to separate the effects of defoliation interval from those of grazing style of the different ruminant species, it is suggested that grass variety evaluations using continuously stocked cattle swards are required.