ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The purpose of this study was to evaluate the cytotoxicity of nanometer magneticcomposite via being cultured with osteoblast-like cells (MG-63). The osteoblast-like cell (MG-63)was utilized as the seed cell. The cells were cultured on the surface of nanometer magnetic composite.After union culturing, on the day 1, 3, 5, 7, the cytotoxicity of nanometer magnetic composite wasevaluated by MTT method and the cell morphology was observed by SEM. Meanwhile, on the day 4,7, 10, the alkaline phosphatase (ALP) activity was tested respectively. These results demonstrated thatthe typical cell morphology could be observed when the osteoblast-like cells were cultured onnanometer magnetic composite substrate in vitro. Cell proliferation and ALP activity became higheras the prolongation of cultivate time in the group of nanometer magnetic composite. The studyshowed that nanometer magnetic composite had a little inhibition to cell proliferation and ALPactivity in vitro cell culture, as compared with the chitin and PLA fiber substrate. So the feasibility ofnanometer magnetic composite as scaffold for bone tissue engineering should be studied further
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.1103.pdf
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