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  • 11
    Electronic Resource
    Electronic Resource
    Gutartiger Tumor des AV-Knotens (Mesotheliom) und plötzlicher Tod (1998)
    Springer
    Rechtsmedizin 8 (1998), S. 102-104 
    Details
    ISSN: 1434-5196
    Keywords: Key words Heart ; Conduction system ; Mesothelioma ; of av-node ; Schlüsselwörter Herz ; Erregungsleitungssystem ; Gutartiger Tumor ; Mesotheliom
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Bericht über 2 Fälle mit einem gutartigen Tumor des AV-Knotens (sog. Mesotheliom). Im einen Fall dürfte der Tumor für eine langjährig bekannte Erregungsleitungsstörung (AV-Block) und für den plötzlichen Todeseintritt verantwortlich sein.
    Notes: Abstract A report on two cases with benign tumors of the atrioventricular node (so-called mesothelioma of the av-node or endodermal inclusions of the heart). In one of the cases the tumor could be the cause of the known electrophysiological disturbance of the conduction system (av-block) and of the sudden and unexpected death.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001940050039
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  • 12
    Electronic Resource
    Electronic Resource
    The substitution chemistry of RuCp* (temeda)Cl (1997)
    Springer
    Monatshefte für Chemie 128 (1997), S. 1189-1199 
    Details
    ISSN: 1434-4475
    Keywords: Ruthenium half-sandwich complexes ; Tetramethylethylenediamine ; Ruthenacycles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Chloridabspaltung von RuCp*(tmeda)Cl (1,tmeda=Me2NCH2CH2NMe2) mittels NaBPh4 in CH2Cl2 führt zur Bildung des Halbsandwich-Komplexes RuCp*(η6-C6H5BPh3) (2), während in Gegenwart von CH3CN oder CO die beiden kationischen Verbindungen [RuCp*(tmeda)(CH3CN)]+ (3) und [RuCp*(tmeda)(CO)]+ (5) entstehen. In CH3CN als Lösungsmittel wird sogartmeda unter Bildung von [RuCp*(CH3CN)3]+ (4) verdrängt. Komplex1 reagiert sehr leicht mit terminalen Alkinen HC≡CR, wobei die Produkte stark von der Natur des SubstituentenR (Ph, SiMe3,n-Bu, COOEt) abhängen. Im Fall vonR=Ph entsteht der Ruthenacyclopentatrien-Komplex RuCp*(σσ′-C4Ph2H2)Cl (6), mitR=SiMe3 der Cyclobutadien-Komplex Ru(Cp*)(η4-C4H2(1,2-SiMe3)2)Cl (7), und im Fall vonR=n-Bu und COOEt bilden sich die binuklearen Komplexe (Cp*)RuCl2(η2:η4-μ2-C4H2(1,3-R)2)Ru(Cp*) (8,9). Überdies reagiert1 mit Maleinsäurediethylester in Gegenwart von LiCl zum neuen anionischen Komplex Li[Ru(Cp*) (η2-C2H2(COOEt)2)Cl2] (10). Von2,3,4,7 und10 wurden die Kristallstrukturen bestimmt.
    Notes: Summary Halide abstraction from RuCp*(tmeda)Cl (1,tmeda=Me2NCH2CH2NMe2) with NaBPh4 in CH2Cl2 leads to the formation of the sandwich complex RuCp*(η6-C6H5BPh3) (2). In the presence of CH3CN (1 equiv.) and CO, however, the cationic complexes [RuCp*(tmeda)(CH3CN)]+ (3) and [RuCp*(temeda)(CO)]+ (5) are obtained. In CH3CN,tmeda is also replaced giving [RuCp*(CH3CN)3]+ (4). Complex1 reacts readily with terminal acetylenes HC≡CR, the products depending on the nature ofR (Ph, SiMe3,n-Bu, COOEt). Thus, withR=Ph the ruthenacyclopentatriene complex RuCp*(σ,σ′-C4Ph2H2)Cl (6), withR=SiMe3 the cyclobutadiene complex Ru(Cp*)(σ4-C4H2(1,2-SiMe3)2)Cl (7), and withR=n-Bu and COOEt the binuclear complexes (Cp*)RuCl2(η2:η4-μ2-C4H2(1,3-R)2)Ru(Cp*) (8,9) are obtained. Furthermore, with diethyl maleate in the presence of 1 equiv. of LiCl,1 transforms into the new anionic complex Li[Ru(Cp*) (η2-C2H2(COOEt)2)Cl2] (10). X-ray structures of2,3,4,7, and10 are included.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00807250
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  • 13
    Electronic Resource
    Electronic Resource
    Synthesis and X-ray structures of some mono- and binuclear ruthenium complexes with bisphosphine ligands (1997)
    Springer
    Monatshefte für Chemie 128 (1997), S. 533-540 
    Details
    ISSN: 1434-4475
    Keywords: Ruthenium ; Binuclear complexes ; X-Ray analysis ; Oxidation ; Bisphosphines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die Komplexe {RuCp*(μ-Cl)}2(μ-dppm) (1) und {RuCp*(μ-Cl2(μ-dppe) (3) wurden durch Umsetzung von [RuCp*(μ3-Cl)]4 mitdppm bzw.dppe dargestellt.1 wird durch AgCF3SO3 zum zweikernigen Komplex [{RuCp*(μ-Cl)}2(μ-dppm)](SO3CF3)2 (2) oxidiert, welcher eine Ru-Ru-Metallbindung aufweist. Unter den gleiche Reaktionsbedingungen zersetzt sich3 zu undefinierten Produkten. Analog zu1 reagiert RuCp* (dmpe)Cl mit AgCF3SO3 zum Ru(III)-Komplex [Ru(Cp*)(dmpe)Cl](SO3CF3) (4) wobei es zu keiner Chloridabspaltung kommt. Von2,3, und4 wurden die Kristallstrukturen bestimmt.
    Notes: Summary The dinuclear complexes {RuCp*(μ-Cl)}2(μ-dppm) (1) and {RuCp*(μ-Cl)}2 (μ-dppe) (3) are obtained by reacting [RuCp*(μ3-Cl)]4 withdppm, anddppe, respectively.1 is readily oxidized with AgCF3SO3, instead of chloride abstraction, to afford the dinuclear complex [{RuCp*(μ-Cl)}2(μ-dppm)](SO3CF3)2 (2) with two metal centers connected by a single Ru-Ru bond. Under the same conditions,3 decomposes to several intractable materials. Similarly to1, RuCp* (dmpe)Cl reacts with AgCF3SO3 to afford the Ru(III) complex [RuCp*(dmpe)Cl](SO3CF3) (4) without no halide abstraction. The crystal structures of2,3, and4 are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00807583
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  • 14
    Electronic Resource
    Electronic Resource
    Genomic organization and chromosomal mapping of mouse nuclear factor kappa B 2 (NFKB2) (1999)
    Springer
    Immunogenetics 49 (1999), S. 743-750 
    Details
    ISSN: 1432-1211
    Keywords: Key words NFKB2 ; p52 ; p100
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  NFKB2 is a member of the NFKB/Rel gene family, which is known to be a pivotal regulator of the acute phase and immune responses. NF-κB2 is initially synthesized as a ∼100 000 M r protein which needs to be processed in order to bind DNA, either as homodimer or as heterodimer with other members of the NF-κB/Rel family. The unprocessed form of NF-κB2 acts as an IκB-like protein. Therefore, NF-κB2 has a dual function. In this report we describe the genomic structure, expression pattern, and chromosomal localization of mouse NFKB2. Genomic clones were isolated, which span the entire gene of approximately 8.5 kilobases (kb) including 1.5 kb of the promoter region. Comparison to its human and avian homologues revealed a strong evolutionary conservation of the gene structure including the exon/intron borders, sequence, and position of the nuclear localization signal, the glycine-hinge region, and the ankyrin repeats. By fluorescence in situ hybridization, mouse NFKB2 was mapped to Chromosome (Chr) MMU 19C3-D2, which is homologous to human Chr 10q24, at which position the human NFKB2 was previously located. NFKB2 is ubiquitously expressed, highest in lymph nodes and thymus, underlining its role in the immune function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050548
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  • 15
    Electronic Resource
    Electronic Resource
    Über einige Sulfidfluoride der Seltenen Erden (1965)
    Springer
    Naturwissenschaften 52 (1965), S. 475-475 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00626236
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  • 16
    Electronic Resource
    Electronic Resource
    Kongenitale myotone Dystrophie (1997)
    Springer
    Monatsschrift Kinderheilkunde 145 (1997), S. 588-592 
    Details
    ISSN: 1433-0474
    Keywords: Schlüsselwörter Kongenitale myotone Dystrophie ; Curshmann-Steinert-Batten-Syndrom ; CTG-Trinukleotid-Sequenzwiederholung ; Dystrophia myotonica ; Facies myopathica ; Key words Congenital myotonia dystrophy ; Curshmann-Steinert-Batten-Syndrom ; CTG-Trinukleotid sequence repetition ; Distrophy myotonica ; Facies myopathica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The subject of this report is a newborn female, suffering from hypotonia and breathing difficulties, delivered in the 37 week with the help of forceps. Because of the nature of her symptons and those of the mother, which included Facies myopathica and Myotonia, the possibility of the expansion of a CTG-Trinukleotidsequence in the area of Chromosom 19 was explored. For this purpose a technique, developed in 1992, for the identification of molecular genetic characteristics was used. Instead of the 5 to 27 copies of the CTG sequence normally found in the population, the sick child had 1000 and the mother more than 700. EMG, in which a classical relases of myotones (Fall-Fight-Bomberscream) were found, confirmed the neurological diagnosis. Discussion: In the course of time the newborn child showed the classical problems of hyptotonia, such as respiratory difficulties, eating disorders leading to a loss of weight, meteroism, and after another stay in hospital, symptoms of Ileus.
    Notes: Zusammenfassung Es wird über ein in der 37. SSW durch Forzeps geborenes Mädchen berichtet, das durch Hypotonie und Ateminsuffizienz auffiel. Diese Symptome, ebenso wie die Facies myopathica und die Myotonie der Hand der Mutter bei der Begrüßung, gaben den Hinweis zur Durchführung der seit 1992 möglichen molekulargenetischen Bestimmung der Expansion einer CTG-Trinukleotidsequenz im Bereich des Chromosoms 19. Statt der in der Normalbevölkerung 5–27 Kopien dieser CTG-Sequenz-Wiederholung fanden sich bei dem erkrankten Kind über 1000 und bei der Mutter über 700. Darüber hinaus konnte durch eine neurologische Untersuchung der Mutter die bisher unbekannte Diagnose bestätigt werden, wobei im EMG eine klassische myotone Entladung (Sturz-Kampf-Bomber-Geräusch) gefunden wurde. Diskussion: Beim Neugeborenen traten im weiteren Verlauf die klassischen Folgeprobleme der Hypotonie wie Respiratorbedürftigkeit und Ernährungsprobleme mit relativer Gewichtsabnahme, Meteorismus und bei einer erneuten stationären Aufnahme Ileussymptomatik auf.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001120050156
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  • 17
    Electronic Resource
    Electronic Resource
    Die kongenitale Tibiapseudarthrose (2000)
    Springer
    Der Orthopäde 29 (2000), S. 821-831 
    Details
    ISSN: 1433-0431
    Keywords: Schlüsselwörter Kongenitale Tibiapseudarthrose ; Ilizarov-Methode ; Simultane Korrektur von Pseudarthrose ; Achsenfehlstellung und Beinlängendifferenz ; Keywords Congenital pseudarthrosis of the tibia ; Circular frame technique ; Simultaneous correction of pseudarthrosis ; Length discrepancy and axial deformity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract Congenital pseudarthrosis of the tibia (CPT) is one of the most challenging conditions in paediatric orthopaedics. The history of CPT treatment ischaracterised by repeatedly ineffective surgical interventions with the inevitable outcome of severe disability. Most procedures failed to account for the complexity of the problem. The past two decades have witnessed more experience having been gained with the Ilizarov technique. This method provides a comprehensive approach to all aspects of CPT problem, allowing the surgeon to simultaneously address the problems of union as well as those of deformity, length discrepancy joint function, ankle valgus and weight bearing. It appears that the goal to achieve stable fusion before the child begins school is attainable. In the pre-pseudoarthrotic stage, bracing should be preferred to surgery until the patient is in an older age group. This study reports on the results of 19 patients: 15 have finished treatment, and 14 of them with a stable fusion at follow-up. The results were highly encouraging in reaching the ultimate goal of avoiding amputation and achieving a well-functioning limb.
    Notes: Zusammenfassung Die kongenitale Tibiapseudarthrose gehört zu den schwierigsten Krankheitsbildern in der Kinderorthopädie. Anamenstisch findet sich bei diesen Patienten meist eine Vielzahl ineffektiver, operativer Eingriffe und letztendlich auch ein funktionell unbefriedigendes Ergebnis in Form einer den Patienten schwer behindernden Deformität. Bei den meisten dieser Behandlungsverfahren wird der Komplexität des Problems kongenitale Tibiapseudarthrose nicht entsprechend Beachtung geschenkt. In den letzten Jahren wurden deshalb Verfahren entwickelt, die eine erfolgreiche Behandlung ermöglichen. Es handelt sich dabei um den vaskularisierten Fibulatransfer und die Ilizarov-Technik. Letzterer muss unserer Meinung nach deshalb der Vorzug gegeben werden, da sie dem behandelnden Orthopäden die Möglichkeit gibt simultan sowohl Pseudarthrose, als auch Achsendeformität, Beinlängendifferenz, und Sprunggelenkvalgus zu behandeln. Weiter hat sich gezeigt, dass allzu frühe operative Eingriffe ungünstig sind und dass es sinnvoll ist, primär eine konsequente konservative Therapie durchzuführen, um im Alter von 3–6 Jahren operativ eine stabile Fusion der Pseudarthrose mit Korrektur der Begleitdeformität zu erreichen. Die vorliegende Arbeit berichtet über eigene Ergebnisse bei 19 Patienten, von denen bei 15 die Behandlung abgeschlossen wurde, wobei bei 14 Patienten eine stabile Fusion zum Zeitpunkt der Nachuntersuchung erzielt werden konnte. Die Ergebnisse waren sowohl klinisch als auch funktionell äußerst zufriedenstellend und lassen den Schluss zu, dass eine erfolgreiche Behandlung der Tibiapseudarthrose heute möglich ist und eine Amputation daher als obsolet angesehen werden muss.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001320050532
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  • 18
    Electronic Resource
    Electronic Resource
    Antiapoptotic bcl-2 and bcl-xL in advanced malignant melanoma (2000)
    Springer
    Archives of dermatological research 292 (2000), S. 225-232 
    Details
    ISSN: 1432-069X
    Keywords: Key words Melanoma ; Melanoma-metastases ; Bcl-2 ; Bcl-x ; Bax
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Apoptosis is an important cofactor in the pathogenesis of a plethora of malignancies. However, little is known about modulation of the expression of bcl gene family in melanocytic tumors. To determine the role of bcl-2, bcl-x and bax in melanocytic tumors we investigated the differential expression of these genes via RT-PCR in tissue samples from human ¶benign nevi, primary melanomas and melanoma metastases in comparison with normal skin. Bcl-2 was strongly expressed in 14/16 metastases (87.5%), whereas only 7/13 primary melanomas (53%), 7/15 nevi (46%) and 7/16 normal tissue samples (43%) showed expression of bcl-2 (P 〈 0.05). There was a strong indication of a correlation between tumor thickness and bcl-2 expression in nodular malignant melanomas. Expression of bcl-x was found in 16/16 melanoma metastases (100%), 11/13 primary melanomas (84%), 12/15 nevi (80%) and 10/16 normal tissue samples (62%) (P 〈 0.05). Bcl-xL expression increased from primary melanoma to melanoma metastases, whereas bcl-xS showed a decreasing expression level during melanoma progression. No differences in bax expression were seen between melanoma metastases, primary melanoma, nevi and normal tissue. Immunohistochemical investigations of another 53 tissue samples showed similar results. Our results strongly indicate that bcl-2 and bcl-xL gene expression increases with progression of malignant melanoma. Bcl-2 and bcl-xL expression could reflect an increased malignant potential caused by an inhibition of apoptosis and growth advantage for metastatic melanoma cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004030050479
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  • 19
    Electronic Resource
    Electronic Resource
    Structural and biochemical properties of glycosylated and deglycosylated glucose oxidase from Penicillium amagasakiense (1997)
    Springer
    Applied microbiology and biotechnology 47 (1997), S. 502-507 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Glucose oxidase from Penicillium amagasakiense was purified to homogeneity by ion-exchange chromatography and deglycosylated with endoglycosidase H. On the basis of gas chromatography and sodium dodecyl sulphate/polyacrylamide gel electrophoretic (SDS-PAGE) analyses, the protein-bound high-mannose-type carbohydrate moiety corresponded to 13% of the molecular mass of glycosylated glucose oxidase. A total of six N-glycosylation sites per dimer were determined from the N-acetylglucosamine content. The enzymatically deglycosylated enzyme contained less than 5% of the original carbohydrate moiety. A molecular mass of 130 kDa (gel filtration) and 133 kDa (native PAGE) was determined for the dimer and 67 kDa (SDS-PAGE) for the monomer of the deglycosylated enzyme. The N-terminal sequence, which has not been published for glucose oxidase from P. amagasakiense to date and which showed less than 50% homology to the N terminus of glucose oxidase from Aspergillus niger, and the amino acid composition were not altered by the deglycosylation. Deglycosylation also did not affect the kinetics of glucose oxidation or the pH and temperature optima. It also did not increase the susceptibility of the enzyme to proteolytic degradation. However, deglycosylated glucose oxidase exhibited decreased pH and thermal stability. The thermal stability of both enzymes was shown to be dependent on the buffer concentration and was enhanced by certain additives, particularly 1 M (NH4)2SO4, which stabilised glucose oxidase 100- to 300-fold at 50 °C and pH 7–8, and 2 M KF, which stabilised the enzyme up to 36-fold at 60 °C and pH 6. In sodium acetate buffer, changes in pH (4–6) affected the affinity for glucose but had no effect on the V max of the reaction. In contrast, in TRIS buffer, pH 8, a 10-fold decrease in V max and a 2-fold decrease in K m were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050963
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  • 20
    Electronic Resource
    Electronic Resource
    High-level expression of the thermoalkalophilic lipase from Bacillus thermocatenulatus in Escherichia coli (1998)
    Springer
    Applied microbiology and biotechnology 49 (1998), S. 405-410 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An efficient expression system for the previously only weakly expressed thermophilic lipase BTL2 (Bacillus thermocatenulatus lipase 2) was developed for the production of large amounts of lipase in Escherichia coli. Therefore, the gene was subcloned in the pCYTEXP1 (pT1) expression vector downstream of the temperature-inducible λ promoter PL. Three different expression vectors were constructed: (i) pT1-BTL2 containing the mature lipase gene, (ii) pT1-preBTL2 containing the prelipase gene and (iii) pT1-OmpABTL2 containing the mature lipase gene fused to the signal peptide of the OmpA protein, the major outer membrane protein of E. coli. With pT1-BTL2 and pT1-preBTL2, comparable expression levels of 7000–9000 U/g cells were obtained independently of the E. coli host. In contrast, with E. coli JM105 harbouring pT1-OmpABTL2, 660 000 soluble lipase U/g cells was produced, whereas, with E. coli DH5α and BL321, production levels of 30 000 U/g cells were achieved. However, most of the lipase remained insoluble but active after cell breakage because of the unprocessed OmpA signal peptide. A simple cholate extraction followed by proteinase K cleavage and ultrafiltration allowed the isolation of 1.15 × 106 units of 90% pure mature lipase/wet cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051190
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