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  • 1
    Electronic Resource
    Electronic Resource
    Adsorption and abstraction of H(D) on clean and modified Al(111) and Ni(111) surfaces (1999)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 110 (1999), S. 1154-1162 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: The kinetics of adsorption and abstraction for the system H(D) on Al(111) and Ni(111) has been investigated using an efficient atomic beam source, thermal desorption spectroscopy, and multiplexed mass spectrometry. The initial sticking coefficient of atomic hydrogen on Ni(111) is 1.0 and independent of the angle of incidence. For H on Al(111) the initial sticking coefficient is 0.6 and increases with increasing angle of incidence (S(cursive-phi)∼cos−0.4 cursive-phi). On Al(111) preadsorbed oxygen leads to a decrease of the initial sticking coefficient for H down to 0.1; potassium on the other hand has no significant influence on the initial sticking probability. On both surfaces, Ni(111) and Al(111), abstraction of deuterium proceeds at a surface temperature of 150 K due to impinging H atoms (H+D→HD), with an initial abstraction coefficient of 0.20 on Al(111) and 0.12 on Ni(111). In the case of Al(111) this coefficient is nearly independent of the initial D coverage and therefore the abstraction reaction cannot be described by a simple Eley–Rideal process, but rather by a hot-precursor mechanism. In addition to abstraction there is also a small probability for the removal of an adsorbed species by a collision induced desorption process. Abstraction of deuterium by impinging H-atoms is strongly affected by modification of the Al(111) surface, either by oxygen or by potassium. Oxygen decreases the HD abstraction coefficient, probably by inhibiting the hot-precursor state of H on the surface. Potassium increases the HD abstraction rate, most likely by increasing the lifetime of atomic hydrogen in the precursor state. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.478157
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  • 2
    Electronic Resource
    Electronic Resource
    Interaction of atomic hydrogen with metal surfaces (1998)
    Springer
    Applied physics 67 (1998), S. 637-644 
    Details
    ISSN: 1432-0630
    Keywords: PACS: 68.10.Jy; 68.45.Da; 82.65.Pa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003390050835
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and partial characterization of the Kluyveromyces lactis homologue of SKP1 (2000)
    Springer
    Current genetics 38 (2000), S. 8-16 
    Details
    ISSN: 1432-0983
    Keywords: Key wordsSKP1 ; CTF13 ; Kinetochore ; Cell cycle ; Kluyveromyces lactis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The SKP1 gene of Kluyveromyces lactis was isolated as a suppressor of a lethal temperature-sensitive mutation in the Saccharomyces cerevisiae CTF13 gene (Chromosome Transmission Factor 13). KlSKP1 was localized at chromosome V, adjacent to KlPAS3. A similar arrangement of the two genes is present in S. cerevisiae. Disruption of the KlSKP1 gene was lethal, whereas overexpression of KlSKP1 lead to a decreased growth rate, to swollen and chain-forming cells with an increased DNA content, and to decreased plasmid stability. In both yeasts, promoter constructs lacking most of the purported binding sequence showed increased transcription levels of KlSKP1 in comparison to constructs with the entire promoter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940000128
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation and characterization of KlUBP2, a ubiquitin hydrolase gene of Kluyveromyces lactis that can suppress a ts-mutation in CBF2, a gene encoding a centromeric protein of Saccharomyces cerevisiae (2000)
    Springer
    Current genetics 38 (2000), S. 17-22 
    Details
    ISSN: 1432-0983
    Keywords: Key words Kluyveromyces lactis ; CBF2 ; UBP2 ; Ubiquitination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Kluyveromyces lactis UBP2 gene was isolated as a suppressor of a temperature-sensitive mutation in CBF2, a gene coding for a centromere-binding protein of Saccharomyces cerevisiae. The UBP genes are hydrolases than can cleave a ubiquitin moiety from a protein substrate. KlUBP2 is not essential for growth since a disruption of the KlUBP2 gene had little effect, except for a slight decrease in the growth rate. The stability of centromere-containing plasmids was not influenced either. In addition to KlUBP2, five S. cerevisiae genes involved in the ubiquitination pathway could suppress the ts-mutation in the CBF2 gene, namely UBA1, UBA2, UBP1, UBP2 and YUH1, although YUH1 was the only one that could do this like KlUBP2 from a single-copy plasmid. Surprisingly, these genes encode proteins with antagonistic activity as two, UBA1 and UBA2, are ubiquitin-activating enzymes whereas the other three are de-ubiquitinating hydrolases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940000129
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  • 5
    Electronic Resource
    Electronic Resource
    T-DNA from Agrobacterium tumefaciens as an efficient tool for gene targeting in Kluyveromyces lactis (1999)
    Springer
    Molecular genetics and genomics 261 (1999), S. 115-121 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsAgrobacterium tumefaciens ; Kluyveromyces lactis ; Gene targeting ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The soil bacterium Agrobacterium tumefaciens can transfer a part of its tumour-inducing (Ti) plasmid, the T-DNA, to plant cells. The virulence (vir) genes, also located on the Ti plasmid, encode proteins involved in the transport of T-DNA into the plant cell. Once in the plant nucleus, T-DNA is able to integrate into the plant genome by an illegitimate recombination mechanism. The host range of A. tumefaciens is not restricted to plant species. A. tumefaciens is also able to transfer T-DNA to the yeast Saccharomyces cerevisiae. In this paper we demonstrate transfer of T-DNA from A. tumefaciens to the yeast Kluyveromyces lactis. Furthermore, we found that T-DNA serves as an ideal substrate for gene targeting in K. lactis. We have studied the efficiency of gene targeting at the K. lactis TRP1 locus using either direct DNA transfer (electroporation) or T-DNA transfer from Agrobacterium. We found that gene targeting using T-DNA was at least ten times more efficient than using linear double-stranded DNA introduced by electroporation. Therefore, the outcome of gene targeting experiments in some organisms may depend strongly upon the DNA substrate used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050948
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  • 6
    Electronic Resource
    Electronic Resource
    The lysine-rich C-terminal repeats of the centromere-binding factor 5 (Cbf5) of Kluyveromyces lactis are not essential for function (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 14 (1998), S. 37-48 
    Details
    ISSN: 0749-503X
    Keywords: Kluyveromyces lactis ; CBF5 ; centromere ; nucleolus ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The gene coding for the centromere-binding factor 5 (CBF5) of Kluyveromyces lactis has been isolated by hybridization of a Saccharomyces cerevisiae CBF5 DNA probe to a K. lactis library. The amino acid sequence of KlCbf5 is highly homologous, 88% identity, to ScCbf5, but also to the rat protein Nap57 (64% identity). The main difference between both yeast proteins and the rat protein is the presence of a lysine-rich domain with KKE/D repeats in the C-terminal part of the protein. These repeats are thought to be involved in binding of the protein to microtubules. Deletion of the KKE/D domain in KlCbf5 however, has no discernible effect on growth on rich medium, sensitivity to the microtubule-destabilizing drug benomyl or segregation of a reporter plasmid. On the other hand, insertion of two leucine residues adjacent to the KKE domain increases the loss rate of a reporter plasmid. In both yeasts complementation of a lethal CBF5 disruption with the heterologous gene results in a slight increase in benomyl sensitivity. A possible role of CBF5 in chromosome segregation will be discussed. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Über eine Reihe saurer Verbindungen HXVP2O8 und H2XIVP2O8 mit Schichtstruktur, XV = As und Sb; XIV = Si, Ge, Sn, Pb, Ti und Zr (1966)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 346 (1966), S. 92-112 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: The new As(V)—P(V) compound HAsP2O8 is prepared and shown to have a layer structure built up by AsP2O8 layers and inter-layer protons which are exchangeable by other cations.The compound HSbP2O8 is isomorphous with HAsP2O8; a series of H2XIVP2O8 compounds with XIV = Si, Ge, Sn, Pb, Ti and Zr is prepared having analogous layer structures and also ion exchanger properties.
    Notes: Es wird die Verbindung HAsP2O8 hergestellt und aus ihren Eigenschaften geschlossen, daß es sich um eine Verbindung mit Schichtstruktur handelt, in der die Protonen zwischen AsP2O8-Schichten liegen und durch Kationen ersetzbar sind.Im zweiten Teil wird gezeigt, daß die Verbindung HSbP2O8 mit HAsP2O8 isomorph ist und daß sich außerdem eine ganze Reihe von Verbindungen H2XIVP2O8 mit XIV = Si, Ge, Sn, Pb, Ti und Zr herstellen läßt, die analog gebaute Schichtstrukturen bilden und ebenfalls Ionenaustauschereigenschaften haben.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19663460110
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  • 8
    Electronic Resource
    Electronic Resource
    Über K2Si(S2O7)3, eine weitere SiO2—SO3-Verbindung (1969)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 365 (1969), S. 180-184 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: (K2SiO3)x with highpolymeric chain anions reacts with SO3 to from the compound K2SiO3 · 6 SO3 which contains the SO3 as S2O72· groups and the Si as isolated SiOn polyhedra. Properties of the compound, thus to be formulated as K2Si(S2O7)3, are described and possible structures discussed.
    Notes: (K2SiO3)x mit hochpolymerem Kettenanion reagiert mit SO3 zur Verbindung K2SiO3 · 6 SO3, in der das SO3 in Form von S2O7-Gruppen gebunden ist und das Si in isolierten Silicium-Sauerstoff-Polyedern vorliegt. Eigenschaften der damit als K2Si(S2O7)3 zu formulierenden Verbindung werden beschrieben und Strukturmöglichkeiten diskutiert.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19693650312
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