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  • 2000-2004  (1)
  • 1995-1999  (2)
  • Genetic resources  (2)
  • Alliaceae  (1)
  • Molecular markers
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  • 1
    Electronic Resource
    Electronic Resource
    Are mapped markers more useful for assessing genetic diversity? (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 607-613 
    Details
    ISSN: 1432-2242
    Keywords: Key words AFLP ; Biodiversity ; Genetic maps ; Genetic resources ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genetic diversity within populations of organisms and species is commonly measured using molecular-marker data. It has been claimed that more reliable diversity measurements can be obtained using selected genetically mapped markers to ensure that all regions of the genome are represented in the data sets employed. However, this has not been tested. In the present study, using rice (Oryza sativa L.) as a model species, we have shown that the use of unmapped AFLP markers reveals a pattern of diversity that is very similar to that obtained using a range of other marker types and which reflects the known crossability groups within this species. In contrast, we show that use of mapped-marker data can, in some cases, result in highly misleading patterns of diversity; the results obtained are critically related to the choice ofparents used in the cross from which the mapping population was produced. For diversity analyses, we propose that it is appropriate to use unmapped markers provided that the marker-type has been shown to have a wide distribution over the genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050080
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The identification of duplicate accessions within a rice germplasm collection using RAPD analysis (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 1049-1055 
    Details
    ISSN: 1432-2242
    Keywords: Oryza sativa ; Rice ; Genetic resources ; RAPD ; Molecular markers ; Cluster analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A set of accessions of Oryza sativa from the International Rice Research Institute (Philippines) that included known and suspected duplicates as well as closely related germplasm has been subjected to RAPD analysis. The number of primers, the number of polymorphic bands and the total number of bands were determined that will allow the accurate discrimination of these categories of accessions, including the identification of true and suspected duplicates. Two procedures have been described that could be employed on a more general basis for identifying duplicates in genetic resources collections, and further discussion on the values of such activities is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222920
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Distribution of a 375 bp repeat sequence inAllium (Alliaceae) as revealed by FISH (1999)
    Springer
    Plant systematics and evolution 217 (1999), S. 31-42 
    Details
    ISSN: 1615-6110
    Keywords: Alliaceae ; Allium ; Fluorescent in-situ hybridisation ; cytotaxonomy ; telomeric repeat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Information about evolutionary relationships between species of the genusAllium is desirable in order to facilitate breeding programmes. One approach is to study the distribution of repetitive DNA sequences among species thought on taxonomic grounds, to be closely related. We have used fluorescent in-situ hybridisation (FISH) to examine seven species within sect.Cepa of the genus (A. altaicum, A. cepa, A. fistulosum, A. galanthum, A. pskemense, A. oschaninii andA. vavilovii), one species from sect.Rhizirideum (A. roylei), two species from sect.Allium (A. sativum andA. porrum) and one species from sect.Schoenoprasum (A. schoenoprasum). Each species was probed using a 375 bp repeat sequence isolated fromA. cepa (Barnes & al. 1985), which was generated and labelled by polymerase chain reaction (PCR). No signals were detected in anyAllium species not belonging to sect.Cepa with the exception ofA. roylei, whose designation in sect.Rhizirideum is now questioned. Within sect.Cepa the probe was found to hybridize to the ‘terminal’ regions of the chromosome arms of all the species examined. In addition a number of interstitial bands were detected. Use of FISH reveals a more detailed map of the location of the repeat sequences than has previously been obtained by C-banding and other staining procedures. The distribution of the terminal and interstitial sites when compared, allow us to identify three species groups namely,A. altaicum andA. fistulosum; A. cepa, A. roylei, A. oschaninii andA. vavilovii; andA. galanthum andA. pskemense.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984920
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    Paper (German National Licenses)
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