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  • 2000-2004  (1)
  • 1995-1999  (1)
  • Key wordsBordetella spp.  (1)
  • PACS. 74.72.-h High Tc compounds – 74.25.Fy Transport properties (electric and thermal conductivity, thermoelectric effects, etc.)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 262 (1999), S. 189-198 
    ISSN: 1617-4623
    Keywords: Key wordsBordetella spp. ; Evolution ; Lipopolysaccharide biosynthesis ; Pulsed-field gel electrophoresis ; Representational difference analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bordetella pertussis and B. bronchiseptica are genetically very closely related but differ significantly in their virulence properties. Using Representational Difference Analysis (RDA), 11 DNA fragments specific for B. pertussis Tohama I or B. bronchiseptica BB7865 were identified. All B. bronchiseptica BB7865-derived fragments also hybridized with chromosomal DNA from B. parapertussis but not from the B. pertussis strains Tohama I and W28, underlining the close phylogenetic relationship between B. bronchiseptica and B. parapertussis. The B. pertussis type strain BP18323 is a special case, as it contains DNA sequences characteristic for both B. pertussis and B. bronchiseptica. As demonstrated by pulsed-field gel electrophoresis, several of the BB7865-derived fragments are present on a single 30-kb XbaI fragment. Based on the sequences of putative coding regions, four of these fragments may code for proteins involved in carbohydrate metabolism or transport. In agreement with this notion, a mutant for one of these loci synthesizes a significantly altered lipopolysaccharide that lacks the O-specific side chains. The analysis of the corresponding genomic region in various Bordetella species showed that this locus is present in B. bronchiseptica and B. parapertussis but not in B. pertussis. This confirms that the RDA approach has identified a novel strain-specific LPS biosynthesis locus which accounts for the differences between the LPS structures elaborated by different Bordetella species.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1434-6036
    Keywords: PACS. 74.72.-h High Tc compounds – 74.25.Fy Transport properties (electric and thermal conductivity, thermoelectric effects, etc.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: Measurements of the thermal conductivity (kxx) and the thermal Hall effect (kxy) in high magnetic fields in Y- and Bi-based high-T c superconductors are presented. We describe the experimental technique and test measurements on a simple metal (niobium). In the high-T c superconductors kxx and kxy increase below T c and show a maximum in their temperature dependence. kxx has contributions from phonons and quasiparticle (QP) excitations, whereas kxy is purely electronic. The strong increase of kxy below T c gives direct evidence for a strong enhancement of the QP contribution to the heat current and thus for a strong increase of the QP mean free path. Using kxy and the magnetic field dependence of kxx we separate the electronic thermal conductivity ( k xx el ) of the CuO 2 -planes from the phononic thermal conductivity ( k xx ph ). In YBa2Cu3O 7 - δ k xx el shows a pronounced maximum in the superconducting state. This maximum is much weaker in Bi2Sr2CaCu2O 8 + δ , due to stronger impurity scattering. The maximum of k xx el is strongly suppressed by a magnetic field, which we attribute to the scattering of QPs on vortices. An additional magnetic field independent contribution to the maximum of kxx occurs in YBa2Cu3O 7 - δ , reminiscent of the contribution of the CuO-chains, as determined from the anisotropy in untwined single crystals. Our data analysis reveals that below T c as in the normal state a transport (τ) and a Hall ( ) relaxation time must be distinguished: The inelastic (i.e. temperature dependent) contribution to τ is strongly enhanced in the superconducting state, whereas displays the same temperature dependence as above T c . We determine also the electronic thermal conductivity in the normal state from kxy and the electrical Hall angle. It shows an unusual linear increase with temperature.
    Type of Medium: Electronic Resource
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