ISSN:
0749-1581
Keywords:
19F NMR
;
Fluorinated calcium chelators
;
Enhanced relaxation characteristics
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The fluorinated calcium ion chelator 5F BAPTA [1,2-bis(2-amino-5-fluorophenoxy)ethane-N, N, N′, N′-tetra-acctic acid] was used for the determination of cytosolic Ca2+ ion levels in cells and tissues by 19F NMR. The possibility of enhancing the sensitivity of these measurements by modifying the relaxation behavior of the chelator was evaluated. These strategies involved the design of a chelator with reduced T1 value viz. 5F RBAPTA {1,2-bis [2-amino-4-(1-carboxy-2-methyl-2-propyl)-5-fluorophenoxy]ethane-N, T, N′, N-tetraacetic acid}, and the evaluation of chelator with slower exchange characteristics, viz 5F BenzEGTA [1-(2-amino-5-fluorophenoxy)-2-(2-aminoethoxy)ethane-N, N, N′, N′-tetraacetic acid]. Both analogs exhibit chemical shift differences on calcium complexation which are similar to those of the parent compound, 5F BAPTA. In the case of BenzEGTA, a considerably greater pH dependence of the apparent calcium, dissociation constant, KD, also results from the presence of a tertiary amine with a pK value above the physioligical range. Additionally, the synthetic approach used for RBAPTA can lead to wide variety of derivatives with variable chemical, biochemical and NMR properties.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260300807
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