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  • 2000-2004  (1)
  • 1990-1994  (2)
  • 25kDa amelogenin  (1)
  • Continuous ventricular function monitor  (1)
  • Keywords: infrared radiation; pasteurization; antibiotic; Escherichia coli; injured cell; lethal temperature  (1)
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  • 2000-2004  (1)
  • 1990-1994  (2)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 24 (2000), S. 19-24 
    ISSN: 1476-5535
    Keywords: Keywords: infrared radiation; pasteurization; antibiotic; Escherichia coli; injured cell; lethal temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Escherichia coli in phosphate-buffered saline irradiated with far-infrared (FIR) energy was injured and killed even under the condition where the bulk temperature of the suspension was maintained below the lethal temperature. Using four kinds of antibiotics (penicillin G, chloramphenicol, nalidixic acid and rifampicin), we investigated the FIR irradiation-induced damage to E. coli on the basis of the sensitivity changes to the antibiotics. FIR irradiation increased the organism’s sensitivity to rifampicin both below and above the lethal temperature. The increase in sensitivity to chloramphenicol was observed only when FIR irradiation occurred above the lethal temperature. These results suggest that the mechanism of FIR irradiation-induced death in E. coli differs according to whether the radiation exposure occurs above or below the lethal temperature. Journal of Industrial Microbiology & Biotechnology (2000) 24, 19–24.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 51 (1992), S. 213-217 
    ISSN: 1432-0827
    Keywords: Porcine secretory enamel ; Degradation of amelogenin ; Proteinases ; 25kDa amelogenin ; Enzymography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary In the outermost layer of porcine-developing enamel adjacent to the ameloblasts in the secretory stage, the activities of two proteinases having molecular masses of 76 and 78kDa were detected by enzymography using gelatin as a substrate. On the other hand, high activities of known 30 and 34kDa proteinases were localized in the inner layer of the enamel. The 76kDa proteinase cleaved the carboxylterminal peptide of porcine 25kDa amelogenin to convert it to 20kDa amelogenin. The 78kDa proteinase also acted on the 25kDa amelogenin similarly, but its activity was weak. The results indicate that the 25kDa amelogenin synthesized and secreted by ameloblasts is converted to 20kDa amelogenin by the action of proteinase localized in the outermost layer of the secretory enamel, and then further degraded by the proteinases in the inner layer of the enamel associated with the increase of mineralization.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1619-7089
    Keywords: Syndrome X ; Continuous ventricular function monitor ; Supine exercise ; VEST
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Left ventricular function during exercise and recovery was investigated in patients with angina pectoris, ST segment depression during exercise and angiographically normal coronary arteries (syndrome X) using a continuous left ventricular function monitor with cadmium telluride detector (CdTe-VEST). Fourteen patients with syndrome X and 14 patients with atypical chest pain without ST segment depression during exercise and normal coronary arteries (control group) performed supine ergometric exercise after administration of 740–925 MBq of technetium-99m labelled red blood cells, and left ventricular function was monitored every 20 s using CdTe-VEST. Left ventricular ejection fraction (EF) response was impaired (≤55% increase from rest to peak exercise) in 11 or 14 patients with syndrome X but in none of the control patients. Resting EF was similar in the two groups (62.1%±6.7% in patients with syndrome X, 61.9%±6.2% in controls); however, EF increase from rest to peak exercise was lower in syndrome X (−3.1±9.5% vs 14.7%±7.4%, P 〈0.001). After cessation of exercise, all patients showed rapid EF increase over baseline and this EF overshoot was lower (19.3%±8.3% vs 26.4%±7.3%, P 〈0.001) with the time to EF overshoot longer (114±43 s vs 74±43 s, P〈0.05) in patients with syndrome X. Thus, in patients with syndrome X, left ventricular dysfunction was frequently observed during exercise in spite of normal epicardial coronary arteries.
    Type of Medium: Electronic Resource
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