ISSN:
1573-5001
Keywords:
3D NMR
;
J coupling constants
;
Isotopic labeling
;
Protein structure
;
ϕ-angle
;
Soft HNCA-COSY
;
Soft HNCA-E.COSY
;
Gradient-enhanced spectroscopy
;
Sensitivity-enhanced spectroscopy
;
Ribonuclease T1
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the 3J(HN,Hα) and 3J(HN,C′) coupling constants in uniformly 13C- and 15N-labeled proteins. They employ gradient coherence selection in combination with the sensitivity enhancement method in HSQC-type spectra (Cavanagh et al., 1991; Palmer et al., 1991). In most cases, the two measured coupling constants unambiguously define the ϕ-angle for protein structure determination. The method is applied to uniformly 13C, 15N-labeled ribonuclease T1.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00175250
Permalink