Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 2000-2004  (2)
  • 1985-1989
  • Embryonic stem cell Expressed sequence tag Neural differentiation Gene discovery  (1)
  • Raman spectrum  (1)
Source
Material
Years
  • 2000-2004  (2)
  • 1985-1989
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    ES cell neural differentiation reveals a substantial number of novel ESTs (2000)
    Springer
    Functional & integrative genomics 1 (2000), S. 127-139 
    Details
    ISSN: 1438-7948
    Keywords: Embryonic stem cell Expressed sequence tag Neural differentiation Gene discovery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. We have used a method for synchronously differentiating murine embryonic stem (ES) cells into functional neurons and glia in culture. Using subtractive hybridization we isolated approximately 1200 cDNA clones from ES cell cultures at the neural precursor stage of neural differentiation. Pilot studies indicated that this library is a good source of novel neuro-embryonic cDNA clones. We therefore screened the entire library by single-pass sequencing. Characterization of 604 non-redundant cDNA clones by BLAST revealed 96 novel expressed sequence tags (ESTs) and an additional 197 matching uncharacterized ESTs or genomic clones derived from genome sequencing projects. With the exception of a handful of genes, whose functions are still unclear, most of the 311 known genes identified in this screen are expressed in embryonic development and/or the nervous system. At least 80 of these genes are implicated in disorders of differentiation, neural development and/or neural function. This study provides an initial snapshot of gene expression during early neural differentiation of ES cell cultures. Given the recent identification of human ES cells, further characterization of these novel and uncharacterized ESTs has the potential to identify genes that may be important in nervous system development, physiology and disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101420000014
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Free Hydrogen Bonds in Alcohol Solutions with Inert Solvents (2000)
    Springer
    Journal of solution chemistry 29 (2000), S. 1007-1016 
    Details
    ISSN: 1572-8927
    Keywords: Raman spectrum ; free hydrogen bond ; alcohol ; inert solvent ; solution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Raman spectra were obtained for alcohol solutions with inert solvents such asCCl4, CHCl3, etc. The frequency of the OH stretching vibrations[νOH(free) band]due to free hydrogen bonds varies with change in either alcohol or inert solvent.It is shown that the frequency of the νOH(free) band is sensitive to the shape ofthe head group of an inert solvent. The main cause for the frequency change ofthe νOH(free) band with change in alcohol is ascribed to the change of effectivemass of the vibrating unit (RO-H, R = alkyl group).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005142919076
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...