ISSN:
1432-0428
Keywords:
Islet cell antibodies
;
indirect immunofluorescence
;
insulin-dependent diabetes
;
assay reproducibility
;
assay precision
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The sensitivity and specificity of the assay for islet cell cytoplasmic antibodies in human serum were examined using cryostat sections from fresh frozen pancreas. The specificity of the assay was close to 100% while the sensitivity was 40%–98% depending on the pancreas used. Inter-observer variation was 12–27%. End-point titres of islet cell antibodies varied with the sensitivity of each pancreas. End-point titration of the antibodies in two different laboratories using the same pancreas was significantly correlated (Spearman test p 〈 0.001). We conclude that a reliable determination of islet cell antibody titres in human serum requires careful characterization of the sensitivity and specificity of each pancreas used as a source of frozen sections, in the indirect immunofluorescence assay.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00250895
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