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Effect of pH and Zn2+ on subcultured muscle cells from Macrobrachium nipponense (2000)

Wang, Wei-Na ; Liang, Hong ; Wang, An-Li ; [et al.]

Springer

Methods in cell science 22 (2000), S. 277-284

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ISSN: 1573-0603

Keywords: Macrobrachium nipponense ; Cell subculture ; pH ; Zn2+

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A cell culture system was devised for muscle cell of Macrobrachium nipponense in the study. The juvenile and adult shrimps were held in laboratory aquaria with penicillin 1000 IU/ml and streptomycin 1000 µg/ml for 12–24 hours. Cell cultures were established in medium 199 supplemented with 20% fetal bovine serum, 1 g/L glucose, 5.2 g/L NaCl, 1.43 g/L CaCl2, 0.05 g/L MgCl2, 100 IU/mL penicillin and 100 µg/ml streptomycin. Fibroblast-like cells were passaged up to three times and survived for 54 days. The results showed the optimum for subculture in vitro was in medium 199 with pH 7.6. Moreover, basal medium supplemented with Zn2+ 60 µg/L could enhance the growth of the muscle cells. It was found that better results for cell culture would be obtained more easily with juvenile shrimps caught in spring than adults in summer or autumn; and shrimps caught within 12 hours after ecdysis could grow much better than the intermoult shrimps.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1017962429862

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Effectiveness factor calculations for immobilized enzyme catalysts (1973)

Fink, David J. ; Na, Tsungyen ; Schultz, Jerome S.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 15 (1973), S. 879-888

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: The steady state, nonlinear diffusion equations which describe reactions in constrained enzyme solutions are of great interest in many biological and engineering applications. As in other types of nonlinear differential equations, exact analytical solutions do not exist except in some simplified cases. In this paper, a general procedure is presented for solving numerically for the substrate concentration profile and effectiveness factor utilizing the transformation method suggested by Na and Na. Design correlations for enzyme solutions constrained within spherical membranes are included. The use of a unique definition of the Thiele Modulus in these charts permits the clear illustration of the effects of substrate concentration and external mass transfer resistances on the overall effectiveness factor for the catalyst particle.

Additional Material: 3 Ill.