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Rational Design and Synthesis of Ribozymes (2000)

Welz, R. ; Schmidt, C. ; Müller, S.

Springer

Molecular biology 34 (2000), S. 934-939

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ISSN: 1608-3245

Keywords: catalytic RNA ; ribozyme design ; RNA synthesis ; fluorescent oligonucleotides ; reaction kinetics

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract We review our procedure for the rational design and the synthesis of oligoribonucleotides with particular reference to our recent studies of the development of a hairpin-derived twin ribozyme. Our work focuses on the design of ribozymes that are derived from naturally occurring structures but posses extended catalytic activities. Currently we are studying ribozymes that are capable of site-specific RNA double cleavage. We have designed these “twin ribozymes” by combining of two catalytic motifs in one molecule. In addition to kinetic analysis of the cleavage reaction, we are interested in the general phenomena of RNA folding and the characterization of RNA structure–function relationships. The developed twin ribozymes are particularly well suited for this purpose. They have to fold into a complex structure to achieve catalytic activity. Since there is a direct correlation between conformation and activity, the process of folding can be followed by monitoring the cleavage reaction. The twin ribozymes were synthesized by a combination of phosphoramidite chemistry and in vitro transcription techniques. We report the synthesis of a powerful reagent to replace tetrazole for activation of RNA phosphoramidites in automated solid-phase synthesis, allowing for coupling yields of 〉99%. Furthermore, we introduce a fast quantitative assay of ribozyme activity which is based on fluorescently labeled oligoribonucleotide substrates and DNA sequencer technology.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1026644313406

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Morphologische Besonderheiten polymerer Mehrkomponentensysteme auf der Basis von Dithiolen und Diisocyanaten (1979)

Lebedev, E. V. ; Lipatov, Ju. S. ; Müller, S. ; [et al.]

Weinheim : Wiley-Blackwell

Acta Polymerica 30 (1979), S. 496-502

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ISSN: 0323-7648

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Physics

Description / Table of Contents: Polyurethanes obtained by reacting mercapto-terminated oligobutadienes, decanedithiol and diisocyanate were investigated by electron microscopy and x-ray scattering. The dependence of the morphological structure on the content of short-chain dithiol is discussed. Heterogeneities in micro- and macroregions were observed, which are caused by crystalline regions and phase separation in chemically different blocks.

Notes: Polyurethane auf der Basis von mercapto-terminierten Oligobutadienen, Decandithiol und Diisocyanat werden mittels Elektronenmikroskopie und Röntgenstreuung untersucht. Ihre morphologischen Besonderheiten werden in Abhängigkeit vom Gehalt an Kurzkettendithiol diskutiert. Es treten Heterogenitäten im Mikro- und Makrobereich auf, die durch kristalline Bereiche und Phasentrennung in chemisch unterschiedliche Blöcke bedingt sind.

Additional Material: 7 Ill.