ISSN:
1608-3245
Keywords:
EcoRII restriction endonuclease
;
EcoRII methylase
;
histidine derivatives
;
DNA–RNA hybrids
;
nucleic acid–protein interaction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract To obtain recombinant restriction endonuclease (R) and methylase (M) of the EcoRII restriction–modification system, bacterial strains overproducing their functional hexahistidine derivatives were constructed. Active full-length R · EcoRII was produced only in cells that also expressed M · EcoRII from a multicopy plasmid. Recombinant R · EcoRII bound with hybrid DNA · RNA duplexes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1026688028427
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