ISSN:
1432-203X
Keywords:
Key words Aspartate aminotransferase
;
Phosphoenolpyruvate carboxylase
;
Isoenzyme
;
Transgenic tobacco
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Expression of Panicum miliaceum L. (proso millet) mitochondrial and cytosolic aspartate aminotransferase (mAspAT and cAspAT, respectively) genes in transgenic tobacco plants (Nicotiana tabacum) and their influences on protein synthesis were examined. The mAspAT- or cAspAT-transformed plants had about threefold or 3.5-fold higher AspAT activity in the leaf than non-transformed plants, respectively. Interestingly, the leaves of both transformed plants had increased levels of phosphoenolpyruvate carboxylase (PEPC) and transformed plants with cAspAT also had increased levels of mAspAT in the leaf. These results suggest that the increased expression of Panicum cAspAT in transgenic tobacco enhances the expression of its endogenous mAspAT and PEPC, and the increased expression of Panicum mAspAT enhances the expression of its endogenous PEPC.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050779
Permalink
