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  • 2000-2004  (1)
  • 1975-1979  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Inhibition of nuclear factor kappa B activation and inducible nitric oxide synthase transcription by prolonged exposure to high glucose in the human keratinocyte cell line HaCaT (2004)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 150 (2004), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  In human skin, inducible nitric oxide synthase (iNOS) appears to be a key enzyme during wound healing and has roles in protection from infection. We speculated that diabetic skin complications such as delayed wound healing and skin infection were due to iNOS activity altered by high glucose in skin keratinocytes.Objectives  The purpose of this study was to see how high levels of glucose affect iNOS activity in the human keratinocyte cell line (HaCaT).Methods  HaCaT cells were exposed to high glucose for 1 day or 10 days. We measured nitric oxide (NO) end product nitrite in the culture medium using the Griess reagent, and intracellular tetrahydrobiopterin (BH4, a cofactor of NOS) content by using high-performance liquid chromatography, analysed the expression level of iNOS mRNA by the reverse transcriptase-polymerase chain reaction method and evaluated the DNA binding activity of nuclear factor kappa B (NF-κB) by enzyme-immunoassay.Results  Short-term exposure (1 day) to a high level of glucose increased BH4 and iNOS activity at the post-translational level. However, long-term exposure (10 days) to high glucose downregulates NF-κB binding activity and inhibits iNOS transcription and its activity.Conclusions  Pretreatment with high glucose for 10 days down-regulated NF-κB activity and inhibited iNOS transcription and NO production, implying the involvement of a deficiency in NO synthesis in both skin infection and impaired wound healing in diabetic patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0007-0963.2004.05867.x
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  • 2
    Electronic Resource
    Electronic Resource
    A field survey on the health status of workers in dye-producing factories (1977)
    Springer
    International archives of occupational and environmental health 39 (1977), S. 219-235 
    Details
    ISSN: 1432-1246
    Keywords: Anaemia ; Aromatic nitro-amino compounds ; Diazo-positive urinary metabolites ; Heinz bodies ; Hepatotoxicity ; Methemoglobinaemia ; Methemoglobin reductases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Health status of the workers in dye-producing plants were examined repeatedly in winter and in summer. The items studied cover 1) diazo-positive metabolites in urine 2) methemoglobinaemia, Heinz bodies, methemoglobin reductases and anaemia, and 3) liver function. The urinary diazo-positive metabolite level in the exposed was higher than that in the non-exposed and marked a conspicuous increase in summer as compared in winter, indicating significant intake of the aromatic nitro-amino compounds, while no significant methemoglobinaemia, Heinz bodies, anaemia nor disturbed liver function was observed in any group of the examinees. The results were discussed in connection with the dose-effect relationship, and compared with the experiences in the past.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409368
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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