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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and identification of the major concanavalin A binding glycoproteins from murine lymphocytes (1976)
    s.l. : American Chemical Society
    Biochemistry 15 (1976), S. 2698-2705 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00657a033
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  • 2
    Electronic Resource
    Electronic Resource
    Rice-soil interactions in Vietnamese acid sulphate soils: impacts of submergence depth on soil solution chemistry and yields (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 17 (2001), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. The aim of this study was to investigate the effects of water submergence depth on radial oxygen loss (ROL), soil solution chemistry and rice growth performance in acid sulphate soils in southern Vietnam. ROL was measured in a solution culture. In a separate pot experiment the impact of water submergence depth on rice growth and soil solution chemistry was studied. Three submergence depths were used in the two experiments (5, 10 and 15 cm). ROL declined with submergence depth and was significantly greater in young roots (with no root hairs) than in older roots. In the pot experiment rice growth and soil solution chemistry were clearly affected by the submergence depth. During the first crop at 5 cm submergence, there was a significantly higher yield and a higher oxidation state (pe+pH) compared to 10 or 15 cm submergence. The Fe concentration was significantly greater at the 5 cm depth compared to the 10 or 15 cm depth. SO42– reduction was delayed at the 5 cm depth. Rice yield was c. 25% less at the 15 cm than at the 5 cm depth. During a second crop, there was a substantial SO42- reduction and H2S formation and almost no significant effects of submergence depth on either soil solution chemistry or crop yield. In a field experiment with a dry-season rice crop, yield and Fe, Al and SO42– concentrations were higher at a shallow submergence depth than at greater depths in the same field, showing similar depth trends to those found during the first crop in the pot experiment. Farmers should be advised to use a shallow submergence depth and, if possible, avoid deep-rooted rice varieties. A conceptual model is suggested, which summarizes the relationships between ROL and soil solution chemistry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2001.tb00011.x
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  • 3
    Electronic Resource
    Electronic Resource
    Concanavalin-A-binding Proteins on the Surface of Human Malignant and Normal Lymphocytes (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 9 (1979), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The concanavalin-A-binding cell surface glycoproteins from normal and certain leukaemic human lymphocytes were radiolabelled and then solubilized with detergent, isolated by affinity chromatography on Con A insolubilized on agarose beads, and subsequently analysed by SDS-polyacrylamide gel electrophoresis. Leukaemic T cells from patients with Sezary syndrome were found to express major concanavalin-A-binding glycoproteins on their outer surface similar to those of normal T lymphocytes. Leukaemic B cells from patients with chronic lymphocytic leukaemia expressed C on-A-binding proteins similar to those of B-cell lines. HLA antigens were predominant among the major Con-A-binding proteins on the surface of the normal and the malignant T cells studied. Human la-like antigens, HLA antigens, and the cell surface immunoglobulins IgD and IgM represented the major Con-A-binding proteins on the B cells studied. β2-microglobulin was found associated with HLA antigens on both leukaemic and non-leukaemic T and B cells. The presence of additional Con-A-binding proteins expressed on the surface of the different cell types studied is discussed along with some physical characteristics of the human la-like antigens isolated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1979.tb03275.x
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation and Partial Characterization of Rat Lymphoid Cell Surface Histocompatibility Antigens and Immunoglobulins (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 7 (1978), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Cell surface molecules of rat normal lymphoid cells were selectively labelled by lactoperoxidase catalysed iodination or by a galactose oxidase tritiated sodium borohydride technique, subsequently detergent solubilized, isolated by indirect immunoprecipitation and analysed on SDS-polyacrylamide gel electrophoresis. Four polypeptide chains were isolated by using the alloantiserum DA anti-Lewis. The molecular weights of the antigens were calculated as 41,000, 33,000, 27,000 and 12,000. Based on functional in vitro characteristics of the antiserum used and on the physiochemical properties as well as genetics of inheritance and tissue distribution, the polypeptide chains were identified as being subunits of Ag-B and Ia antigens. Two types of immunoglobulin heavy chains exhibiting the molecular weights 70,000 and 64,000 were isolated from unfractionated normal spleen cells by use of a polyvalent rabbit anti-rat immunoglobulin serum and tentatively identified as μ and δ chain. Using the same anti-immunoglobulin serum, no molecules could be precipitated from the lysates of Lewis thymocytes or peripheral T cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1978.tb00458.x
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  • 5
    Electronic Resource
    Electronic Resource
    Periwound dopaminergic sprouting is dependent on numbers of wound macrophages (2002)
    Oxford, UK : Blackwell Science, Ltd
    European journal of neuroscience 15 (2002), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Injury to many regions of the central nervous system, including the striatum, results in a periwound or ‘abortive’ sprouting response. In order to directly evaluate whether macrophages play an important role in stimulating periwound sprouting, osteopetrotic (op/op) mice, which when young are deficient in a variety of macrophage subtypes, were given striatal wounds and the degree of dopaminergic sprouting subsequently assessed. Two weeks postinjury, significantly fewer wound macrophages were present in the striata of op/op mice compared with controls (144 ± 30.1 in op/op mice vs. 416.6 ± 82.3 in controls, P 〈 0.005, analysis performed on a section transecting the middle of the wound). Dopamine transporter immunohistochemistry revealed a marked decrease in the intensity of periwound sprouting in the op/op group of animals. Quantification of this effect using [H3]-mazindol autoradiography confirmed that periwound sprouting was reduced significantly in the op/op mice compared with controls (71.4 ± 21.7 fmol/mg protein in op/op mice vs. 210.7 ± 27.1 fmol/mg protein in controls, P 〈 0.0005). In the two groups of animals the magnitude of the sprouting response in individuals was closely correlated with the number of wound macrophages (R = 0.83, R2 = 0.69). Our findings provide strong support for the crucial involvement of macrophages in inducing dopaminergic sprouting after striatal injury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-9568.2002.01914.x
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  • 6
    Electronic Resource
    Electronic Resource
    Stronger association with HLA-Cw6 than with corneodesmosin (S-gene) polymorphisms in Swedish psoriasis patients (2000)
    Springer
    Archives of dermatological research 292 (2000), S. 525-530 
    Details
    ISSN: 1432-069X
    Keywords: Keywords Cw6 ; HLA antigens ; Linkage ; disequilibrium ; Psoriasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Psoriasis vulgaris is strongly associated with certain human leukocyte antigens, especially in early onset. The purpose of this study was to study the HLA-Cw6 allele and its contribution to disease susceptibility in a set of 104 families with at least two affected siblings. A sequencing method was utilized to examine the two exons that build up the antigen binding site of the C locus receptor. DNA from patients homozygous for Cw6 based on haplotype information were sequenced. The results confirmed the identity of the Cw6 allele in affected individuals with the consensus sequence for Cw*0602. We screened the set of families for psoriasis patients homozygous for Cw6 and found 11 individuals with a mean age at onset of 16.1 years. The corresponding figure for the Cw6 heterozygotes was 18.45 years and for the Cw6-negatives 22.36 years. This is indicative of a gene dose effect. We performed a transmission disequilibrium test (TDT) on the Cw6 allele per se, used as a biallelic marker. The analysis resulted in a P-value of 5.3 × 10–17 (t167/nt45). This greatly exceeds our previous results of a TDT in the region, including microsatellite markers and single nucleotide polymorphisms (SNPs) in the coding part of the S gene (corneodesmosin), which is a suggested candidate gene in the region. The maximum nonparametric linkage (NPL) value was also reached using HLA-C as a marker. We conclude that Cw6 is the allele which shows the highest degree of association with psoriasis in our set of families and we propose that it directly influences the age at onset of the disease rather than increasing the genetic load in accordance with a polygenic theory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004030000175
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  • 7
    Electronic Resource
    Electronic Resource
    Calcium content of liming material and its effect on sulphur release in a coniferous forest soil (2000)
    Springer
    Biogeochemistry 50 (2000), S. 1-20 
    Details
    ISSN: 1573-515X
    Keywords: calcium content ; DOC ; liming material ; pH ; podsol ; sulphur mineralization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Soil columns with O + A (Experiment I) or Ohorizons (Experiment II) from a Haplic Podsol wereincubated at 15 °C for 368 and 29 + 106 days,respectively. Three types of liming material differingin Ca2+ content, i.e. calcium carbonate(CaCO3), dolomite (CaMg(CO3)2) andmagnesium carbonate (MgCO3), were mixed into theO horizons in equimolar amounts corresponding to 6000kg of CaCO3 per ha. In the limed treatments ofExperiment I, the leaching of dissolved organic carbon(DOC) and the net sulphur mineralization (estimated asaccumulated SO 4 2− leaching corrected forchanges in the soil pools of adsorbed and waterextractable SO 4 2− ) increased with decreasingCa2+ content of the lime and increasing degree oflime dissolution. In relation to the controltreatment, only the MgCO3 treatment resulted ina significantly higher net sulphur mineralization. InExperiment I the net sulphur mineralization was 4.06,1.68, 0.57, and 2.14 mg S in the MgCO3,CaMg(CO3)2, CaCO3 and control treatment,respectively. The accumulated SO 4 2− leachingin Experiment II during the first 29 days was 1.70,0.74 and 0.48 mg S in the MgCO3,CaMg(CO3)2 and control treatment,respectively. In the two experiments there wereconsistently significant positive correlations betweenleached amounts of SO 4 2− and DOC. It wasconcluded that net sulphur mineralization was stronglyconnected to the solubilization of the organic matter(DOC formation) and that pH and/or Ca2+ ionsaffected the net sulphur mineralization through theireffects on organic matter solubility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006355532514
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  • 8
    Electronic Resource
    Electronic Resource
    The effects of vitamin E on serum enzyme levels following heavy exercise (1979)
    Springer
    European journal of applied physiology 40 (1979), S. 283-289 
    Details
    ISSN: 1439-6327
    Keywords: Vitamin E ; d-α-Tocopherol ; Serum enzymes ; Creatine kinase ; Exercise
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of vitamin E (d-α-tocopherol) on serum enzyme levels after heavy exercise with trained and untrained muscles were studied in 26 subjects, aged 19–24, using a double blind experimental design. After a 6 week medication period (300 mg daily), the serum concentration of d-α-tocopherol had increased from 12.7–19.6 Μg/ml in the vitamin E group, while in the placebo group the value was unchanged. The subjects were then exposed to muscular work with either trained or untrained muscles. Serum concentrations of CK, ASAT and LD were measured prior to exercise and at different time intervals afterwards. In subjects exposed to exercise with trained muscles there was an insignificant elevation in serum enzymes and no indication of any difference between the two groups. Muscular work involving mainly untrained muscles caused a highly significant increase in serum CK activity. A significant elevation was also observed for ASAT and LD. No difference between the vitamin E and the placebo group was found. Isoenzyme studies showed that the rise in CK was due to an increase in the CK-MM fraction, indicating that striated muscle was the major site of enzyme release. It is concluded that vitamin E has no influence on the increase in serum enzyme levels following exercise.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421520
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  • 9
    Electronic Resource
    Electronic Resource
    Are nest characters of importance when choosing a male in the fifteen-spined stickleback (Spinachia spinachia)? (2000)
    Springer
    Behavioral ecology and sociobiology 48 (2000), S. 229-235 
    Details
    ISSN: 1432-0762
    Keywords: Key words Fifteen-spined stickleback ; Spinachia spinachia ; Female choice ; Nest ; Characters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Male fifteen-spined sticklebacks (Spinachia spinachia) build their nests by wrapping epiphytic algae around macroalgae and securing them with secretional threads. In this study, I show that female fifteen-spined stickleback prefer males which have built their nests above the surrounding vegetation. High-located nests are safer, because they are less likely to attract egg-predators such as shore crabs (Carcinus maenas). Thus, I found that shore crabs more rapidly find eggs in nests built close to the bottom than in nests well above the it. Moreover, male-male competition could be an additional explanation as to why males build nests high up. Thus, my field results suggest that the closer the males were to a neighbour, the higher they built. Larger males were also found to have larger territories but male size did not correlate with the height of the nest above the surrounding vegetation. Females showed no preference for nest size, regardless of the presence of egg-predators. However, they tended to choose nests that had no egg-predators in the vicinity over nests with egg-predators close by. Female choosiness for certain nest characters may provide one explanation for the evolution of male care in this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002650000230
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  • 10
    Electronic Resource
    Electronic Resource
    Aggregated Estimation of the Basic Parameters of Biological Production and the Carbon Budget of Russian Terrestrial Ecosystems: 1. Stocks of Plant Organic Mass (2000)
    Springer
    Russian journal of ecology 31 (2000), S. 371-378 
    Details
    ISSN: 1608-3334
    Keywords: biological production ; phytomass stock and density ; forest ecosystems ; bogs ; grasslands and brushwoods ; farmlands ; carbon stock
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The data presented were obtained at the first stage (1993–1999) of studies on evaluating the basic parameters of biological production in Russian terrestrial ecosystems in order to provide information for assessing and modeling the carbon budget of the entire terrestrial biota of the country. Stocks of phytomass (by fractions), coarse woody debris, and dead roots (underground necromass) were calculated by two independent methods, which yielded close results. The total amount of phytomass in Russian terrestrial ecosystems was estimated at 81 800 Tg (=1012 g = million t) dry matter, or 39 989 Tg carbon. Forest ecosystems comprise a greater part (82.1%) of live plant organic matter (here and below, comparisons are made with respect to the carbon content); natural grasslands and brushwoods account for 8.8%; the phytomass of wetlands (bogs and swamps), for 6.6%; and the phytomass of farmlands, for only 2.5%. Aboveground wood contains approximately two-thirds of the plant carbon (63.8%), and green parts contain 9.9%. For all classes of ecosystems, the proportion of underground phytomass averages 26.7% of the total amount, varying from 22.0% in forests to 57.1% in grasslands and brushwoods. The average phytomass density on lands covered with vegetation (1629.9 million hectares in Russia) is 5.02 kg/m2 dry matter, or 2.45 kg C/m2. The total amount of carbon in coarse woody debris is 4955 Tg C, and 9180 Tg C are in the underground necromass. In total, the vegetation of Russian terrestrial ecosystems (without litter) contains 54 124 Tg carbon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026631523999
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