ISSN:
1437-1596
Keywords:
Key words Isolated exclusion
;
Null allele
;
Orosomucoid gene
;
Paternity case
;
Reverse
;
homozygosity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Law
Notes:
Abstract In a German paternity test, an alleged father was excluded only by reverse homozygosity of ORM1 phenotypes (mother ORM1 S, child ORM1 S and alleged father ORM1 F1) out of the 28 classical and DNA markers investigated. Without the ORM1 system the biostatistical probability of paternity was calculated to exceed 99.9999%. The intensity of the immunoprinted bands of the ORM1 protein for the child and alleged father after isoelectric focusing appeared to be reduced to about half. To identify a possible null allele, gene-specific amplification followed by single-strand conformation polymorphism and sequencing analyses were carried out. Deletion of one of the two copies of a 4 bp direct repeat sequence (GTCT) in exon 4 of the consensus sequence of ORM1*F1 was observed in the child and alleged father. Thus, the sharing of a rare mutant gene, ORM1*Q0köln, increased the probability of paternity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004149900118
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