ISSN:
1432-1459
Keywords:
Cerebral Spinal Fluid
;
Immunofluorescence
;
Cell Culture in CSF
;
Mumps-Meningoencephalitis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The cell content of a CSF sample of a patient with mumps meningoencephalitis was divided into 4 portions and cultured in vitro. The first two cultures were stained 24 hrs later with fluorescein conjugated sera by the indirect method. The cells brought together with the serum containing antibodies against mumps virus showed a bright yellowish fluorescence; the controls were free. The diagnosis of mumps meningoencephalitis could be confirmed by serologic methods and by virus isolation. The two remaining cultures were stained 2 weeks later in the same way. A monolayer of epithelial cells had grown out. By fluorescence it could be demonstrated that these cells still contained large amounts of virus material.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00316166
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