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  • 2000-2004  (2)
  • 1970-1974  (3)
  • 1870-1879
  • Chemical Engineering  (3)
  • HIV-1  (1)
  • Keywords Diabetes, insulin, catecholamines, α2-agonist, adrenoceptor, islets, transgenic mice.  (1)
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  • 1
    ISSN: 1432-0428
    Schlagwort(e): Keywords Diabetes, insulin, catecholamines, α2-agonist, adrenoceptor, islets, transgenic mice.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Aims/hypothesis. To study the role of the human α2A-adrenoceptor in the regulation of insulin secretion and the maintenance of glucose homeostasis in transgenic mice overexpressing this receptor in pancreatic beta cells.¶Methods. A human insulin promoter/human α2C10-adrenoceptor chimeric gene was microinjected into mouse embryos and transgenic mice were obtained.¶Results. Analysis by RT-PCR showed that the expression of the transgene was restricted to pancreatic islets. Study of the binding of the α2-antagonist [3H]RX821 002 to membrane preparations showed that islets from transgenic mice had ninefold higher α2-adrenoceptor density than those from controls. Immunohistological analysis showed, however, no change in the number or size of islets between control and transgenic mice. Transgenic animals had normal glycaemia and insulinaemia in basal conditions but greater hyperglycaemic and hypoinsulinaemic responses after injection of the α2-agonist, UK14 304. The lower blood insulin concentration detected in transgenic mice was a reflection of a stronger inhibitory effect of the α2-agonist on glucose-stimulated insulin secretion in transgenic islets than in controls. Furthermore, transgenic mice did not have lower glycaemia to basal values after an intraperitoneal glucose tolerance test. This defect was abolished by treatment with the α2-adrenoceptor antagonist, RX821 002.¶Conclusion/interpretation. These results provide evidence in vivo that overexpression of α2-adrenoceptors in beta cells can lead to impaired insulin secretion and glucose intolerance. [Diabetologia (2000) 43: 899–906]
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1573-2592
    Schlagwort(e): HIV-1 ; immune reconstitution ; heat-shock proteins ; lymphoproliferation ; intracellular cytokines
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Immunologic parameters, known to be grossly abnormal in HIV-1-infected subjects, were analyzed in 22 patients with sustained viral load suppression (〈200 copies/ml) following long-term highly active antiretroviral therapy (HAART). Responses were compared with those from 18 HIV-seronegative healthy controls. Persistent phenotypic alterations in patients' blood mononuclear cells were minimal, though the percentages of lymphocytes that could be activated to produce interleukin-2 (IL-2) remained severely depressed. Using lymphoproliferative assays, a striking deficit in the capacity of patients to respond to the common mycobacterial antigens and particularly to recombinant heat-shock proteins paralleled the absence of responses to virus p24 antigen. In view of the important immunoregulatory role of stress proteins, these findings reveal profound functional deficiencies and persistent immune dysregulation in HIV-1 patients, despite successful HAART and a considerable recovery of CD4+ lymphocyte numbers. Rational immunotherapeutic approaches should be aimed to correct the characterized immune abnormalities.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 20 (1974), S. 981-988 
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The linear stability of thermally stratified horizontal two-phase Couette flow is analyzed for the case of a constant vertical temperature gradient. Instabilities driven by buoyancy, surface tension gradients, or shear are allowed for. It is shown that the instability can take three possible forms: streamwise oriented roll vortices, long interfacial waves, and short Tollmien-Schlichting waves. It is shown that the stability limits for rolls are identical to those for plane, stagnant layers. A long wave expansion is presented and the stability limits for this mode are given algebraically. The nonexistence of a Squire's Theorem is demonstrated and some numerical experiments at moderate Reynolds numbers are described. Detailed comparisons with previous work are possible for only one fluid pair, but it is shown that reasonably accurate statements may be made to determine which mode may manifest itself in any given experimental situation.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 20 (1974), S. 1161-1167 
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The method of energy is used to determine global stability limits for thermally stratified two-phase plane Couette flow. Instabilities due to surface tension variations, buoyancy effects, and shear are allowed for, but surface waves are specifically excluded from consideration. Under these assumptions, the Marangoni, Rayleigh, and Reynolds numbers completely describe the system. Stability plots, valid for disturbances of any magnitude, are presented for both streamwise oriented roll vortices and two-dimensional transversely oriented disturbances. It is shown that rolls are the most dangerous disturbances in the sense that they cannot be shown to be stable relative to transverse disturbances at any nonzero Reynolds numbers. Comparisons are made with existing linear limits, and these are seen to be close only for moderate Reynolds numbers.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 16 (1970), S. 496-498 
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Zusätzliches Material: 3 Tab.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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