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  • 2000-2004  (2)
  • 1970-1974  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    The transmembrane protein occludin of epithelial tight junctions is a functional target for serine peptidases from faecal pellets of Dermatophagoides pteronyssinus (2001)
    Oxford, UK : Blackwell Science, Ltd
    Clinical & experimental allergy 31 (2001), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: There have been only a few studies of how allergens cross the airway epithelium to cause allergic sensitization. House dust mite fecal pellets (HDMFP) contain several proteolytic enzymes. Group 1 allergens are cysteine peptidases, whilst those of groups 3, 6 and 9 have catalytic sites indicative of enzymes that mechanistically behave as serine peptidases. We have previously shown that the group 1 allergen Der p 1 leads to cleavage of tight junctions (TJs), allowing allergen delivery to antigen presenting cells.In this study we determined whether HDMFP serine peptidases similarly compromise the airway epithelium by attacking TJs, desmosomes and adherens junctions.Experiments were performed in monolayers of MDCK, Calu-3 or 16HBE14o-epithelial cells. Cell junction morphology was examined by 2-photon molecular excitation microscopy and digital image analysis. Barrier function was measured as mannitol permeability. Cleavage of cell adhesion proteins was studied by immunoblotting and mass spectrometry.HDMFP serine peptidases led to a progressive cleavage of TJs and increased epithelial permeability. Desmosomal puncta became more concentrated. Cleavage of TJs involved proteolysis of the TJ proteins, occludin and ZO-1. This was associated with activation of intracellular proteolysis of ZO-1. In contrast to occludin, E-cadherin of adherens junctions was cleaved less extensively. Although Calu-3 and 16HBE14o-cells expressed tethered ligand receptors for serine peptidases, these were not responsible for transducing the changes in TJs.HDMFP serine peptidases cause cleavage of TJs. This study identifies a second general class of HDM peptidase capable of increasing epithelial permeability and thereby creating conditions that would favour transepithelial delivery of allergens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2001.00970.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The immunobiology of allergenic peptidases (2003)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 33 (2003), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2003.01601.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Heat capacities of Ar, Ne,4He-Ar, and4He-Ne films adsorbed on Cu at low temperatures (1971)
    Springer
    Journal of low temperature physics 5 (1971), S. 1-16 
    Details
    ISSN: 1573-7357
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Heat capacities of Ar and Ne monolayers adsorbed on Cu are measurable at 1–4 K. Temperature dependences are similar to the low-temperature law for harmonic oscillators, but the empirical characteristic temperatures of 11–15 K are much lower than can correspond to independent adatoms oscillating in substrate sites. The magnitudes and temperature dependences do not match those predicted for surface modes of an absorbent coated with inert impurities or for the collective modes of epitaxial films. A model involving the excitations in non-epitaxial films appears to offer an explanation of the observed magnitudes and temperature dependences. The heat capacities of4He adsorbed on preplated Ar or Ne show little variation with the number of Ar layers, or between Ar and Ne coatings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00628225
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Heat capacities of submonolayer He3 and He4 adsorbed on Ar-plated copper (1970)
    Springer
    Journal of low temperature physics 3 (1970), S. 281-281 
    Details
    ISSN: 1573-7357
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00628152
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    Paper (German National Licenses)
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