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  • 1
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: During raspberry (Rubus ideaus L. cv Glen Clova) fruit ripening, endo-β-1,4-glucanase (EGase; EC 3.2.1.4) specific activity (per g fresh weight) increases approximately 15-fold. Highest activity was associated with the surfaces of the receptacles where the weakening abscission zones are located. Immunoblotting using antibodies raised against a bean abscission-EGase (ab.-EGase) identified a single protein of Mr 52 kDa that was present only in ripe fruit and was most prominent in the receptacle. Using reverse transcription-polymerase chain reaction (RT-PCR), two 497-bp partial putative EGase clones were obtained from ripe receptacle mRNA (termed RI-EGL1 and 2) which share 53% amino acid identity. The more abundant RI-EGL1 was used to obtain its full length clone from a ripe receptacle cDNA library. The deduced amino acid sequence of RI-EGL1 was similar to other ab.-EGase sequences (ca 67%) and contained the conserved motifs present in all E2-class EGases. Northern analysis revealed that RI-EGL1 expression was limited to ripe-fruit receptacles. Application of the ethylene antagonist 1-methylcyclopropene (1-MCP) to green fruits indicated that endogenous ethylene accelerates raspberry abscission and increases both EGase activity and RI-EGL1 expression.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Aequationes mathematicae 9 (1973), S. 23-29 
    ISSN: 1420-8903
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Aequationes mathematicae 60 (2000), S. 283-290 
    ISSN: 1420-8903
    Keywords: Keywords. Aggregation equation, generalized bisymmetry, injective.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Summary. It is shown that provided F and G are injective in every argument, the functional equation of generalized $ m \times n $ bisymmetry $ (m,n \ge 2) $ ,¶¶ $ G(F_1(x_{11}, \hdots , x_{1n}),\hdots , F_m(x_{m1},\hdots, x_{mn})) $ ¶ $ = F(G_1(x_{11},\hdots , x_{m1}),\hdots , G_n(x_{1n},\hdots , x_{mn})) $ ¶may be reduced to ¶¶ $ G(\overline{F}_1(u_{11}, \hdots , u_{1n}),\hdots , \overline{F}_m(u_{m1},\hdots, u_{mn})) $ ¶ $ = F(\overline{G}_1(u_{11},\hdots , u_{m1}),\hdots ,\overline{G}_n(u_{1n},\hdots , u_{mn})) $ ¶where¶¶ $ F_i(x_{i1},\hdots , x_{in}) = \overline{F}_i (\varphi_{i1}(x_{i1}),\hdots , \varphi_{in}(x_{in})), G_j(x_{1j}, \hdots , x_{mj}) = \overline{G}_j(\varphi_{1j} (x_{1j}),\hdots, \varphi_{mj}(x_{mj})) $ ,¶¶ $ \varphi_{ij} are surjections and $ \overline{F}_i, \overline{G}_j $ are injective in every argument for all $ 1\le i \le m,\ 1\le j\le n $ . The result is also shown to hold for a wider class of functional equations.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Aequationes mathematicae 11 (1974), S. 318-319 
    ISSN: 1420-8903
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2048
    Keywords: Key words: Gene expression (fruit) – Fruit ripening – RNA-fingerprinting –Rubus (fruit ripening)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Processes that contribute to the overall phenomenon of fruit ripening are not well understood for many soft fruit species, including raspberry (Rubus idaeus L.) Recent biochemical data implicate ethylene and a range of cell wall hydrolases in ripening processes. However, the genes encoding these activities, and others related to ripening, remain to be characterised. With the advent of high-throughput RNA-fingerprinting techniques it is possible to characterise rapidly the changes in gene expression during developmental processes. This paper describes the application of two RNA-fingerprinting techniques (cDNA amplified fragment length polymorphism and differential display reverse transcription-polymerase chain reaction) to the ripening fruit of Rubusi daeus. Copy-DNA tags were isolated representing 34 genes, up-regulated during fruit ripening. The expression profiles of these genes were determined by RNA-blot analysis and their sequences were compared with those in public databases. Potential roles for some of these gene products are considered, providing valuable insights into the processes that underpin fruit ripening. Many of the cDNAs isolated in this study provide tools for the biotechnological improvement of fruit quality.
    Type of Medium: Electronic Resource
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