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  • 2000-2004  (5)
  • 1955-1959
  • 2-phenylethyl glucosinolate  (1)
  • Cancer  (1)
  • Colorectal cancer  (1)
  • Cost-effectiveness  (1)
  • DNA repair genes  (1)
Source
Material
Years
  • 2000-2004  (5)
  • 1955-1959
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Molecular staging of colorectal cancer (2000)
    Springer
    Diseases of the colon & rectum 43 (2000), S. 155-159 
    Details
    ISSN: 1530-0358
    Keywords: Colorectal cancer ; Micrometastasis ; Staging ; K-ras ; Lymph nodes ; AS-PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract PURPOSE. Multiple attempts have been made to improve the clinical/pathologic staging system of Dukes to focus adjuvant therapy decisions. The purpose of this study was to determine whetherK-ras mutational status of regional nodes in patients with Dukes B2 colorectal cancer could be used to stage their disease more accurately. METHODS: Using formalin-fixed, paraffin-embedded archival material, tumor samples were screened forK-ras mutations using a mutation-specific polymerase chain reaction method, followed by gel electrophoresis in a 96-well array. Patients with Dukes B2 tumors that have mutations in codon 12 or 13 of theK-ras gene were identified. RESULTS: Mutational analysis of the lymph nodes from these patients revealed an 80 percent (16/20) incidence of the same mutations in regional lymph nodes. None of the four patients with mutation-free nodes developed recurrence compared with 37.5 percent (6/16) withK-ras positive lymph nodes. CONCLUSIONS: The data suggest that patients with Dukes B2 colorectal cancers that have mutations in codon 12 or 13 of theK-ras gene are at high risk for the development of nodal metastases. Mutational analysis of the lymph nodes identifies high-risk patients who should be considered for adjuvant chemotherapy. Therefore,K-ras mutational analysis should be considered for molecular staging of colorectal cancer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02236973
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Defective DNA repair genes in a primary culture of human renal cell carcinoma (2000)
    Springer
    Journal of cancer research and clinical oncology 126 (2000), S. 185-190 
    Details
    ISSN: 1432-1335
    Keywords: Key words Renal cell carcinoma ; DNA repair genes ; Microsatellite stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose: Genomic stability is maintained by error-free DNA replication, repair, and recombination. To determine if repair genes contribute to genomic instability, we used a newly established cell line RCC-AJR (from clear-cell renal cell carcinoma) to examine hMSH2 (a mismatch-repair gene) and the gene encoding DNA β polymerase (polβ; a known contributor to base-excision repair). Methods: Coding sequences of hMSH2 and polβ were amplified by the polymerase chain reaction (PCR) using RNA from RCC-AJR cells and matched normal kidney (NK) cells from the same patient. Nucleotide sequences of the PCR products were determined by the dideoxy-DNA method and direct sequencing. Expressions of repair genes were assayed by Western blotting. Microsatellite stability in RCC-AJR cells was assayed by alteration in (CA) n repeats. Results: In the RCC-AJR cells, we detected (a) a deletion of 1476 bp encoding 492 amino acids of hMSH2 cDNA, (b) an 87-bp deletion in the polβ coding sequence, (c) truncated forms of hMSH2 and polβ proteins, and (d) microsatellite instability. Conclusions: This study provides evidence of alterations in hMSH2 and polβ in the homogeneous cell population of an RCC-AJR tumor culture. The data indicate that repair genes may help preserve genomic stability in this cell line. We believe that this new primary RCC-AJR cell line will prove a useful model for investigating the cascade of genetic events in renal cells that leads to renal carcinogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004320050031
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Helical CT in the primary trauma evaluation of the cervical spine: an evidence-based approach (2000)
    Springer
    Skeletal radiology 29 (2000), S. 632-639 
    Details
    ISSN: 1432-2161
    Keywords: Keywords Cervical spine screening ; CT scan ; Trauma ; Cost-effectiveness ; Cervical spine fracture ; Clinical predictors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  This review provides a summary of the cost-effectiveness, clinical utility, performance, and interpretation of screening helical cervical spine CT for trauma patients. Recent evidence supports the use of helical CT as a cost-effective method for screening the cervical spine in high-risk trauma patients. Screening cervical spine CT can be performed at the time of head CT to lower the cost of the evaluation, and when all short- and long-term costs are considered, CT may actually save money when compared with traditional radiographic screening. In addition to having higher sensitivity and specificity for cervical spine injury, CT screening also allows more rapid radiological clearance of the cervical spine than radiography. Patients who are involved in high-energy trauma, who sustain head injury, or who have neurological deficits are candidates for CT screening. Screening with CT may enhance detection of other potentially important injuries of the cervical region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560000270
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Breeding to Increase the Concentration of 2-phenylethyl Glucosinolate in the Roots of Brassica napus (2000)
    Springer
    Journal of chemical ecology 26 (2000), S. 1811-1820 
    Details
    ISSN: 1573-1561
    Keywords: Brassica napus ; Pratylenchus neglectus ; nematode ; 2-phenylethyl glucosinolate ; isothiocyanate ; pest resistance ; disease break ; variability ; genetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Root concentrations of 2-phenylethyl glucosinolate in canola, Brassica napus, influence the susceptibility of the crop to the root lesion nematode (Pratylenchus neglectus), as well as the nematicidal effect of root tissues as they degrade in the soil. Plants containing high 2-phenylethyl glucosinolate should therefore reduce soil populations of P. neglectus. A selection program was developed to increase the proportion of total glucosinolates contributed by 2-phenylethyl glucosinolate in the roots of B. napus cv. Dunkeld. Variation within this accession was observed to be stable across the S1 and S2 generations. The segregation observed for 2-phenylethyl glucosinolate percentage suggested that the trait was encoded at a single locus, with the "high" phenotype being dominant. Plants with the high 2-phenylethyl glucosinolate phenotype (〉45% of total glucosinolates) were shown to be significantly more resistant to P. neglectus than otherwise identical "low" phenotypes (〈45% of total).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005588405774
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Perception of quality of life by patients, partners and treating physicians (2000)
    Springer
    Quality of life research 9 (2000), S. 1041-1052 
    Details
    ISSN: 1573-2649
    Keywords: Cancer ; Proxy ; Quality of life
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The objective of the study was to determine possible differences in perception of quality of life (QoL) between patients with metastatic breast or prostate cancer, their partners, and the treating physician. Patients with metastatic breast cancer (n = 71), and metastatic prostate cancer (n = 29), a partner, and the physician each completed the same QoL questionnaire indicating how they perceived the patient's QoL. The European organization for research and treatment of cancer (EORTC) QLQ-C30 questionnaire was used to assess patients with breast cancer and the modified prostate cancer specific quality of life instrument (PROSQOLI) for patients with prostate cancer. There was reasonable agreement in mean scores between patients, and physicians or partners, for many domains of QoL; however, there was substantial discordance between scores when considering individual patients. For patients with metastatic breast cancer, physicians systematically underestimated overall QoL (p = 0.0002), social functioning (p = 0.001), and role functioning (p = 0.008), while partners showed better agreement. With prostate cancer physicians tended to underestimate pain, while mean scores for spouses were more concordant. There is substantial variability between ratings of QoL by physicians or partners, as compared to patient ratings. Medical decisions should be based on information about QoL provided by patients using validated methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1016647407161
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    Paper (German National Licenses)
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