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  • 2000-2004  (4)
  • 1955-1959
  • pregnancy rate  (2)
  • AS-PCR  (1)
  • Electromyogram  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Diseases of the colon & rectum 43 (2000), S. 155-159 
    ISSN: 1530-0358
    Schlagwort(e): Colorectal cancer ; Micrometastasis ; Staging ; K-ras ; Lymph nodes ; AS-PCR
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract PURPOSE. Multiple attempts have been made to improve the clinical/pathologic staging system of Dukes to focus adjuvant therapy decisions. The purpose of this study was to determine whetherK-ras mutational status of regional nodes in patients with Dukes B2 colorectal cancer could be used to stage their disease more accurately. METHODS: Using formalin-fixed, paraffin-embedded archival material, tumor samples were screened forK-ras mutations using a mutation-specific polymerase chain reaction method, followed by gel electrophoresis in a 96-well array. Patients with Dukes B2 tumors that have mutations in codon 12 or 13 of theK-ras gene were identified. RESULTS: Mutational analysis of the lymph nodes from these patients revealed an 80 percent (16/20) incidence of the same mutations in regional lymph nodes. None of the four patients with mutation-free nodes developed recurrence compared with 37.5 percent (6/16) withK-ras positive lymph nodes. CONCLUSIONS: The data suggest that patients with Dukes B2 colorectal cancers that have mutations in codon 12 or 13 of theK-ras gene are at high risk for the development of nodal metastases. Mutational analysis of the lymph nodes identifies high-risk patients who should be considered for adjuvant chemotherapy. Therefore,K-ras mutational analysis should be considered for molecular staging of colorectal cancer.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1439-6327
    Schlagwort(e): Key words Muscle wisdom ; Fatigue ; Muscular compliance ; Mechanomyogram ; Electromyogram
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The purpose of this study was to examine the mechanomyographic (MMG) and electromyographic (EMG) responses during continuous, cycle ergometer workbouts performed at constant power outputs. Eight adults [mean (SD) age, 21.5 (1.6) years] volunteered to perform an incremental test to exhaustion for the determination of peak power (W˙ peak) and four, 15-min (or to exhaustion) rides at constant power outputs of 50%, 65%, 80%, and 95% W˙ peak. Piezoelectric crystal contact sensors were placed on the vastus lateralis (VL) and vastus medialis (VM) muscles to record the MMG signals. Bipolar surface electrode arrangements were placed on the VL and VM to record the EMG signals. Five-second samples of the MMG and EMG signals were recorded every 30 s at power outputs of 50%, 65%, and 80% W˙ peak, and every 15 s at 95% W˙ peak. The amplitudes of the selected portions of the signals were normalized to the first values recorded during the continuous rides, and regression analyses were used to determine whether the slope coefficients for the MMG and EMG versus time relationships were significantly (P 〈 0.05) different from zero. The results indicate that EMG amplitude increased (range of slope coefficients: 0.03–0.56) during the continuous rides for both muscles at all four power outputs (except the VM at 50% W˙ peak), while MMG amplitude increased (slope coefficient at 95% W˙ peak for VM=0.19), decreased (range of slope coefficients for VL and VM at 50% and 65% W˙ peak=−0.14 to −0.24), or remained unchanged (range of slope coefficients for VL and VM at 80% W˙ peak and VL at 95% W˙ peak=−0.06 to 0.12) depending on the power output. The patterns of the MMG responses, however, were similar for the VL and VM muscles, except at 95% W˙ peak. Fatigue-induced changes in motor-unit recruitment and discharge rates, or muscular compliance may explain the differences between power outputs in the patterns of the MMG amplitude responses.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1573-7330
    Schlagwort(e): infertility—male ; infertility—female ; insemination—martificial ; sperm motility ; pregnancy rate
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Purpose: To identify characteristics of female patients andof semen that were associated with live birth followingintra-uterine insemination (IUI). Methods: Retrospective review of medical and laboratoryresults from 533 women who underwent IUI with partner'ssperm from 1993 through 1995. Results: Among 1728 cycles, 116 (6.7%) resulted in livedeliveries. Among the 38 patient and semen variablesanalyzed, only 3 were associated with successful IUI outcome:female age 〈37.7 years at the time of treatment (P = 0.02);the absence of any corrective pelvic surgery (P 〈 0.001);and postwash sperm motility (P = 0.006). Couples withnone of these three risk factors achieved per-cycle pregnancyrates of 12.4%. Women with two risk factors (age and pelvicsurgery) achieved per-cycle pregnancy rates of 4.6% whensperm had good postwash motility. No pregnancies wereachieved when low postwash motility was combined withany other risk factor. Conclusions: Advanced female age, poor postwash spermmotility, and a history of corrective pelvic surgery aresignificant risk factors for poor IUI success rates. Poor postwashsperm motility in combination with either of these other tworisk factors resulted in no successful pregnancies.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    ISSN: 1573-7330
    Schlagwort(e): infertility-female ; ovulation induction ; pregnancy rate ; insemination-artificial ; sperm motility
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Purpose: We measured the effect of ovulatory stimulation(OS) upon intrauterine insemination (IUI) success rates,particularly among patients with risk factors for IUI failure. Methods: Retrospective review of medical and laboratoryresults from 512 patients who underwent 1576 cycles ofIUI with partner's sperm over a 3 year period. Data werecollected on 3 risk factors for IUI failure (advanced femaleage 〉37.7 years, prior corrective pelvic surgery, and poorpost-wash sperm motility), and on method of OS (none,clomiphene citrate, or gonadotropin). Results: Patients who underwent OS had significantly higherpregnancy rates (7.6;pc) than those who did not (4.7%, p =0.02). However, when patients were stratified by their riskfactors, OS made a significant difference only for patientswithout risk factors. These patients had a 15.5% per cyclepregnancy rate with OS, compared to 7.9% in unstimulatedIUI cycles (p = 0.04). Conclusions: Ovulatory stimulation doubles IUI pregnancyrates among young patients without a prior pelvic surgeryand with good post-wash semen quality. The benefit of OSfor patients with risk factors for IUI failure is unclear. Thesepatients should be counseled that their chances for successwith IUI are limited, with or without OS.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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