ISSN:
1615-2573
Keywords:
Key words Nicorandil
;
Adenosine
;
Ecto-5′-nucleotidase
;
Cyclic guanosine monophosphate
;
Microdialysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract With the use of microdialysis techniques, we examined the effects of nicorandil, a hybrid of an ATP-sensitive K+ (KATP) channel opener and a nitrate compound, on the production of interstitial adenosine in rat hearts in situ. The level of dialysate adenosine measured under a constant supply of adenosine 5′-monophosphate (AMP) reflected the activity of endogenous ecto-5′-nucleotidase. Nicorandil (0.3–3 mM) increased the level of AMP (100 μM)-primed dialysate adenosine in a concentration-dependent manner, and this effect was completely abolished by the guanylate cyclase inhibitor, methylene blue (100 μM), but not by the KATP channel blocker, glibenclamide (10 μM). Another KATP channel opener, cromakalim (0.1–1 mM), did not increase the production of AMP-primed dialysate adenosine. These results suggest that nicorandil increases the level of interstitial adenosine via cyclic guanosine monophosphate-mediated activation of ecto-5′-nucleotidase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s003800070036
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