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  • 2000-2004  (3)
  • 1920-1924
  • GRGDS  (1)
  • Head and Neck Squamous Cell Carcinoma  (1)
  • Macrobrachium nipponense  (1)
Source
Material
Years
  • 2000-2004  (3)
  • 1920-1924
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Effect of pH and Zn2+ on subcultured muscle cells from Macrobrachium nipponense (2000)
    Springer
    Methods in cell science 22 (2000), S. 277-284 
    Details
    ISSN: 1573-0603
    Keywords: Macrobrachium nipponense ; Cell subculture ; pH ; Zn2+
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cell culture system was devised for muscle cell of Macrobrachium nipponense in the study. The juvenile and adult shrimps were held in laboratory aquaria with penicillin 1000 IU/ml and streptomycin 1000 µg/ml for 12–24 hours. Cell cultures were established in medium 199 supplemented with 20% fetal bovine serum, 1 g/L glucose, 5.2 g/L NaCl, 1.43 g/L CaCl2, 0.05 g/L MgCl2, 100 IU/mL penicillin and 100 µg/ml streptomycin. Fibroblast-like cells were passaged up to three times and survived for 54 days. The results showed the optimum for subculture in vitro was in medium 199 with pH 7.6. Moreover, basal medium supplemented with Zn2+ 60 µg/L could enhance the growth of the muscle cells. It was found that better results for cell culture would be obtained more easily with juvenile shrimps caught in spring than adults in summer or autumn; and shrimps caught within 12 hours after ecdysis could grow much better than the intermoult shrimps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1017962429862
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Inactivation of p16INK4a in Primary Tumors and Cell Lines of Head and Neck Squamous Cell Carcinoma (2000)
    Springer
    Molecules and cells 10 (2000), S. 557-565 
    Details
    ISSN: 0219-1032
    Keywords: DNA Methylation ; Head and Neck Squamous Cell Carcinoma ; p16INK4a
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Inactivation of the p16INK4a gene by mutation and deletion is common in head and neck squamous cell carcinoma (HNSCC). The present study demonstrates that hypermethylation of the 5′ CpG islands can serve as an alternative mechanism for the inactivation of the p16INK4a gene in this tumor. We studied 11 HNSCC cell lines and 17 oral squamous cell carcinoma (OSCC) primary tumors for p16INK4a gene status by protein/mRNA and DNA genetic/epigenetic analyses to determine the incidence of its inactivation. Our study indicates that: (1) inactivation of p16 protein is frequent in HNSCC cell lines (6/11, 54.5%) and OSCC primary tumors (15/17, 88.2%), (2) inactivation of p16INK4a protein is commonly associated with the presence of gene alteration such as mutation, homozygous deletion and especially aberrant methylation, and (3) genomic sequencing of bisulfite-modified DNA shows that the carcinoma develops a heterogeneous pattern of hypermethylation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s10059-000-0557-8
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Conjugation of Arg-Gly-Asp sequence into thermo-reversible gel as an extracellular matrix for 3T3-L1 fibroblast cell culture (2000)
    Springer
    Biotechnology letters 22 (2000), S. 1553-1556 
    Details
    ISSN: 1573-6776
    Keywords: fibroblast cell ; gel ; GRGDS ; integrin family ; N-isopropylacrylamide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract High molecular weight N-isopropylacrylamide copolymers with small amounts of acrylic acid (typically 2–5 mol% in feed) were synthesized by free radical polymerization in benzene and then conjugated with adhesion molecules of Gly-Arg-Gly-Asp-Ser (GRGDS) peptides. Aqueous polymer solutions (5, 6, 8 and 10% w/v) in culture medium (pH 7.4, ionic strength; 0.15 M) with 3T3-L1 fibroblast cells were mixed and poured in Millicells, which supported the gel formation without a significant gel induction time at 36 °C (gelation temperature). The initially formed gel was translucent and became more opaque as the temperature increased. The interaction between fibroblast cells and an artificial matrix of GRGDS containing p(NiPAAm-co-AAc) copolymer gel resulted in effective cell attachment, proliferation and growth. This study supported that specific attachment is the result of the interaction between the integrin families on the fibroblast and the RGD sequence on the p(NiPAAm-co-AAc) copolymer gel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005680800420
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    Paper (German National Licenses)
    Fulltext
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