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  • 2000-2004  (4)
  • 1915-1919
  • Dendritic cells  (2)
  • Keywords  (2)
  • 1
    ISSN: 1432-2277
    Keywords: Key words Rat liver transplantation ; Intrahepatic leukocytes ; Dendritic cells ; T lymphocytes ; Chimerism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Long-term graft acceptance and tolerance induction after allogeneic rat liver transplantation are well described. However, the underlying mechanisms remain unclear. In this study we investigated the cellular events within the liver graft during initial immunosuppression and long-term acceptance. Orthotopic liver transplantation was performed in the Dark Agouti (DA)-to-Lewis (LEW) and LEW-to-DA rat strain combination. In order to achieve long-term acceptance, LEW recipients of DA livers were treated with two different short-term therapies. Non-parenchymal cells (NPC) were isolated from liver allografts on days + 10 and + 100 after transplantation and donor-specific leukocytes were immunophenotyped by flow cytometry. Both the monotherapy and triple therapy prolonged graft survival (〉 100 days). Liver allografts from LEW donors into DA recipients were spontaneously accepted across a complete MHC mismatch without immunosuppression. Liver allograft rejection was induced by infiltrating alloreactive immunocompetent cells. But the intensities of cell infiltration in the early and late phases after transplantation did not correlate with eventual outcome. Donor-specific NPC decreased to 18–25 % on day + 10 in both therapeutic groups, but had rebounded to up to 40 % by day + 100. Recurrence of donor-specific cells was caused almost exclusively by rising T cell counts. The persistence of dendritic cells in the late phase after transplantation could be clearly demonstrated. Repopulation by donor-specific T lymphocytes was observed in long-term accepted liver grafts. This recurrence may be based on the differentiation of liver-derived progenitor cells. The persistent coexistence of donor and recipient cells within the liver allograft (intrahepatic chimerism) appears to be characteristic and may be important for long-term acceptance.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1436-6274
    Keywords: Schlüsselwörter ; Ventrale Spondylodese ; Funktionelles ¶Ergebnis ; Untere Halswirbelsäule ; Keywords ; Ventral spondylodesis ; Functional results ; Low cervical spine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: A total of 58 patients were examined to determine the functional results in the cervical spine an average of 5.3 years after ventral spondylodesis. After questioning and physical examination the function was assessed by functional radiographs (according to Penning) and dynamic 3-D motion analysis. In 54% of cases the patients reported no subjective restrictions of their mobility or only slight restrictions, while 22% experienced moderate to marked limitations in their professional life and 28%, in their activities of daily living, but 84% of the patients were nonetheless still engaging in the same types of sport as before. The clinical examination revealed the following ranges of movement (MV ± SD) in the cervical spine: anteflexion 37°± 19°; retroflexion 46°± 21°; lateral bending 49°± 24°; and rotation in neutral position 112°± 42°. The functional radiographs showed smaller values: anteflexion 24.2°± 11°; and retroflexion 25.4°± 14°. The 3-D motion analysis confirmed a restriction compared with the normal collective: 20% in the sagittal plane, 13% in the frontal plane, 18% with rotation in the neutral position, 15% in maximal anteflexion and 26% in maximal retroflexion of the cervical spine. The function of the cervical spine is independent of age, sex and extent of the spinal fusion; restrictions tend to be more pronounced with increasingly caudally located spondylodeses. Compensatory hypermobility of the neighbouring segments cannot generally be confirmed.
    Notes: Durchschnittlich 5,3 Jahre nach ventraler Spondylodese wurden 58 Patienten hinsichtlich des funktionellen Ergebnisses der Halswirbelsäule nachuntersucht. Nach Befragung und klinischer Untersuchung wurde die Funktion mit Hilfe von Funktionsaufnahmen (nach Penning) und dynamischer 3D-Bewegungsanalyse beurteilt. Subjektiv keine oder geringe Einschränkungen der HWS-Beweglichkeit bemerkten 54% der Patienten, im Beruf sind 22% und im Alltag 28% mäßig bis stark eingeschränkt, dennoch üben 84% alte sportliche Aktivitäten aus. Die klinische Untersuchung ergab folgende Bewegungsauslenkungen (MW ± SD): Anteflexion: 37 ± 19°, Retroflexion: 46 ± 21°, bilaterale Seitneigung: 49 ± 24°, Rotation in Neutralstellung: 112 ± 42°. Die Funktionsaufnahmen zeigten geringere Werte: Anteflexion: 24,2 ± 11° und Retroflexion: 25,4 ± 14°. Die 3D-Bewegungsanalyse bestätigte eine Einschränkung gegenüber dem Normalkollektiv: in der Sagittalebene 20%, in der Frontalebene 13%, bei Rotation in der Neutralstellung 18%, in der maximalen Anteflexion 15% und in der maximalen Retroflexion 26%. Die Funktion der HWS war vom Alter, vom Geschlecht und von der Ausdehnung der Fusion unabhängig; kaudaler gelegene Spondylodesen zeigten tendenziell größere Einschränkungen. Kompensatorische Hypermobilitäten der benachbarten Segmente konnten im Regelfall nicht bestätigt werden.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1436-6274
    Keywords: Schlüsselwörter ; Mesenchymale Vorläuferzellen ; CFU-F ; Alkalische Phosphatase ; Klonales Wachstum ; Keywords ; Mesenchymal progenitor cells ; CFU-F ; Alkaline phosphatase ; Clonal growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: In 7 patients undergoing corrective surgery of the limbs, cancellous bone was harvested from the iliac crest. During this procedure bone marrow was aspirated. Mononuclear cells were isolated by density gradient centrifugation, and a clonal growth was initiated for CFU-F. Cultures were kept for 2, 3 and ¶4 weeks. In mean 5.6 × 105 mononuclear cells/ml aspirate were isolated. The average clonal efficiency was 4.5 colonies/ml aspirate. As a sign of osteogenic differentiation 15.3% (2 weeks), 42.2% (3 weeks) and 64.3% (4 weeks) of the areas of clonal growth stained positive for alkaline phosphatase. Immunostaining for osteocalcin showed a similar pattern confirming these results. These results suggest that human mesenchymal progenitors can be isolated from marrow punctures and that with matching culture conditions osteogenic differentiation is possible.
    Notes: Bei Spongiosaentnahmen im Rahmen aseptischer Eingriffe wurde bei 7 Patienten ein Knochenmarkaspirat vom vorderen Beckenkamm gewonnen. Die mononukleäre Zellfraktion wurde über Gradientenzentrifugationen isoliert, und die Zellen wurden in Form eines klonalen Wachstums gezüchtet. Die Kulturzeiten betrugen 2,3 und 4 Wochen. Im Mittel ließen sich 5,6 × 10 5 Zellen/ml Aspirat gewinnen. Die klonale Wachstumseffizienz betrug im Durchschnitt 4,5 Kolonien/ml Aspirat. Als Hinweis auf eine osteogene Differenzierung exprimierten nach ¶2 Wochen 15,3%, nach 3 Wochen 42,2% und nach 4 Wochen 64,3% Flächenanteil der Kolonien alkalische Phosphatase. Dieses Ergebnis konnte durch immunzytochemische Färbung auf Osteokalzin bestätigt werden. Die Ergebnisse zeigen, dass humane mesenchymale Vorläuferzellen aus dem Knochenmark isoliert und unter den passenden Kulturbedingungen in die osteogene Reihe differenziert werden können.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2277
    Keywords: Key words Liver/small bowel transplantation ; Tolerance ; Dendritic cells ; Kupffer cells ; Antigen-presenting cells ; Chimerism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The predictive value of chimerism was evaluated in three different transplantation models in the rat without immunosuppression: small bowel- (SBTx), liver- (LTx), and liver/small bowel transplantation (LSBTx) were performed in the Brown Norway (BN)-to-Lewis- (LEW) strain combination. Immunohistochemistry and flow cytometry were used to identify donor cells in the recipient's spleen. Their number did not change significantly during transient rejection or tolerance after LTx and LSBTx. However, the amount of donor-derived nonparenchymal cells within the liver allograft including antigen-presenting cells (APCs), such as dendritic and Kupffer cells, clearly mirrored the recipient's immune status: as expected, their number decreased during rejection, but recovered considerably during and after tolerance induction. We conclude that donor cells in the periphery of the recipient correlate with the presence of the allograft, but do not seem to influence graft acceptance actively. However, the kinetics of the detected donor APC population in the liver suggests their important role in modifying the recipient's immune response towards tolerance.
    Type of Medium: Electronic Resource
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