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  • 1
    Electronic Resource
    Electronic Resource
    Aberration of chromosomes 8 and 11 in bladder cancer as detected by fluorescence in situ hybridization (2000)
    Springer
    Urological research 28 (2000), S. 185-190 
    Details
    ISSN: 1434-0879
    Keywords: Key words Bladder cancer ; FISH method ; Chromosomal abnormality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Although a bladder cancer-specific abnormality in chromosomes or genes has not been reported, chromosomal regions that tend to become abnormal have been recognized. In this study, we investigated abnormalities in chromosomes 8 and 11. There were 27 patients with bladder cancer, 16 males and 11 females, who participated in this study. Abnormalities in chromosomes 8 and 11 were investigated by the fluorescence in situ hybridization (FISH) method. Probes used in this study were chromosome 8α-satellite and chromosome 11α-satellite (Oncor Co.). Of 27 cases, 15 cases were positive for chromosome 8 (55.6%) and ten cases were positive for chromosome 11 (37.0%). Since the FISH method detects chromosomal abnormality by the number of signals generated in cancer cells, this method is objective and simple and thus may be applicable in clinical practice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002409900077
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Rearrangement of cortical microtubules from transverse to oblique or longitudinal in living cells of transgenicArabidopsis thaliana (2000)
    Springer
    Protoplasma 213 (2000), S. 28-38 
    Details
    ISSN: 1615-6102
    Keywords: Arabidopsis thaliana ; Cortical microtubules ; Cytoskeleton ; Green-fluorescent-α-tubulin 6 fusion protein ; Microtubule ; Transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary AGFP-TUA6 (α-tubulin 6) gene was transduced in theArabidopsis thaliana genome, and the GFP-TUA6 protein was expressed by 20% of the total α-tubulin amount. The expressed GFP-TUA6 protein was incorporated into cortical microtubules (cMTs), so that the cMTs could be visualized under the fluorescence microscope in the living cells. The rearrangement of cMTs was observed at the tangential epidermal cell face of the hypocotyl. At the initial stage of light-induced cMT rearrangement from a transverse to an oblique or a longitudinal orientation, randomly oriented short MTs appeared. These MTs rapidly elongated obliquely or longitudinally as the transverse cMTs shortened. Finally, the transverse cMTs were replaced by the newly organized oblique or longitudinal cMTs. Reorganization of the cMTs took 50–70 min. Treatment of seedlings with 5 × 10−5 M cytochalasin B induced disarrayed cMTs. The involvement of cytochalasin B in the orientation of developing MTs is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01280502
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    Paper (German National Licenses)
    Fulltext
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