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  • 2000-2004  (3)
  • Chromosome 20q13.33  (1)
  • D5Mit318  (1)
  • Key words Nitrates  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A case of fatal methemoglobinemia of unknown origin but presumably due to ingestion of nitrate (2000)
    Springer
    International journal of legal medicine 113 (2000), S. 164-167 
    Details
    ISSN: 1437-1596
    Keywords: Key words Nitrates ; Nitrites ; Methemoglobinemia ; GC-NPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract A case of fatal methemoglobinemia (MetHb-emia) which was presumably due to ingestion of nitrate is presented. An unidentified man was taken to a local emergency hospital suffering from deep cyanosis and 7 h later he was found dead in the hospital bed. The post-mortem examination of the blood revealed a methemoglobin (MetHb) concentration of 78% and the concentrations of nitrate and nitrite were 1.50 and 0.76 μg/mL, respectively. Capillary gas chromatography coupled to mass spectrometry (GC-MS) and capillary gas chromatography with a nitrogen-phosphorus detector (NPD) were used to detect nitrates and nitrites in the blood.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004140050290
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  • 2
    Electronic Resource
    Electronic Resource
    cDNA cloning, tissue expression, and chromosome mapping of human homolog of SOX18 (2000)
    Springer
    Journal of human genetics 45 (2000), S. 192-195 
    Details
    ISSN: 1435-232X
    Keywords: Key wordsSOX18 ; HMG-box ; RH mapping ; Chromosome 20q13.33
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The SRY (sex-determining region Y) gene encodes a transcription factor characterized by a DNA-binding motif termed the HMG (high mobility group) domain. The SOX (Sry-box) genes comprise a large family related by homology to the HMG-box region. We isolated a cDNA clone with an open reading frame encoding a putative protein of 384 amino acids, which shared 83% identity to the mouse Sox18 protein. Northern blot analysis revealed that a 1.9-kb band of human SOX18 messenger RNAs was predominantly expressed in heart, although weak signals were seen in brain, liver, testis, and leukocyte. By polymerase chain reaction (PCR)-based analyses with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel, the gene was mapped to the chromosome 20q13.33 region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380050210
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    cDNA cloning, expression profile, and genomic structure of human and mouse RNF10/Rnf 10 genes, encoding a novel RING finger protein (2000)
    Springer
    Journal of human genetics 45 (2000), S. 38-42 
    Details
    ISSN: 1435-232X
    Keywords: Key words RING finger (C3HC4-type zinc finger) motif ; RNF10 ; Rnf10 ; KIAA0262 ; RH mapping ; 12q23–q24.1 ; D5Mit318
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. A new member of the RING finger protein family was identified through a mouse expressed sequence tag (EST) database search, and its full-length cDNA was isolated from a mouse brain full length-enriched cDNA library. The gene was designated as Rnf10, for RING finger protein 10. The cDNA clone consists of 3110 nucleotides and encodes an open reading frame (ORF) of an 804-amino acid protein. A database search revealed that human KIAA0262 protein (accession number, D87451) has strong homology to mouse Rnf10. To confirm that mouse Rnf10 is the homolog or an isolog of human KIAA0262, a human RNF10 cDNA was cloned in our hands from a fetal brain cDNA pool. The newly isolated cDNA contained an ORF for 811 amino acids which had almost identical structure to mouse Rnf10 protein, indicating that the human ORF codes for RNF10 protein. This finding was also supported by comparative chromosome mapping in which both genes were localized in a conserved linkage homology region between mouse and human. Comparison of the RNF10 and KIAA0262 proteins revealed that both were transcribed from the same gene and that the longer RNF10 ORF would be the authentic form. The complete genomic organization of RNF10 was determined to consist of 17 exons spanning at least 40 kb in the genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380050007
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    Paper (German National Licenses)
    Fulltext
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