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  • 1
    ISSN: 1569-8041
    Keywords: Interleukin (IL)-10 mRNA ; IL-10 receptor mRNA ; non-small-cell lung cancer (NSCLC) ; prognosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background:The type 2 cytokine interleukin (IL)-10 has beenreported to inhibit the antitumour activity of the regional immunity againstvarious neoplasms. Certain lung cancers produce IL-10, but the clinicalsignificance of IL-10 expression is not well understood. Patients and methods:We examined IL-10 and IL-10 receptor(IL-10R) mRNA expression in 82 non-small-cell lung cancers (NSCLC) by reversetranscription-polymerase chain reaction (RT–PCR) assay.Immunohistochemistry (IHC) and enzyme immunoassay (EIA) were applied toevaluate the cellular localisation and the serum levels of IL-10. Results:RT–PCR assay revealed IL-10 mRNA expression in 68(83%) of 82 NSCLC surgical specimens (40 of 50 adenocarcinomas, 22 of26 squamous cell carcinomas, 5 of 5 large-cell carcinomas, 1 of 1adenosquamous-cell carcinoma). RT–PCR assay also revealed IL-10R mRNAexpression in 79 cases of NSCLC (96.1%). IL-10 expression was confirmedwithin tumour cells by IHC. EIA showed no significant serum IL-10 elevationin the 12 NSCLC positive for IL-10 mRNA expression (0–2.99 pg/ml). TheNSCLC patients with IL-10 production showed significantly poorer prognosisthan those without IL-10 production (P 〈 0.05, Kaplan–Meier,log-rank test). Conclusions:These results suggested that the cytoplasmic IL-10correlated to clinical prognosis, and that IL-10 expression is a prognosticfactor for NSCLC.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of ethology 18 (2000), S. 127-131 
    ISSN: 1439-5444
    Keywords: Key words Adaptive significance ; Winter pair bond ; Northen pintail ; Cost and benefit ; Feeding and social behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To test the female-advantage hypothesis that has been proposed to explain the adaptive significance of winter pair bonds in ducks, we examined the feeding and social behaviors of the northern pintail, Anas acuta. The female-advantage hypothesis assumes that male attendance offers paired females the benefits of increased social status and access to food, as well as less harassment from conspecifics, allowing them to spend more time feeding. Paired females dominated unpaired females, but neither time budgets of feeding nor frequency of feeding was significantly different between unpaired and paired females. The female-advantage hypothesis predicts that paired males spend less time feeding because they must closely guard their partners from harassment by male conspecifics. Paired males defended their mates by chasing and pecking the unpaired males. However, both time budgets of feeding and frequency of feeding were significantly higher in paired males than in unpaired males. Unpaired males frequently approached females while swimming. They performed courtship displays, mostly toward unpaired females. Paired males spent more time feeding by saving time and energy in courtship. We consider that the advantage of winter pairing for males comes from having a mate plus having an increase in feeding frequency.
    Type of Medium: Electronic Resource
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