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  • 2000-2004  (2)
  • Cryptosporidium  (1)
  • DNA conformation  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Heteroduplex mobility assay (HMA) pre-screening: An improved strategy for the rapid identification of inserts selected from phage-displayed peptide libraries (2000)
    Springer
    Molecular diversity 5 (2000), S. 7-12 
    Details
    ISSN: 1573-501X
    Keywords: DNA conformation ; heteroduplex mobility assay (HMA) ; mismatch ; mutation detection ; phage display
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Phage-displayed peptide libraries represent an efficient toolto isolate peptides that bind a given target molecule. Afterseveral selection rounds, generally a large pool of targetbinding phages is obtained. Conventional analysis of theselected phage population involves extensive sequencing of many clones, mostof which can be identical. We have adapted the HeteroduplexMobility Assay (HMA) for pre-screening of phage inserts thatwere amplified by direct colony PCR of ELISA-positive clones.This strategy allowed for the rapid and reproducible assignment ofinsert sequences to different `heteroduplex migration groups'.Sequence analysis of only one representative of each HMAmigration group then completes the characterisation of thebinding phage population. In our model experiments,only 16% of HMA pre-screenedclones required further sequence analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1011318710547
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Serological evidence of Cryptosporidium infections in southern Europe (2000)
    Springer
    European journal of epidemiology 16 (2000), S. 385-390 
    Details
    ISSN: 1573-7284
    Keywords: Cryptosporidium ; Endemic infections ; Serology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Although cryptosporidiosis outbreaks have been frequently reported in the United States, Canada and the United Kingdom, few outbreaks have been reported on the European continent. The reasons for this are unclear. To ascertain whether a European population has been previously exposed to Cryptosporidium, we conducted a survey of 100 resident blood donors in a northern Italian city for IgG serological response to two oocyst antigen groups. A serological response to the 15/17-kDa antigen group was detected in 83% of blood donors and response to the 27-kDa antigen group in 62%. Donors who traveled outside of Italy during the prior 12 months were less likely to have had a response to the 15/17-kDa antigen group (p 〈 0.04) and to have a less intense response (p 〈 0.05). Older age was predictive of a more intense response to each antigen group (p 〈 0.01). The fraction of Italian blood donors with a serological response to either antigen group was higher than in four United States blood donor populations, with differences more pronounced for response to the 15/17-kDa antigen group (p 〈 0.01). A lower fraction of Italian donors had a serological response to either antigen group than persons tested at the time of a cryptosporidiosis outbreak in the United States or blood donors tested six months after that outbreak (p 〈 0.05). Since the presence of serological responses to these antigen groups predicts a reduced risk of cryptosporidiosis, the high prevalence of serological responses in these Italian blood donors may explain the infrequent occurrences of clinically detectable cryptosporidiosis in this city.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007654226975
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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