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  • 2000-2004  (3)
  • Microbial biomass carbon  (1)
  • Mobility  (1)
  • pluripotency  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Seasonal changes of microbial biomass carbon related to climatic factors in soils from karst areas of southwest China (2000)
    Springer
    Biology and fertility of soils 30 (2000), S. 294-297 
    Details
    ISSN: 1432-0789
    Keywords: Key words Seasonal changes ; Microbial biomass carbon ; Climatic factors ; Karst
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The seasonal responses of soil microbial biomass C to changes in atmospheric temperature, soil moisture and soluble organic C were studied in soils from the karst areas of southwest China. These soils are relatively weathered, leached and impoverished, and have a low input of plant residues. Over 1 year, an inverse relationship between soil microbial biomass C and atmospheric temperature was found. The highest microbial biomass C occurred in winter and the lowest in summer, and ranged from 231–723 μg g–1 dry soil. Although there was no obvious relationship between microbial biomass C and soil moisture, a negative correlation existed between microbial biomass C and soluble organic C. In the ecosystem studied, the marked changes in soil microbial biomass C at above 20  °C were ascribed to fluctuations of soil moisture, which were controlled by climatic factors and geomorphic conditions. The patterns of soluble organic C turnover were similar to those of soluble carbohydrate C, both of which were controlled by soil drying-rewetting cycles. It was concluded that the lowest amounts of soil microbial biomass C, measured in the summer, resulted in increases in soluble organic C due to higher turnover rates of the former at warmer air temperatures. Thus, there was a marked seasonal change in soil microbial biomass C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050006
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Mobility of some potentially toxic trace elements in the coal of Guizhou, China (2000)
    Springer
    Environmental geology 39 (2000), S. 372-377 
    Details
    ISSN: 1432-0495
    Keywords: Key words Coal Potentially toxic trace elements ; Speciation ; Mobility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract  The mobility of 10 potentially toxic trace elements (PTTE), As, Cd, Cr, Cu, Hg, Ni, Pb, Se, Tl, and Zn from 32 coals of the Longtan Group formed in Permian Period in Guizhou Province, China was investigated using sequential extraction procedures. The results demonstrate that PTTEs such as Hg, As, Se, Cd, Cu, and Pb have the highest mobility at surface conditions, and the average extractable fractions of them are 86%, 95%, 79%, 76%, 69%, and 69% of the total amount in coal, respectively. The elements in coal with the lowest leachability include Tl, Cr, and Ni, and the average extractable fractions of them are 30%, 20%, and 29% of the total amount in coal respectively. Zinc has an intermediate behavior, and the average leachable fraction of it accounts for 46% of the total amount in coal. The results demonstrate that mobility of PTTE in coal depends on the speciation of these elements. The elements associated with sulfates, carbonates, sulfides and some organic matter in coal show the highest extraction rates during the weathering process, while elements with silicate affinities are inert at surface conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002540050016
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Embryonic stem cells in fish: current status and perspectives (2000)
    Springer
    Fish physiology and biochemistry 22 (2000), S. 165-170 
    Details
    ISSN: 1573-5168
    Keywords: chimera ; ES ; gene targeting ; medaka ; pluripotency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Totipotent embryonic stem (ES) cells represent a bridge that links in vitro and in vivo manipulations of animal genomes and have enormous potential for genetic engineering of livestock. We have recently established feeder cell-free conditions for culturing cells of midblastula embryos (MBE) of the medaka (Oryzias latipes) and obtained several stable cell lines that show all features of mouse ES cells in vitro. One of these lines, MES1, has been demonstrated to retain a diploid karyotype and can be induced to differentiate into various cell types in vitro. Upon microinjection into albino host blastulae, MES1 cells are able to form pigmented chimeras. Genotype-specific PCR analysis revealed that 90% of host blastulae transplanted with MES1 cells developed into chimeric fry. This high frequency was not compromised by cryostorage or DNA transfection of the donor cells. Transplantation of genetically labelled MES1 cells revealed a wide contribution to numerous organs derived from all three germ layers and differentiation into various types of functional cells. These ES properties of MES1 line was not abolished by stable gene transfer and long-term selection. Thus MES1 cells may represent a first promising cellular vehicle for the production of genetically modified fish. The genetic background has been found to have a profound effect on the efficacy of ES cell derivation and of chimera formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007871121784
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    Paper (German National Licenses)
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