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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Environmental geology 39 (2000), S. 372-377 
    ISSN: 1432-0495
    Keywords: Key words Coal Potentially toxic trace elements ; Speciation ; Mobility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract  The mobility of 10 potentially toxic trace elements (PTTE), As, Cd, Cr, Cu, Hg, Ni, Pb, Se, Tl, and Zn from 32 coals of the Longtan Group formed in Permian Period in Guizhou Province, China was investigated using sequential extraction procedures. The results demonstrate that PTTEs such as Hg, As, Se, Cd, Cu, and Pb have the highest mobility at surface conditions, and the average extractable fractions of them are 86%, 95%, 79%, 76%, 69%, and 69% of the total amount in coal, respectively. The elements in coal with the lowest leachability include Tl, Cr, and Ni, and the average extractable fractions of them are 30%, 20%, and 29% of the total amount in coal respectively. Zinc has an intermediate behavior, and the average leachable fraction of it accounts for 46% of the total amount in coal. The results demonstrate that mobility of PTTE in coal depends on the speciation of these elements. The elements associated with sulfates, carbonates, sulfides and some organic matter in coal show the highest extraction rates during the weathering process, while elements with silicate affinities are inert at surface conditions.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Fish physiology and biochemistry 22 (2000), S. 165-170 
    ISSN: 1573-5168
    Keywords: chimera ; ES ; gene targeting ; medaka ; pluripotency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Totipotent embryonic stem (ES) cells represent a bridge that links in vitro and in vivo manipulations of animal genomes and have enormous potential for genetic engineering of livestock. We have recently established feeder cell-free conditions for culturing cells of midblastula embryos (MBE) of the medaka (Oryzias latipes) and obtained several stable cell lines that show all features of mouse ES cells in vitro. One of these lines, MES1, has been demonstrated to retain a diploid karyotype and can be induced to differentiate into various cell types in vitro. Upon microinjection into albino host blastulae, MES1 cells are able to form pigmented chimeras. Genotype-specific PCR analysis revealed that 90% of host blastulae transplanted with MES1 cells developed into chimeric fry. This high frequency was not compromised by cryostorage or DNA transfection of the donor cells. Transplantation of genetically labelled MES1 cells revealed a wide contribution to numerous organs derived from all three germ layers and differentiation into various types of functional cells. These ES properties of MES1 line was not abolished by stable gene transfer and long-term selection. Thus MES1 cells may represent a first promising cellular vehicle for the production of genetically modified fish. The genetic background has been found to have a profound effect on the efficacy of ES cell derivation and of chimera formation.
    Type of Medium: Electronic Resource
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