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  • 1
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Allergy 56 (2001), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In addition to the well-known actions of the humoral renin–angiotensin system, all components of this system are present in many tissues, including the brain, and may play a major role in brain development and differentiation. We investigated the possible effects of angiotensin II on the generation of dopaminergic phenotype neurons from proliferating neurospheres of mesencephalic precursors. We observed immunoreactivity for both angiotensin type 1 and type 2 (AT1 and AT2) receptors in the cell aggregates. Double immunolabeling studies revealed that both receptor types are located in neurons and astrocytes. Interestingly, neurons with a dopaminergic phenotype (i.e. tyrosine hydroxylase activity) showed double labeling for AT1 and AT2 receptors although the labeling for AT2 was more intense. Treatment of the neurospheres with angiotensin II (100 nm) during the differentiation period induced a marked increase (about 400%) in the generation of dopaminergic neurons. This was not affected by treatment with the AT1 antagonist ZD 7155 but was blocked by treatment with the AT2 antagonist PD 123319. This suggests that AT2 receptors mediate the stimulatory effect of angiotensin II on the generation of dopaminergic neurons. Apoptotic cell death studies and bromodeoxyuridine immunohistochemistry indicated that the increase in generation of dopaminergic neurons is not due to increased survival or proliferation of dopaminergic cells during treatment with angiotensin and suggested that angiotensin induces increased differentiation of mesencephalic precursors towards the dopaminergic phenotype. Manipulation of the renin–angiotensin system may be useful for increasing production of dopaminergic neurons for transplantation in Parkinson's disease.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2072
    Keywords: Key words Cyamemazine ; Anxiety ; Serotonin ; 5-HT3-receptor ; 5-HT2C-receptor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Rationale: Cyamemazine is a neuroleptic compound which possesses anxiolytic properties in humans. On the other hand, 5-HT3- and 5-HT2C-receptors have been implicated in anxiety disorders and a previous binding study has shown that cyamemazine possesses high affinity for both serotonin receptor types. Objective: The present study was undertaken to establish whether cyamemazine antagonizes 5-HT3- and/or 5-HT2C-mediated responses, and whether it compares with reference compounds. Methods: Cyamemazine was tested for its ability to antagonize: (i) 5-HT3-dependent contraction of the isolated guinea-pig ileum and bradycardic responses in the rat and (ii) 5-HT2C-dependent phospholipase C (PLC) stimulation in rat brain membranes. Results: In isolated guinea-pig ileum, cyamemazine potently and competitively antagonized 5-HT-dependent contractions (pA2=7.52±0.08; n=5). In this test, cyamemazine was 5–7 times more potent (pIC50=6.75±0.13) than tropisetron (pIC50=6.02±0.04). In rats, cyamemazine i.v. antagonized 5-HT-dependent bradycardic responses with ID50%=3.2±1.5 mg/kg (n=4). Finally, in rat brain membranes cyamemazine antagonized 5-HT2C-dependent PLC stimulation with Ki=424 nM (mianserin exhibits a Ki=113 nM). Conclusions: Cyamemazine behaves as an antagonist at both 5-HT3- and 5-HT2C-receptors, which compares well with reference compounds. These 5-HT3- and 5-HT2C-antagonistic actions of cyamemazine can be involved, at least in part, in its beneficial therapeutic actions in anxiety disorders.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1433-0350
    Keywords: Key words Occipital seizures ; Stormy onset ; Benign epilepsy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We studied six children with ages ranging from 4 to 10 years who were affected by childhood epilepsy with occipital paroxysms and presented after a stormy onset with prolonged loss of consciousness for 6–14 h. In all these patients, seizures were preceded by visual symptoms in the form of colored circular disks. A CT scan was performed immediately after the onset of symptoms and was normal in all patients. Routine laboratory and cerebrospinal fluid examinations were normal in all patients. The interictal EEG was characterized by continuous or subcontinuous occipital spike wave discharges, which disappeared after the patients’ eyes opened. We carried out a 7-year follow-up of all these patients. Only two patients were treated with antiepileptic drugs. The therapy (phenobarbital, clobazam) in the two patients did not induce changes in the EEG pattern. The first did not suffer any further seizures. The second patient had two more seizures (at 8 and 18 months from the onset) with phosphenes, confusional state, and involuntary movements followed by loss of consciousness. Among the other four patients, who did not receive any treatment, only one had any other seizures. The stormy onset of the syndrome described in our six patients emphasizes the extreme variability in the presentation of this type of childhood epilepsy. Our follow-up confirms the good prognosis of this epilepsy even when it has a stormy onset.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 100 (2000), S. 361-367 
    ISSN: 1432-2242
    Keywords: Key words Citrus sinensis ; CMA banding ; FISH ; rRNA genes ; Heterochromatin ; Heterozygosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Citrus sinensis chromosomes, although small in size, present a remarkable differentiation of bands with the fluorochromes CMA and DAPI. These bands suggest that some heteromorphisms are fixed in this species. To investigate the extension of these heteromorphisms, ten cultivars of C. sinenesis were analysed with CMA/DAPI staining and, in some of them, the 18S–5.8S–25S rRNA and 5S rRNA genes were located by in situ hybridization. CMA/DAPI staining showed exactly the same CMA+/DAPI− banding pattern for all cultivars. In situ hybridization revealed three 18S–5.8S–25S rRNA gene sites, two proximally located on two similar chromosomes and one terminally located on a third non-related chromosome. Two 5S rRNA gene sites were observed in this species, with one located proximal to the telomeric 18S–5.8S–25S rDNA site. Both cytological approaches revealed an invariable, heterozygotic karyotype among sweet orange cultivars. Based on these data, the putative hybrid origin of the species is discussed.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of dynamical and control systems 6 (2000), S. 265-309 
    ISSN: 1573-8698
    Keywords: singular L-Q optimal control problems ; relaxed controls ; existence of minimizers ; characterization of minimizers ; approximation of relaxed controls
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract It is well known that singular problems may fail to have optimal solution in the class of “ordinary” (say, square-integrable) controls, even in the cases where the cost is bounded from below. In this paper, we suggest a method for overcoming this difficulty by defining an order r of singularity of the problem and extending both the input-trajectory map and the cost functional to an adequate subspace of the Sobolev space H_r-r. We show that the extended problem has a minimum if and only if the infimum of the original problem is finite. The extended problem can be transformed in a “natural” way into a regular L-Q problem with strictly smaller controllable space and (possibly) smaller control space. We use this transformation to describe the structure of relaxed optimal controls and the corresponding relaxed trajectories. We provide a method for computing optimal relaxed solutions from the solution of an adequate Riccati differential equation. We also show how the relaxed minimizers can be approximated by square-integrable functions.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-157X
    Keywords: Apennines-Central Italy ; earthquake chemistry-isotope chemistry ; fluids behaviour-seismicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract After the earthquakes of September 26, 1997, that hit the Umbria-Marcheboundary (Apennine, Central Italy), with a maximum 6.0 Mw, aprogram of geochemical surveying together with a collection ofhydrogeological changes episodes was extended throughout theepicentre-area, taking the yearly period of the seismic sequence as a whole.After a first areal screening, the Bagni di Triponzo thermal spring wasselected for a discrete temporal monitoring (weekly and monthly basis),being the unique thermal spring throughout the epicentre area. This sitedeserves peculiar interest in deepening the knowledge about deep fluidscirculation changing during seismicity.Laboratory and on-field analyses included major, minor and trace elementsas well as dissolved gases (He, Ar, CH4, CO2, H2S,222Rn, NH4, As, Li, Fe, B, etc...) and selected isotopic ratios(C, H, O, He, Sr, Cl), meaningful from tectonic point of view.The chemistry and isotopic chemistry of the spring were fully outlined anddiscussed, pointing out the main process involving the thermal aquifer: thewater-rock interaction inside the Evaporite Triassic Basement (ETB),possibly involving also the Paleozoic Crystalline Basement. On theother hand, sudden and apparent geochemical and hydrogeologicalvariations during the seismic sequence ruled out an evolution in thewater-rock interaction processes. They occurred both at depth, i.e.,induced by fluid remobilization within the crust explained by the Coseismic Strain Model and by the Fault Valve Activity Model, and in the shallow part of the reservoir (i.e., meteoric watercontamination). A statistical multivariable analysis (Factor Analysis) wasaccomplished to better constrain the correlation between the paroxysmalphases of the seismic sequence and the observed trends and spike-likeanomalies. The groundwater variations was inferred to occur mainly insidethe ETB, from depth (1–2 km) up to surface, particularly in associationof the Sellano earthquake (14/10/1997) and of the seismic re-activationof the sequence at the end of March 1998 (Gualdo Tadino-Rigali andVerchiano areas). The lack of deeper input from below the ETB (slightsignature of PCB), as the lack of He mantle signature, during the seismicperiod as a whole, accounted for seismogenic fault segments rooted onlyin the crust. The results also provide useful information about theearthquake-related response mechanisms occurring at this site, thatrepresent the basic task for planning and managing the impendinghydro-geochemical network aimed at defining the relationships betweenseismic cycle, fluids and reliable earthquake forerunners.
    Type of Medium: Electronic Resource
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