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  • 2000-2004  (8)
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  • 1
    Digitale Medien
    Digitale Medien
    Education, cost of living and regional wage inequality in Brazil (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Papers in regional science 81 (2002), S. 0 
    Details
    ISSN: 1435-5957
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Geographie , Wirtschaftswissenschaften
    Notizen: Abstract The objective of this article is to analyze wage inequality among the 10 largest metropolitan regions in Brazil in the 1990s. We assess the extent to which worker characteristics (education, age, gender, race, position in the family) and job characteristics (occupational position, sector, experience) can explain wage inequality. The analysis is made both with regional-nominal and with regional-real wage data. In the second case regional price indexes are used to control for differences in cost of living among regions. Wage differentials in Brazil were slightly lowered when control variables were introduced, but the leftover inequality remained high. The results indicate that cost of living levels do have a role in explaining wage inequality in Brazil, but even after controlling for this factor, the remaining regional differentials are still important.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1435-5597.2002.tb01228.x
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  • 2
    Digitale Medien
    Digitale Medien
    Dpo4 is hindered in extending a G·T mismatch by a reverse wobble (2004)
    [s.l.] : Nature Publishing Group
    Nature structural & molecular biology 11 (2004), S. 457-462 
    Details
    ISSN: 1545-9985
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Medizin
    Notizen: [Auszug] The ability or inability of a DNA polymerase to extend a mispair directly affects the establishment of genomic mutations. We report here kinetic analyses of the ability of Dpo4, a Y-family polymerase from Sulfolobus solfataricus, to extend from all mispairs opposite a template G or T. Dpo4 is ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/nsmb755
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  • 3
    Digitale Medien
    Digitale Medien
    Identification of tdh-positive Vibrio parahaemolyticus from an outbreak associated with raw oyster consumption in Spain (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 226 (2003), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Between August and September 1999, a total of 64 cases of illness were identified in three episodes of acute gastroenteritis associated with the consumption of live oysters from a typical outdoor street market in Galicia (northwest Spain). Nine case patients were hospitalized and analysis of their stool samples revealed the presence of Vibrio parahaemolyticus. The strains isolated from two stool samples were studied for antibiotic susceptibility, biochemical characteristics and presence of virulence factors. Both isolates were Kanagawa phenomenon positive and produced thermostable direct hemolysin, which is related to pathogenicity in humans. These results show the presence of pathogenic V. parahaemolyticus in mollusks harvested in Europe and reveal the risk of illness associated with their consumption, suggesting the revision of V. parahaemolyticus risk assessment associated with consumption of raw live shellfish.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/S0378-1097(03)00604-9
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  • 4
    Digitale Medien
    Digitale Medien
    Swm1p, a subunit of the APC/cyclosome, is required to maintain cell wall integrity during growth at high temperature in Saccharomyces cerevisiae (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 234 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Swm1p, a subunit of the APC cyclosome, was originally identified for its role in the later stages of the sporulation process and is required for spore wall assembly. In addition, this protein is required to maintain cell wall integrity in vegetative cells during growth at high temperature. Electron microscopy analyses of mutant cells grown at the restrictive temperature in the absence of osmotic support show that the cell wall is clearly abnormal, with large number of discontinuities that may be responsible for the observed lysis. The mutant cells show a 7-fold reduction in glucan synthase activity during growth at 38 °C and a 3.5-fold increase in the chitin content of the cell wall. The chitin is deposited in a delocalized manner all over the cell wall, where it accumulates in patches in abnormal regions. The excess chitin is mainly synthesized by the action of chitin synthase III (Chs3p), since it disappears in the swm1 chs3 double-mutant.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.2004.tb09556.x
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  • 5
    Digitale Medien
    Digitale Medien
    Contribution of the periosteum to bone formation in guided bone regeneration (2000)
    Copenhagen : Munksgaard International Publishers
    Clinical oral implants research 11 (2000), S. 0 
    Details
    ISSN: 1600-0501
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: The periosteum has been referred to as a protective barrier in the regeneration of bone defects. The objective of this study was to determine the contribution of periosteum as a natural barrier to bone formation in guided bone regeneration. Mucoperiosteal flaps were elevated bilaterally on the buccal aspect of the mandibular angle in 5 cynomolgus monkeys. Bleeding was induced by perforating the cortical bone. A hemispherical titanium mesh was fixed over the areas thus creating a void 5 mm in height between the mesh and the bone surface. On one side the mesh was covered with an ePTFE membrane (test side). The contralateral side did not receive further treatment (control side). After 4 month healing, histomorphometric analyses were used to determine the percentage of new bone in the void underneath the mesh, and the ratio between mineralized tissue and marrow spaces in new and old bone. The mean percentage of new bone tissue was 77.2±7.5% for the test sides and 68.6±8.4% for the control sides (P=0.018, t-test). This new bone contained 80.0±3.6% mineralized tissue in the test group and 82.5±5.0% in the control group (P〉0.05, t-test). In both groups the newly formed bone exhibited significantly less mineralized tissue than the old bone (P〈0.05, t-test). It is concluded from this study that new bone formation was enhanced by the additional use of an ePTFE membrane under a periosteum-lined mucoperiosteal flap when space maintenance was excluded as a critical factor.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1034/j.1600-0501.2000.011006546.x
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  • 6
    Digitale Medien
    Digitale Medien
    Characterization of an amylase produced by a Trichoderma harzianum isolate with antagonistic activity against Crinipellis perniciosa, the causal agent of witches’ broom of cocoa (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 188 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: An isolate of Trichoderma harzianum showing antagonistic activity against Crinipellis perniciosa, the causal agent of the witches’ broom disease of cocoa, produces substantial amounts of hydrolytic enzymes. An amylase purified from isolate 1051 had a molecular mass of about 68.7 kDa. Maximal activity against soluble starch was determined at pH 4.0 and 60°C. The Km and Vmax values were 3.5 mg ml−1 and 1.67 mg min−1 of reducing sugar. The end products were mostly malto-oligosaccharides. The enzyme also hydrolyzed glycogen, amylopectin, maltotriose, and maltotetraose, but not pullulan or cellobiose. Maltose was only barely hydrolyzed. The purified amylase exerted a discrete hydrolytic effect on the C. perniciosa cell wall in vitro as observed by scanning electron microscopic analysis. While Fe3+, Al3+, Zn2+, and Cu2+ were effective in inhibiting the purified amylase, Mn2+ considerably enhanced the activity. Ca2+, Mg2+, and Co2+ showed no substantial effect on enzyme activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09189.x
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  • 7
    Digitale Medien
    Digitale Medien
    Syringe Pressure Irrigation of Subdermic Tissue after Appendectomy to Decrease the Incidence of Postoperative Wound Infection (2000)
    Springer
    World journal of surgery 24 (2000), S. 38-42 
    Details
    ISSN: 1432-2323
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract. To evaluate syringe pressure irrigation of the surgical wound to decrease its infection after appendectomy, we designed a randomized control trial at the Emergency Department of Mexico City General Hospital, including 350 patients with acute abdomen suggestive of appendicitis, without any other infection clinically evident. The trial was randomized into 2 groups. Group I patients received prophylactic systemic antibiotics before surgery. Group II patients received the same prophylactic systemic antibiotics plus syringe pressure irrigation of the surgical wound with 300 ml of saline solution using a 20-ml syringe with 19-gauge intravenous (IV) catheter to measure the incidence of postoperative wound infection. In our results, 283 patients had appendicitis. Of these, 188 were uncomplicated (66.4%) and 95 (33.6%) were complicated. Of the complicated cases, 40 were assigned to group I, and of these, 29 (72.5%) developed wound infection. In group II there were 55 patients and only 9 (16.3%) developed wound infection after syringe pressure irrigation [p= 0.000001; 95% confidence interval (CI) = 0.02–0.22]. We conclude that syringe pressure irrigation of the surgical wound after appendectomy contributes significantly to decrease the incidence of postoperative wound infection in complicated cases. It is a cheap, safe, and accessible method in any surgical room.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002689910008
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  • 8
    Digitale Medien
    Digitale Medien
    A Trichoderma harzianum chitinase destroys the cell wall of the phytopathogen Crinipellis perniciosa, the causal agent of witches' broom disease of cocoa (2000)
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 383-386 
    Details
    ISSN: 1573-0972
    Schlagwort(e): Biological control ; chitinase ; Crinipellis perniciosa ; micoparasitism ; Trichoderma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract A Trichoderma harzianum isolate (1051), which was able to antagonize in field the phytopathogen Crinipellis perniciosa, the causal agent of witches' broom disease of cocoa, produces several hydrolytic enzymes. A chitinase, with molecular mass of about 37 kDA, which was secreted by the Trichoderma in the culture medium containing chitin, was partially purified by gel filtration followed by hydrophobic chromatography. The optimal pH and temperature for chitin hydrolysis by the partially purified enzyme were 4.0 and 37 °C, respectively. Chitobiose, laminarin, cellulosic substrates including aryl-glucosides, xylan, starch and β-galactomannan were not hydrolysed by the enzyme. Remarkably, the partially purified enzyme drastically affected the cell wall of the phytopathogen C. perniciosa in vitro.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008964324425
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