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Identification of intermediates of in vivo trichloroethylene oxidation by the membrane-associated methane monooxygenase (2000)

Lontoh, Sonny ; Zahn, James A. ; DiSpirito, Alan A. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 186 (2000), S. 0

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ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: The rate and products of trichloroethylene (TCE) oxidation by Methylomicrobium album BG8 expressing membrane-associated methane monooxygenase (pMMO) were determined using 14C radiotracer techniques. [14C]TCE was degraded at a rate of 1.24 nmol (min mg protein)−1 with the initial production of glyoxylate and then formate. Radiolabeled CO2 was also found after incubating M. album BG8 for 5 h with [14C]TCE. Experiments with purified pMMO from Methylococcus capsulatus Bath showed that TCE could be mineralized to CO2 by pMMO. Oxygen uptake studies verified that M. album BG8 could oxidize glyoxylate and that pMMO was responsible for the oxidation based on acetylene inactivation studies. Here we propose a pathway of TCE oxidation by pMMO-expressing cells in which TCE is first converted to TCE-epoxide. The epoxide then spontaneously undergoes HCl elimination to form glyoxylate which can be further oxidized by pMMO to formate and CO2.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09090.x

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