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  • 2000-2004  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Experimental dermatology 9 (2000), S. 0 
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Cholesterol synthesis is essential for homeostasis of the epidermis, being required for both cell division and differentiation, as well as maintenance of the epidermal permeability barrier. Cholesterol synthesis in keratinocytes has been demonstrated to be regulated by sterol levels and the barrier function of the stratum corneum. Cholesterol synthesis in the epidermis is correlated with changes in mRNA levels for key enzymes, such as HMG-CoA synthase and HMG-CoA reductase, which have been previously demonstrated to be coordinately regulated by the sterol regulatory element binding proteins (SREBPs). In this study we demonstrate that a functional sterol regulatory element is required for sterol regulation of HMG-CoA synthase in keratinocytes. We also investigate the regulation of cholesterol synthesis by fatty acids, which are another important constituent of the stratum corneum lipids. Palmitic and oleic acid inhibit 14C-labelled acetate incorporation into sterols in a similar manner to sterols. However, unlike sterols, 50 μM oleic acid increase the steady state mRNA levels of HMG-CoA synthase and the activity of the HMG-CoA synthase promoter. The addition of 50 μM oleic acid to 25-hydroxycholesterol results in an enhancement of the inhibitory effect of the sterol on promoter activity. The inhibition of acetate incorporation into sterols in human keratinocytes by 50 μM palmitic and 50 μM oleic acid is not due to regulation of HMG-CoA synthase at the level of transcription.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    Kybernetes 33 (2004), S. 962-972 
    ISSN: 0368-492X
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Computer Science
    Notes: Ecosystem behavior is complex and may be controlled by many factors that change in space and time. Consequently, when exploring system functions such as ecosystem "health", scientists often measure dozens of variables and attempt to model the behavior of interest using combinations of variables and their potential interactions. This methodology, using parametric or nonparametric models, is often flawed because ecosystems are controlled by events, not variables, and events are comprised of (often tiny) pieces of variable combinations (states and substates). Most events are controlled by relatively few variables (=4) that may be modulated by several others, thereby creating event distributions rather than point estimates. These event distributions may be thought of as comprising a set of fuzzy rules that could be used to drive simulation models. The problem with traditional approaches to modeling is that predictor variables are dealt with in total, except for interactions, which themselves must be static. In reality, the "low" piece of one variable may influence a particular event differently than another, depending on how pieces of other variables are shaping the event, as demonstrated by the k-systems state model of algal productivity. A swamp restoration example is used to demonstrate the changing faces of predictor variables with respect to influence on the system function, depending on particular states. The k-systems analysis can be useful in finding potent events, even when region size is very small. However, small region sizes are the result of using many variables and/or many states and substates, which creates a high probability of extracting falsely-potent events by chance alone. Furthermore, current methods of granulating predictor variables are inappropriate because the information in the predictor variables rather than that of the system function is used to form clusters. What is needed is an iterative algorithm that granulates the predictor variables based on the information in the system function. In most ecological scenarios, few predictor variables could be granulated to two or three categories with little loss of predictive potential.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Macmillian Magazines Ltd.
    Nature 412 (2001), S. 202-206 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Genetic studies have identified over a dozen genes that function in programmed cell death (apoptosis) in the nematode Caenorhabditis elegans. Although the ultimate effects on cell survival or engulfment of mutations in each cell death gene have been extensively described, much less is ...
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1866
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract In the Mazowe area some 40 km NW of Harare in Zimbabwe, gold mineralization is hosted in a variety of lithologies of the Archean Harare-Bindura-Shamva greenstone belt, in structures related to the late Archean regional D2/3 event. Conspicuous mineralzogical differences exist between the mines; the mainly granodiorite-hosted workings at Mazowe mine are on pyrite-rich reefs, mines of the Bernheim group have metabasalt host rocks and are characterized by arsenopyrite-rich ores, and Stori's Golden Shaft and Alice mine, both in metabasalts, work sulfide-poor quartz veins. In contrast to the mineralogical diversity, near-identical fluid inventories were found at the different mines. Both H2O-CO2-CH4 fluids of low salinity, and highly saline fluids are present and are regarded to indicate fluid mixing during the formation of the deposits. Notably, these fluid compositions in the Mazowe gold field markedly contrast to ore fluids “typical” of Archean mesothermal gold deposits on other cratons. Stable isotope compositions of quartz from the various deposits (δ18O=10.8 to 13.2‰ SMOW), calcite (δ18O=9.5 to 11.9‰ SMOW and δ13C=−3.2 to −8.0‰ PDB), inclusion water (δD=−28 to −40‰ SMOW) and sulfides (δ34S=1.3 to 3.2‰ CDT) are uniform within the range typical for Archean lode gold deposits worldwide. The fluid and stable isotope compositions support the statement that the mineralization in the Mazowe gold field formed from relatively reduced fluids with a “metamorphic” signature during a single event of gold mineralization. Microthermometric data further indicate that the deposits formed in the PT range of 1.65–2.3 kbar and 250–380 °C. Ages obtained by using the Sm/Nd and Rb/Sr isotope systems on scheelites are 2604 ± 84 Ma for the mineralization at Stori's Golden Shaft mine, and 2.40 ± 0.20 Ga for Mazowe mine. The Archean age at Stori's is regarded as close to the true age of gold mineralization in the area, whereas the Proterozoic age at Mazowe mine probably reflects later resetting.
    Type of Medium: Electronic Resource
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