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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Thirty-six wheat-Agropyron intermedium (host) Beauv. [Syn. Trichopyrum intermedium (host) A. Love, Elytrigia intermedia (host) Nevski, Thinopyrum intermedium (host) Barkworth and Dewey] 7A/7Ai-1 recombinant chromosomes were characterized using DNA markers. Analysis of recombinant chromosomes using 15 restriction fragment length polymorphism probes identified the homoeologous crossover products that had varying length of A. intermedium chromatin introgressed onto chromosome 7A of common wheat. The linear order of the probe loci was established along the lengths of the chromosomes. The short arm recombinants that had A. intermedium chromatin distal to the locus Xpsr108 and proximal to the locus Xpsr119 were resistant to wheat stem rust, indicating that the rust resistance gene (Sr44) was located on the distal part of chromosome arm 4Ai-1s. The barley yellow dwarf virus (BYDV) resistance gene reported to be present on the long arm of chromosome 7Ai-1 was found to be ineffective against the BYDV serotype used in the present study.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 100 (2000), S. 1137-1143 
    ISSN: 1432-2242
    Keywords: Key words Wheat ; Triticum speltoides ; Marker-assisted selection ; Leaf rust ; Resistance gene ; PCR markers ; Sucrose synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The leaf rust resistance gene Lr47 confers resistance to a wide spectrum of leaf rust strains. This gene was recently transferred from chromosome 7 S of Triticum speltoides to chromosome 7 A of hexaploid wheat Triticum aestivum. To facilitate the transfer of Lr47 to commercial varieties, the completely linked restriction fragment length polymorphism (RFLP) locus Xabc465 was converted into a PCR-based marker. Barley clone ABC465 is orthologous to the type-I wheat sucrose synthase gene and primers were designed for the conserved regions between the two sequences. These conserved primers were used to amplify, clone and sequence different alleles from T. speltoides and T. aestivum. This sequence information was then used to identify the T. speltoides sequence, detect allele-specific mutations, and design specific primers. Cosegregation of the PCR product of these primers and the T. speltoides chromosome segment was confirmed in four backcross-populations. To complement this dominant marker, a cleavage amplified polymorphic sequence (CAPS) was developed for the 7 A allele of Xabc465. This CAPS marker is useful to select homozygous Lr47 plants from F2or backcross-F2 segregating populations, and in combination with the T. speltoides-specific primers is expected to facilitate the deployment of Lr47 in new bread wheat varieties.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Key words Triticum aestivum ; Recombination ; Centromeric region ; Telosomes ; Gametic selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Telosomic stocks have been extensively used to map genes to chromosome arms and to determine gene-to-centromere genetic distances. It has been suggested that if a chromosome arm is present as a telosome, recombination frequencies will be drastically reduced in the centromeric region. However, previous studies have not considered the bias in recombination estimates due to selection against aneuploid gametes produced by failure of pairing at the first meiotic division. Formulas are derived here for adjusting recombination estimates for this bias. Adjusted recombination frequencies between markers located on both sides of the centromeres are analyzed in three different pairs of wheat (Triticum aestivum) isogenic segregating populations involving bibrachial and telocentric chromosomes. Recombination frequencies estimated from crosses involving telocentric chromosomes were not significantly different from recombination frequencies estimated from isogenic crosses involving bibrachial chromosomes. The implications of the present findings for karyotype evolution, and specifically for Robertsonian fissions and fusions, are discussed.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 101 (2000), S. 625-631 
    ISSN: 1432-2242
    Keywords: Keywords Wheat ; Triticum speltoides ; Marker-assisted selection ; Leaf rust ; Resistance gene ; PCR markers ; Sucrose synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The leaf rust resistance gene Lr47 confers resistance to a wide spectrum of leaf rust strains. This gene was recently transferred from chromosome 7S of Triticum speltoides to chromosome 7A of hexaploid wheat Triticum aestivum. To facilitate the transfer of Lr47 to commercial varieties, the completely linked restriction fragment length polymorphism (RFLP) locus Xabc465 was converted into a PCR-based marker. Barley clone ABC465 is orthologous to the type-I wheat sucrose synthase gene and primers were designed for the conserved regions between the two sequences. These conserved primers were used to amplify, clone and sequence different alleles from T. speltoides and T. aestivum. This sequence information was used to identify the T. speltoides sequence, detect allele-specific mutations, and design specific primers. Cosegregation of the PCR product of these primers and the T. speltoides chromosome segment was confirmed in four backcross-populations. To complement this dominant marker, a cleavage amplified polymorphic sequence (CAPS) was developed for the 7A allele of Xabc465. This CAPS marker is useful to select homozygous Lr47 plants from F2 or backcross-F2 segregating populations, and in combination with the T- speltoides specific primers is expected to facilitate the deployment of Lr47 in new bread wheat varieties.
    Type of Medium: Electronic Resource
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