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  • 2000-2004  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Histopathology 37 (2000), S. 0 
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: AimsPromyelocytic leukaemia protein (PML) is an oncoprotein involved in the pathogenesis of acute promyelocytic leukaemia and is localized in distinct PML nuclear bodies. Our previous observation of overexpression of the PML in hormone-sensitive normal tissues and malignant solid tumours, including the thyroid, led to this analysis of the PML expression in various thyroid neoplasms to characterize the importance of the PML in thyroid carcinogenesis.Methods and resultsImmunohistochemistry was performed on paraffin-embedded tissue samples from 106 thyroid neoplasms after antigen retrieval by microwave. Immunoblotting was done with fresh frozen tissues in a few tumours. The PML was strongly expressed in all papillary carcinomas in diffuse or ball-shaped patterns. In the follicular neoplasms, the PML expression was variable, but there was no significant difference between adenomas and carcinomas. In the medullary carcinomas, the PML expression was either not detectable or was lower than in non-neoplastic thyroids. Quantitatively different expression of the PML in various thyroid neoplasms was confirmed by immunoblotting.ConclusionA significant difference of the PML expression according to the type of thyroid neoplasms suggests that the PML is important in papillary thyroid carcinomas, and furthermore, that PML expression may be used in differential diagnosis of thyroid neoplasms.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Microsystem technologies 6 (2000), S. 184-188 
    ISSN: 1432-1858
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Technology
    Notes: Abstract  In this paper, we review work on novel, high aspect processes for microinertial components at the Defence Evaluation and Research Agency (DERA). High aspect components may lead to significant cost-performance improvements in both accelerometers and gyroscopes. We have evaluated 3 low temperature process technologies – silicon on insulator (SOI) HARM, UV electroforming and bulk HARM. Prototype microinertial devices fabricated in these technologies are also presented. The potential of the processes for integration with on-chip CMOS electronics is assessed which may be either as part of a fully integrated MEMS process or as “value-added” post-processing on commercial CMOS wafers. Bonded SOI (BSOI) materials has been specially designed for micromachining applications to give a low stress material that is optimised for a sacrificial release process. Trench isolation is achieved by deep dry etching to the buried dielectric. These trenches may be refilled to allow metallisation to reach isolated components. Structures with aspect ratios of up to 50:1 have been realised using a combination of photolithography, deposition and deep dry etching. CMOS compatibility has been demonstrated. The process is an attractive manufacturing technology. Electroforming of nickel in resist moulds formed using conventional UV photolithography has also been investigated. Some of the early limitations with this technology have been overcome by using a new resist technology, SU8. The process needs to mature further, but remains a promising candidate. Bulk HARM uses deep dry etching of a bulk silicon membrane which is defined using wet etching. Device isolation is difficult and process control complex making this the least attractive of the technologies.
    Type of Medium: Electronic Resource
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