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  • 2000-2004  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The level of random amplified polymorphic DNA (RAPD) and microsatellite variation present in four ecotypes and two varieties of alfalfa (lucerne) from Italian and Egyptian germplasm sources was evaluated. A sample of 100 plants from 10 populations was analysed by means of 41 RAPD markers and 37 simple sequence repeat (SSR) markers. Both molecular approaches revealed a high degree of genetic diversity within each of the cultivated populations and enabled each of the plants considered to be uniquely fingerprinted. The genetic relationships among plants and populations were analysed by computing AMOVA (analysis of molecular variance) and FST analyses. RAPDs were able to separate the Italian populations from the Egyptian variety. SSRs allowed strong separation of the four Italian alfalfa ecotypes. It was concluded that RAPD and microsatellites could be useful and powerful tools for assessing genetic variation and genetic relationships in tetraploid alfalfa.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Four chloroplast simple sequence repeat (cpSSR) loci were identified in alfalfa (lucerne), Medicago sativa L. The occurrence of allelic variation at these loci was evaluated in 100 plants from 10 populations of tetra-ploid alfalfa, belonging to four Italian ecotypes and to an Italian (‘Lodi’) and an Egyptian (‘Iside’) variety. Twenty-four different alleles were identified, four of which were exclusive to the ‘Iside’ variety. The genetic relationship among plants and among populations was analysed by computing an analysis of molecular variance and an unweighted pair group means analysis clustering. This analysis allowed clear separation of the ‘Iside’ variety from Italian germplasm and the recognition of close relationships within the Italian populations. The data presented suggest that cpSSR analysis of tetraploid alfalfa could be used for germplasm polymorphism analysis.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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