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  • 1
    Electronic Resource
    Electronic Resource
    Thermographic visualization of cell death in tobacco and Arabidopsis (2001)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 24 (2001), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Pending cell death was visualized by thermographic imaging in bacterio-opsin transgenic tobacco plants. Cell death in these plants was characterized by a complex lesion phenotype. Isolated cell death lesions were preceded by a colocalized thermal effect, as previously observed at sites infected by tobacco mosaic virus (TMV) (Chaerle et al. 1999Nature Biotechnology 17, 813–816). However, in most cases, a coherent front of higher temperature, trailed by cell death, initiated at the leaf base and expanded over the leaf lamina. In contrast to the homogenous thermal front, cell death was first visible close to the veins, and subsequently appeared as discrete spots on the interveinal tissue, as cell death spread along the veins. Regions with visible cell death had a lower temperature because of water evaporation from damaged cells. In analogy with previous observations on the localized tobacco–TMV interaction (Chaerle et al. 1999), the kinetics of thermographic and continuous gas exchange measurements indicated that stomatal closure preceded tissue collapse. Localized spontaneous cell death could also be presymptomatically visualized in the Arabidopsis lsd2 mutant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2001.00654.x
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  • 2
    Electronic Resource
    Electronic Resource
    Dual action of the active oxygen species during plant stress responses (2000)
    Springer
    Cellular and molecular life sciences 57 (2000), S. 779-795 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Abiotic stress; active oxygen species; biotic stress; catalase; oxidative stress.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Adaptation to environmental changes is crucial for plant growth and survival. However, the molecular and biochemical mechanisms of adaptation are still poorly understood and the signaling pathways involved remain elusive. Active oxygen species (AOS) have been proposed as a central component of plant adaptation to both biotic and abiotic stresses. Under such conditions, AOS may play two very different roles: exacerbating damage or signaling the activation of defense responses. Such a dual function was first described in pathogenesis but has also recently been demonstrated during several abiotic stress responses. To allow for these different roles, cellular levels of AOS must be tightly controlled. The numerous AOS sources and a complex system of oxidant scavengers provide the flexibility necessary for these functions. This review discusses the dual action of AOS during plant stress responses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000180050041
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Sudan-β-d-glucuronides and their use for the histochemical localization of β-glucuronidase activity in transgenic plants (2000)
    Springer
    Plant cell reports 19 (2000), S. 966-970 
    Details
    ISSN: 1432-203X
    Keywords: Key words β-Glucuronidase ; Histochemistry ; Sudan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Synthesis of five different Sudan-β-d-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri-O-acetyl-1-trichloroacetimidoyl-α-d-glucopyran)uronate. After the acid and alcohol groups had been deprotected, the resulting compounds were used for histochemical localization of β-glucuronidase (GUS) activity in transgenic plants (Petunia hybrida, Arabidopsis thaliana, and Nicotiana tabacum) that contained the GUS reporter system. Because the cleavage of the β-glucuronide results in the liberation of an insoluble Sudan dye, Sudan substrates gave no diffusion artifacts as described for the commonly used 5-bromo-4-chloro-3-indolyl-β-d-glucuronide (X-gluc). A comparison of assays with different Sudan glucuronides and X-gluc demonstrated that the SudanIV variant is a valuable glucuronide substrate for the precise histochemical localization of GUS activity in transgenic plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000219
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  • 4
    Electronic Resource
    Electronic Resource
    Both sense and antisense RNAs are targets for the sense transgene-induced posttranscriptional silencing mechanism (2000)
    Springer
    Molecular genetics and genomics 263 (2000), S. 995-1002 
    Details
    ISSN: 1617-4623
    Keywords: Key words Complementary RNA ; Cosuppression ; RNA turnover ; Transgenic plant ; Transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two stable transgenic tobacco lines were obtained as segregants from a primary transformant. Plants homozygous for a T-DNA inverted repeat locus (HOlo1) showed posttranscriptional gene silencing (PTGS) of the neomycin phosphotransferase II (nptII) transgenes, whereas HOlo2 plants, homozygous for a single T-DNA insert, expressed the nptII genes normally. Transient expression of nptII genes newly introduced into leaves of both the HOlo2 and nptII-silenced HOlo1 plants was downregulated only in the silenced background. Different chimeric β-glucuronidase (gus) genes with parts of the nptII transgene inserted in sense or antisense orientation into the 3′-untranslated region, which encoded transcripts that had homology or complementarity to nptII transcripts, showed reduced transient expression specifically in nptII-silenced tissue. Therefore, we conclude that RNAs of both polarities are targets for PTGS-induced RNA degradation, which supports the notion that double-stranded RNA acts as an inducing signal for silencing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008700
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    Paper (German National Licenses)
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