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  • 2000-2004  (6)
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  • 1
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 53 (2004), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A quantitative-competitive PCR (QC-PCR) method with enzyme-linked oligosorbent assay (ELOSA) was developed to monitor Pichia anomala strain K, a biocontrol agent against postharvest diseases on apples. The procedure involved: (i) extraction of strain K DNA; (ii) coamplification of a constant amount of the extracted DNA (containing a strain K DNA marker of 262 base pairs (bp) specifically amplified with SCAR primers K1 and K2) with an internal standard (IS) titration; and (iii) differential hybridization with two specific biotinylated probes either for the target or for the IS sequence on microplate. The IS sequence differed from the target by only a short internal region of 35 bp providing the differential detection between both sequences. Both target and IS sequences proved to be competitive in PCR as well as in sandwich hybridization. Two copies of the target sequence were detected in the strain K genome by means of enzymatic restriction and Southern blot analysis. Varying amounts of strain K cell suspension were quantified in the phosphate buffer used for recovering cells from the apple surface. An accurate estimate of the strain K population was obtained from 103 to 106 yeast cells per apple. The threshold of the method was found to be at 1000 colony-forming units per apple, which was around 100 times more sensitive than the plating method for monitoring strain K.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Applied crystallography online 34 (2001), S. 1-6 
    ISSN: 1600-5767
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: The crystal structure of monoclinic KGd(WO4)2 (KGW) has been refined at room temperature by using single-crystal X-ray diffraction data. The unit-cell parameters are a = 10.652 (4), b = 10.374 (6), c = 7.582 (2) Å, β = 130.80 (2)°, with Z = 4, in space group C2/c. The linear thermal expansion tensor has been determined and the principal axes are [302], [010] and [106]. The principal axis with maximum thermal expansion (\boldalpha'_{33} = 23.44 × 10−6 K−1), {\bf X}'_{3}, was located 12° from the c axis. Undoped crystals of KGW and crystals that were partially doped by Pr, Nd, Ho, Er, Tm and Yb were grown by the top-seeding-solution growth slow-cooling method. The effect of doping on the KGW structure was observed in the cell parameters and in morphological changes. The changes in parameters follow the changes in lanthanide ionic radii. The doped crystals show {021} and {\bar{2}21} faces in addition to the {110}, {\bar{1}11}, {010}, {130} and {310} faces which basically follow the habit of the undoped KGW crystals. The development of the faces is related to the number of the most important periodic bond chains parallel to them.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    Kybernetes 31 (2002), S. 115-123 
    ISSN: 0368-492X
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Computer Science
    Notes: If an autonomous vehicle is working in an image-based system which needs real-time answers and whose response is critical, it will be very important to reduce computation times and, as we know, this could be performed by increasing the system parallelism. Since morphological filtering is the origin of several applications in computer vision, in this paper we are going to describe some new features to implement morphological operations by using digital signal processors. After that, an application to path planning is proposed. The standard shortest path planning problem determines a collision-free path of shortest distance between two distinct locations in an environment scattered with obstacles. Consequently, a path planning algorithm which uses morphological operations and a DSP to process images is then described.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    Kybernetes 33 (2004), S. 133-140 
    ISSN: 0368-492X
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Computer Science
    Notes: In most conventional pattern recognition methods, the first step is extracting features from objects. These features are always expressed in the form of feature vectors. Then, the distribution of feature vectors is estimated for each category. Finally, an unknown input pattern is assigned to the category with the maximum probability. In this work, we present a system that is able to recognize objects according to the likeness of feature vectors. A database, which consists of images that were identified from a vector of related features, is used by the system to discover these objects. If the resulting image features are compared with the ones included in the database, we obtain the object that has the highest similarity with the one we proposed. The probability of success using our feature vector has been very high.
    Type of Medium: Electronic Resource
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