ISSN:
1365-3059
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
An RT–DIAPOPS (reverse transcription–detection of immobilized, amplified product in a one-phase system) assay was developed for the detection of potato virus Y (PVY). The sensitivity of the assay was 0·5 pg when virions were added directly to the RT reaction. The assay was capable of detecting all isolates investigated, representing several taxonomic groups of PVY. The RT–DIAPOPS offers an attractive alternative to RT–polymerase chain reaction (PCR) as it does not depend on gel electrophoresis to detect virus-specific PCR products. Finally, as amplicons are covalently attached to a solid phase, it minimizes the risk of contamination.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1365-3059.2001.00527.x
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